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Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying bioche...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037233/ https://www.ncbi.nlm.nih.gov/pubmed/35480048 http://dx.doi.org/10.3389/fphys.2022.875189 |
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author | Chambrion, Charlotte Depond, Mallorie Angella, Lucia Mouri, Oussama Kendjo, Eric Fricot-Monsinjon, Aurélie Roussel, Camille Biligui, Sylvestre Tantaoui, Ilhame Taieb, Aida Argy, Nicolas Houzé, Sandrine Piarroux, Renaud Siriez, Jean-Yves Jaureguiberry, Stéphane Larréché, Sébastien Théllier, Marc Cenac, Nicolas Buffet, Pierre Ndour, Papa Alioune |
author_facet | Chambrion, Charlotte Depond, Mallorie Angella, Lucia Mouri, Oussama Kendjo, Eric Fricot-Monsinjon, Aurélie Roussel, Camille Biligui, Sylvestre Tantaoui, Ilhame Taieb, Aida Argy, Nicolas Houzé, Sandrine Piarroux, Renaud Siriez, Jean-Yves Jaureguiberry, Stéphane Larréché, Sébastien Théllier, Marc Cenac, Nicolas Buffet, Pierre Ndour, Papa Alioune |
author_sort | Chambrion, Charlotte |
collection | PubMed |
description | In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying biochemical and mechanical alterations during severe malaria treated with artemisinin derivatives, a drug family that induce “pitting” in the spleen. In this study, the hemoglobin concentration dropped by 1.93 G/dl during therapy. During the same period, iRBC accounting for 6.12% of all RBC before therapy (BT) were replaced by pitted-RBC, accounting for 5.33% of RBC after therapy (AT). RBC loss was thus of 15.9%, of which only a minor part was due to the loss of iRBC or pitted-RBC. When comparing RBC BT and AT to normal controls, lipidomics revealed an increase in the cholesterol/phosphatidylethanolamine ratio (0.17 versus 0.24, p < 0.001) and cholesterol/phosphatidylinositol ratio (0.36 versus 0.67, p = 0.001). Using ektacytometry, we observed a reduced deformability of circulating RBC, similar BT and AT, compared to health control donors. The mean Elongation Index at 1.69Pa was 0.24 BT and 0.23 AT vs. 0.28 in controls (p < 0.0001). At 30Pa EI was 0.56 BT and 0.56 AT vs. 0.60 in controls (p < 0.001). The retention rate (rr) of RBC subpopulations in spleen-mimetic microsphere layers was higher for iRBC (rr = 20% p = 0.0033) and pitted-RBC (rr = 19%, p = 0.0031) than for healthy RBC (0.12%). Somewhat surprisingly, the post-treatment anemia in malaria results from the elimination of RBC that were never infected. |
format | Online Article Text |
id | pubmed-9037233 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90372332022-04-26 Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria Chambrion, Charlotte Depond, Mallorie Angella, Lucia Mouri, Oussama Kendjo, Eric Fricot-Monsinjon, Aurélie Roussel, Camille Biligui, Sylvestre Tantaoui, Ilhame Taieb, Aida Argy, Nicolas Houzé, Sandrine Piarroux, Renaud Siriez, Jean-Yves Jaureguiberry, Stéphane Larréché, Sébastien Théllier, Marc Cenac, Nicolas Buffet, Pierre Ndour, Papa Alioune Front Physiol Physiology In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying biochemical and mechanical alterations during severe malaria treated with artemisinin derivatives, a drug family that induce “pitting” in the spleen. In this study, the hemoglobin concentration dropped by 1.93 G/dl during therapy. During the same period, iRBC accounting for 6.12% of all RBC before therapy (BT) were replaced by pitted-RBC, accounting for 5.33% of RBC after therapy (AT). RBC loss was thus of 15.9%, of which only a minor part was due to the loss of iRBC or pitted-RBC. When comparing RBC BT and AT to normal controls, lipidomics revealed an increase in the cholesterol/phosphatidylethanolamine ratio (0.17 versus 0.24, p < 0.001) and cholesterol/phosphatidylinositol ratio (0.36 versus 0.67, p = 0.001). Using ektacytometry, we observed a reduced deformability of circulating RBC, similar BT and AT, compared to health control donors. The mean Elongation Index at 1.69Pa was 0.24 BT and 0.23 AT vs. 0.28 in controls (p < 0.0001). At 30Pa EI was 0.56 BT and 0.56 AT vs. 0.60 in controls (p < 0.001). The retention rate (rr) of RBC subpopulations in spleen-mimetic microsphere layers was higher for iRBC (rr = 20% p = 0.0033) and pitted-RBC (rr = 19%, p = 0.0031) than for healthy RBC (0.12%). Somewhat surprisingly, the post-treatment anemia in malaria results from the elimination of RBC that were never infected. Frontiers Media S.A. 2022-04-05 /pmc/articles/PMC9037233/ /pubmed/35480048 http://dx.doi.org/10.3389/fphys.2022.875189 Text en Copyright © 2022 Chambrion, Depond, Angella, Mouri, Kendjo, Fricot-Monsinjon, Roussel, Biligui, Tantaoui, Taieb, Argy, Houzé, Piarroux, Siriez, Jaureguiberry, Larréché, Théllier, Cenac, Buffet and Ndour. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Chambrion, Charlotte Depond, Mallorie Angella, Lucia Mouri, Oussama Kendjo, Eric Fricot-Monsinjon, Aurélie Roussel, Camille Biligui, Sylvestre Tantaoui, Ilhame Taieb, Aida Argy, Nicolas Houzé, Sandrine Piarroux, Renaud Siriez, Jean-Yves Jaureguiberry, Stéphane Larréché, Sébastien Théllier, Marc Cenac, Nicolas Buffet, Pierre Ndour, Papa Alioune Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria |
title | Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria |
title_full | Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria |
title_fullStr | Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria |
title_full_unstemmed | Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria |
title_short | Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria |
title_sort | altered subpopulations of red blood cells and post-treatment anemia in malaria |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037233/ https://www.ncbi.nlm.nih.gov/pubmed/35480048 http://dx.doi.org/10.3389/fphys.2022.875189 |
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