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Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria

In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying bioche...

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Autores principales: Chambrion, Charlotte, Depond, Mallorie, Angella, Lucia, Mouri, Oussama, Kendjo, Eric, Fricot-Monsinjon, Aurélie, Roussel, Camille, Biligui, Sylvestre, Tantaoui, Ilhame, Taieb, Aida, Argy, Nicolas, Houzé, Sandrine, Piarroux, Renaud, Siriez, Jean-Yves, Jaureguiberry, Stéphane, Larréché, Sébastien, Théllier, Marc, Cenac, Nicolas, Buffet, Pierre, Ndour, Papa Alioune
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037233/
https://www.ncbi.nlm.nih.gov/pubmed/35480048
http://dx.doi.org/10.3389/fphys.2022.875189
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author Chambrion, Charlotte
Depond, Mallorie
Angella, Lucia
Mouri, Oussama
Kendjo, Eric
Fricot-Monsinjon, Aurélie
Roussel, Camille
Biligui, Sylvestre
Tantaoui, Ilhame
Taieb, Aida
Argy, Nicolas
Houzé, Sandrine
Piarroux, Renaud
Siriez, Jean-Yves
Jaureguiberry, Stéphane
Larréché, Sébastien
Théllier, Marc
Cenac, Nicolas
Buffet, Pierre
Ndour, Papa Alioune
author_facet Chambrion, Charlotte
Depond, Mallorie
Angella, Lucia
Mouri, Oussama
Kendjo, Eric
Fricot-Monsinjon, Aurélie
Roussel, Camille
Biligui, Sylvestre
Tantaoui, Ilhame
Taieb, Aida
Argy, Nicolas
Houzé, Sandrine
Piarroux, Renaud
Siriez, Jean-Yves
Jaureguiberry, Stéphane
Larréché, Sébastien
Théllier, Marc
Cenac, Nicolas
Buffet, Pierre
Ndour, Papa Alioune
author_sort Chambrion, Charlotte
collection PubMed
description In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying biochemical and mechanical alterations during severe malaria treated with artemisinin derivatives, a drug family that induce “pitting” in the spleen. In this study, the hemoglobin concentration dropped by 1.93 G/dl during therapy. During the same period, iRBC accounting for 6.12% of all RBC before therapy (BT) were replaced by pitted-RBC, accounting for 5.33% of RBC after therapy (AT). RBC loss was thus of 15.9%, of which only a minor part was due to the loss of iRBC or pitted-RBC. When comparing RBC BT and AT to normal controls, lipidomics revealed an increase in the cholesterol/phosphatidylethanolamine ratio (0.17 versus 0.24, p < 0.001) and cholesterol/phosphatidylinositol ratio (0.36 versus 0.67, p = 0.001). Using ektacytometry, we observed a reduced deformability of circulating RBC, similar BT and AT, compared to health control donors. The mean Elongation Index at 1.69Pa was 0.24 BT and 0.23 AT vs. 0.28 in controls (p < 0.0001). At 30Pa EI was 0.56 BT and 0.56 AT vs. 0.60 in controls (p < 0.001). The retention rate (rr) of RBC subpopulations in spleen-mimetic microsphere layers was higher for iRBC (rr = 20% p = 0.0033) and pitted-RBC (rr = 19%, p = 0.0031) than for healthy RBC (0.12%). Somewhat surprisingly, the post-treatment anemia in malaria results from the elimination of RBC that were never infected.
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spelling pubmed-90372332022-04-26 Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria Chambrion, Charlotte Depond, Mallorie Angella, Lucia Mouri, Oussama Kendjo, Eric Fricot-Monsinjon, Aurélie Roussel, Camille Biligui, Sylvestre Tantaoui, Ilhame Taieb, Aida Argy, Nicolas Houzé, Sandrine Piarroux, Renaud Siriez, Jean-Yves Jaureguiberry, Stéphane Larréché, Sébastien Théllier, Marc Cenac, Nicolas Buffet, Pierre Ndour, Papa Alioune Front Physiol Physiology In acute malaria, the bulk of erythrocyte loss occurs after therapy, with a nadir of hemoglobin generally observed 3–7 days after treatment. The fine mechanisms leading to this early post-treatment anemia are still elusive. We explored pathological changes in RBC subpopulations by quantifying biochemical and mechanical alterations during severe malaria treated with artemisinin derivatives, a drug family that induce “pitting” in the spleen. In this study, the hemoglobin concentration dropped by 1.93 G/dl during therapy. During the same period, iRBC accounting for 6.12% of all RBC before therapy (BT) were replaced by pitted-RBC, accounting for 5.33% of RBC after therapy (AT). RBC loss was thus of 15.9%, of which only a minor part was due to the loss of iRBC or pitted-RBC. When comparing RBC BT and AT to normal controls, lipidomics revealed an increase in the cholesterol/phosphatidylethanolamine ratio (0.17 versus 0.24, p < 0.001) and cholesterol/phosphatidylinositol ratio (0.36 versus 0.67, p = 0.001). Using ektacytometry, we observed a reduced deformability of circulating RBC, similar BT and AT, compared to health control donors. The mean Elongation Index at 1.69Pa was 0.24 BT and 0.23 AT vs. 0.28 in controls (p < 0.0001). At 30Pa EI was 0.56 BT and 0.56 AT vs. 0.60 in controls (p < 0.001). The retention rate (rr) of RBC subpopulations in spleen-mimetic microsphere layers was higher for iRBC (rr = 20% p = 0.0033) and pitted-RBC (rr = 19%, p = 0.0031) than for healthy RBC (0.12%). Somewhat surprisingly, the post-treatment anemia in malaria results from the elimination of RBC that were never infected. Frontiers Media S.A. 2022-04-05 /pmc/articles/PMC9037233/ /pubmed/35480048 http://dx.doi.org/10.3389/fphys.2022.875189 Text en Copyright © 2022 Chambrion, Depond, Angella, Mouri, Kendjo, Fricot-Monsinjon, Roussel, Biligui, Tantaoui, Taieb, Argy, Houzé, Piarroux, Siriez, Jaureguiberry, Larréché, Théllier, Cenac, Buffet and Ndour. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Chambrion, Charlotte
Depond, Mallorie
Angella, Lucia
Mouri, Oussama
Kendjo, Eric
Fricot-Monsinjon, Aurélie
Roussel, Camille
Biligui, Sylvestre
Tantaoui, Ilhame
Taieb, Aida
Argy, Nicolas
Houzé, Sandrine
Piarroux, Renaud
Siriez, Jean-Yves
Jaureguiberry, Stéphane
Larréché, Sébastien
Théllier, Marc
Cenac, Nicolas
Buffet, Pierre
Ndour, Papa Alioune
Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
title Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
title_full Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
title_fullStr Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
title_full_unstemmed Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
title_short Altered Subpopulations of Red Blood Cells and Post-treatment Anemia in Malaria
title_sort altered subpopulations of red blood cells and post-treatment anemia in malaria
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9037233/
https://www.ncbi.nlm.nih.gov/pubmed/35480048
http://dx.doi.org/10.3389/fphys.2022.875189
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