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Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells

Sepsis is caused by bacterial infections or viral infections. Clinically, there exist confirmed or highly suspected infection foci. Mortality caused by septic shock remains in a high rate even though antibiotic treatment works effectively. In this study, we treat THP-1 cells with 1 ug/mL LPS (lipopo...

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Detalles Bibliográficos
Autores principales: Cao, Li, Yang, Kerong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038380/
https://www.ncbi.nlm.nih.gov/pubmed/35480155
http://dx.doi.org/10.1155/2022/7051643
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author Cao, Li
Yang, Kerong
author_facet Cao, Li
Yang, Kerong
author_sort Cao, Li
collection PubMed
description Sepsis is caused by bacterial infections or viral infections. Clinically, there exist confirmed or highly suspected infection foci. Mortality caused by septic shock remains in a high rate even though antibiotic treatment works effectively. In this study, we treat THP-1 cells with 1 ug/mL LPS (lipopolysaccharide) and add paeoniflorin or LR-12 inhibitor. TREM-1 (triggering receptor expressed on myeloid cells-1), IL-6, IL-1β, and TNF-α (tumour necrosis factor alpha (a)-cachectin) were detected by ELISA and qRT-PCR, and western blotting is performed to detect related proteins in the NF-κB signaling pathway. As a result, paeoniflorin can significantly reduce the production of LPS-stimulated TREM-1 as well as inflammatory factors and attenuate the phosphorylation of NF-κB signaling pathway-related factors, such as p65 and IκBα. At the same time, the combined effect of paeoniflorin and LR-12 is more significant. The results of this study solidly prove that paeoniflorin plays a role in inhibiting TREM-1-mediated inflammation and the NF-κB pathway could be a potential mechanism of action.
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spelling pubmed-90383802022-04-26 Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells Cao, Li Yang, Kerong J Healthc Eng Research Article Sepsis is caused by bacterial infections or viral infections. Clinically, there exist confirmed or highly suspected infection foci. Mortality caused by septic shock remains in a high rate even though antibiotic treatment works effectively. In this study, we treat THP-1 cells with 1 ug/mL LPS (lipopolysaccharide) and add paeoniflorin or LR-12 inhibitor. TREM-1 (triggering receptor expressed on myeloid cells-1), IL-6, IL-1β, and TNF-α (tumour necrosis factor alpha (a)-cachectin) were detected by ELISA and qRT-PCR, and western blotting is performed to detect related proteins in the NF-κB signaling pathway. As a result, paeoniflorin can significantly reduce the production of LPS-stimulated TREM-1 as well as inflammatory factors and attenuate the phosphorylation of NF-κB signaling pathway-related factors, such as p65 and IκBα. At the same time, the combined effect of paeoniflorin and LR-12 is more significant. The results of this study solidly prove that paeoniflorin plays a role in inhibiting TREM-1-mediated inflammation and the NF-κB pathway could be a potential mechanism of action. Hindawi 2022-04-18 /pmc/articles/PMC9038380/ /pubmed/35480155 http://dx.doi.org/10.1155/2022/7051643 Text en Copyright © 2022 Li Cao and Kerong Yang. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cao, Li
Yang, Kerong
Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells
title Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells
title_full Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells
title_fullStr Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells
title_full_unstemmed Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells
title_short Paeoniflorin Attenuated TREM-1-Mediated Inflammation in THP-1 Cells
title_sort paeoniflorin attenuated trem-1-mediated inflammation in thp-1 cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038380/
https://www.ncbi.nlm.nih.gov/pubmed/35480155
http://dx.doi.org/10.1155/2022/7051643
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