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Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology
Moderate leaf rolling helps to form the ideotype of rice. In this study, six independent OsRUS1-GFP overexpression (OsRUS1-OX) transgenic rice lines with rapid and dynamic leaf rolling phenotype in response to sunlight were constructed. However, the mechanism is unknown. Here, RNA-Seq approach was u...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038715/ https://www.ncbi.nlm.nih.gov/pubmed/35468979 http://dx.doi.org/10.1038/s41598-022-10784-x |
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author | Yu, Ning Liang, Yaping Wang, Qingping Peng, Xinxiang He, Zhenghui Hou, Xuewen |
author_facet | Yu, Ning Liang, Yaping Wang, Qingping Peng, Xinxiang He, Zhenghui Hou, Xuewen |
author_sort | Yu, Ning |
collection | PubMed |
description | Moderate leaf rolling helps to form the ideotype of rice. In this study, six independent OsRUS1-GFP overexpression (OsRUS1-OX) transgenic rice lines with rapid and dynamic leaf rolling phenotype in response to sunlight were constructed. However, the mechanism is unknown. Here, RNA-Seq approach was utilized to identify differentially expressed genes between flag leaves of OsRUS1-OX and wildtype under sunlight. 2920 genes were differentially expressed between OsRUS1-OX and WT, of which 1660 upregulated and 1260 downregulated. Six of the 16 genes in GO: 0009415 (response to water stimulus) were significantly upregulated in OsRUS1-OX. The differentially expressed genes between WT and OsRUS1-OX were assigned to 110 KEGG pathways. 42 of the 222 genes in KEGG pathway dosa04075 (Plant hormone signal transduction) were differentially expressed between WT and OsRUS1-OX. The identified genes in GO:0009415 and KEGG pathway dosa04075 were good candidates to explain the leaf rolling phenotype of OsRUS1-OX. The expression patterns of the 15 genes identified by RNA-Seq were verified by qRT-PCR. Based on transcriptomic and qRT-PCR analysis, a mechanism for the leaf rolling phenotype of OsRUS1-OX was proposed. The differential expression profiles between WT and OsRUS1-OX established by this study provide important insights into the molecular mechanism behind the leaf rolling phenotype of OsRUS1-OX. |
format | Online Article Text |
id | pubmed-9038715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90387152022-04-27 Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology Yu, Ning Liang, Yaping Wang, Qingping Peng, Xinxiang He, Zhenghui Hou, Xuewen Sci Rep Article Moderate leaf rolling helps to form the ideotype of rice. In this study, six independent OsRUS1-GFP overexpression (OsRUS1-OX) transgenic rice lines with rapid and dynamic leaf rolling phenotype in response to sunlight were constructed. However, the mechanism is unknown. Here, RNA-Seq approach was utilized to identify differentially expressed genes between flag leaves of OsRUS1-OX and wildtype under sunlight. 2920 genes were differentially expressed between OsRUS1-OX and WT, of which 1660 upregulated and 1260 downregulated. Six of the 16 genes in GO: 0009415 (response to water stimulus) were significantly upregulated in OsRUS1-OX. The differentially expressed genes between WT and OsRUS1-OX were assigned to 110 KEGG pathways. 42 of the 222 genes in KEGG pathway dosa04075 (Plant hormone signal transduction) were differentially expressed between WT and OsRUS1-OX. The identified genes in GO:0009415 and KEGG pathway dosa04075 were good candidates to explain the leaf rolling phenotype of OsRUS1-OX. The expression patterns of the 15 genes identified by RNA-Seq were verified by qRT-PCR. Based on transcriptomic and qRT-PCR analysis, a mechanism for the leaf rolling phenotype of OsRUS1-OX was proposed. The differential expression profiles between WT and OsRUS1-OX established by this study provide important insights into the molecular mechanism behind the leaf rolling phenotype of OsRUS1-OX. Nature Publishing Group UK 2022-04-25 /pmc/articles/PMC9038715/ /pubmed/35468979 http://dx.doi.org/10.1038/s41598-022-10784-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Yu, Ning Liang, Yaping Wang, Qingping Peng, Xinxiang He, Zhenghui Hou, Xuewen Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology |
title | Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology |
title_full | Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology |
title_fullStr | Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology |
title_full_unstemmed | Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology |
title_short | Transcriptomic analysis of OsRUS1 overexpression rice lines with rapid and dynamic leaf rolling morphology |
title_sort | transcriptomic analysis of osrus1 overexpression rice lines with rapid and dynamic leaf rolling morphology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038715/ https://www.ncbi.nlm.nih.gov/pubmed/35468979 http://dx.doi.org/10.1038/s41598-022-10784-x |
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