An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines

The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is designed...

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Detalles Bibliográficos
Autores principales: Kar, Bibekananda, Sabharwal, Ankit, Restrepo-Castillo, Santiago, Simone, Brandon W., Clark, Karl J., Ekker, Stephen C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038556/
https://www.ncbi.nlm.nih.gov/pubmed/35496789
http://dx.doi.org/10.1016/j.xpro.2022.101288

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038556/
https://www.ncbi.nlm.nih.gov/pubmed/35496789
http://dx.doi.org/10.1016/j.xpro.2022.101288

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