An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines

The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is designed...

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Detalles Bibliográficos
Autores principales: Kar, Bibekananda, Sabharwal, Ankit, Restrepo-Castillo, Santiago, Simone, Brandon W., Clark, Karl J., Ekker, Stephen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038556/
https://www.ncbi.nlm.nih.gov/pubmed/35496789
http://dx.doi.org/10.1016/j.xpro.2022.101288
Descripción
Sumario:The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is designed to be easily modified to work in diverse applications where editing of mitochondrial DNA is desired. For complete details on the use and execution of this protocol, please refer to Sabharwal et al. (2021) and Ma et al. (2016).

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