CRISPRi-mediated transcriptional silencing in iPSCs for the study of human brain development

This protocol describes the design and use of CRISPRi-mediated transcriptional silencing in human iPSCs, for loss-of-function studies in brain development research. The protocol avoids single cell selection, thereby eliminating side effects of clonal expansion and sites of viral integration. We also...

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Detalles Bibliográficos
Autores principales: Johansson, Pia Annette, Adami, Anita, Jakobsson, Johan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038555/
https://www.ncbi.nlm.nih.gov/pubmed/35496780
http://dx.doi.org/10.1016/j.xpro.2022.101285

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