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Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples

Fast and simple detection of C-reactive protein (CRP) is highly significant for the diagnosis and prognosis of inflammatory or infectious diseases. Lateral flow immunoassay has the advantages of rapid detection, simple operation and low cost, but it is usually limited by the quantitative ability and...

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Autores principales: Pang, Renzhu, Zhu, Qunyan, Wei, Jia, Wang, Yaoqi, Xu, Fengqin, Meng, Xianying, Wang, Zhenxin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038069/
https://www.ncbi.nlm.nih.gov/pubmed/35480760
http://dx.doi.org/10.1039/d1ra04404d
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author Pang, Renzhu
Zhu, Qunyan
Wei, Jia
Wang, Yaoqi
Xu, Fengqin
Meng, Xianying
Wang, Zhenxin
author_facet Pang, Renzhu
Zhu, Qunyan
Wei, Jia
Wang, Yaoqi
Xu, Fengqin
Meng, Xianying
Wang, Zhenxin
author_sort Pang, Renzhu
collection PubMed
description Fast and simple detection of C-reactive protein (CRP) is highly significant for the diagnosis and prognosis of inflammatory or infectious diseases. Lateral flow immunoassay has the advantages of rapid detection, simple operation and low cost, but it is usually limited by the quantitative ability and speed of data extraction. Herein, a gold-nanorod-based lateral flow immunoassay was developed to rapidly detect CRP by simultaneously monitoring the colorimetric and temperature signals. In this method, anti-CRP antibody-modified gold nanorods (GNRs) were designed as colorimetric and photothermal conversion probes. A mouse anti-CRP monoclonal antibody and goat anti-mouse IgG were used as test and control lines, respectively. Then, a lateral flow immunochromatographic strip was constructed by a sandwich-type method for detecting CRP by introducing antibody-modified GNRs, and this procedure needed less than 15 min. Finally, the detection signals can be directly observed by eyes and directly read using a thermal imager. The as-synthesized GNR showed high photothermal conversion efficiency (η = 39%) and strong localized surface plasmon resonance (LSPR) absorption. For CRP detection, the proposed immunochromatographic strip exhibited good specificity, high sensitivity, good linearity within the range of 50–10 000 ng mL(−1) and a low limit of detection (LOD, 1.3 ng mL(−1)). This method was successfully applied for CRP detection in clinical plasma samples, and it correlated very well with the diagnostic kit of immunoturbidimetry (r = 0.96). The results indicated that the developed GNR-based immunochromatographic strip has immense potential for use as a rapid and cost-effective in vitro diagnostic kit.
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spelling pubmed-90380692022-04-26 Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples Pang, Renzhu Zhu, Qunyan Wei, Jia Wang, Yaoqi Xu, Fengqin Meng, Xianying Wang, Zhenxin RSC Adv Chemistry Fast and simple detection of C-reactive protein (CRP) is highly significant for the diagnosis and prognosis of inflammatory or infectious diseases. Lateral flow immunoassay has the advantages of rapid detection, simple operation and low cost, but it is usually limited by the quantitative ability and speed of data extraction. Herein, a gold-nanorod-based lateral flow immunoassay was developed to rapidly detect CRP by simultaneously monitoring the colorimetric and temperature signals. In this method, anti-CRP antibody-modified gold nanorods (GNRs) were designed as colorimetric and photothermal conversion probes. A mouse anti-CRP monoclonal antibody and goat anti-mouse IgG were used as test and control lines, respectively. Then, a lateral flow immunochromatographic strip was constructed by a sandwich-type method for detecting CRP by introducing antibody-modified GNRs, and this procedure needed less than 15 min. Finally, the detection signals can be directly observed by eyes and directly read using a thermal imager. The as-synthesized GNR showed high photothermal conversion efficiency (η = 39%) and strong localized surface plasmon resonance (LSPR) absorption. For CRP detection, the proposed immunochromatographic strip exhibited good specificity, high sensitivity, good linearity within the range of 50–10 000 ng mL(−1) and a low limit of detection (LOD, 1.3 ng mL(−1)). This method was successfully applied for CRP detection in clinical plasma samples, and it correlated very well with the diagnostic kit of immunoturbidimetry (r = 0.96). The results indicated that the developed GNR-based immunochromatographic strip has immense potential for use as a rapid and cost-effective in vitro diagnostic kit. The Royal Society of Chemistry 2021-08-23 /pmc/articles/PMC9038069/ /pubmed/35480760 http://dx.doi.org/10.1039/d1ra04404d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Pang, Renzhu
Zhu, Qunyan
Wei, Jia
Wang, Yaoqi
Xu, Fengqin
Meng, Xianying
Wang, Zhenxin
Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples
title Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples
title_full Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples
title_fullStr Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples
title_full_unstemmed Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples
title_short Development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of C-reactive protein in clinical plasma samples
title_sort development of a gold-nanorod-based lateral flow immunoassay for a fast and dual-modal detection of c-reactive protein in clinical plasma samples
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038069/
https://www.ncbi.nlm.nih.gov/pubmed/35480760
http://dx.doi.org/10.1039/d1ra04404d
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