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Assessing water permeability of aquaporins in a proteoliposome-based stopped-flow setup

Aquaporins (AQPs) are water channels embedded in the cell membrane that are critical in maintaining water homeostasis. We describe a protocol for determining the water permeation capacity of AQPs reconstituted into proteoliposomes. Using a stopped-flow setup, AQP embedded in proteoliposomes are expo...

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Detalles Bibliográficos
Autores principales:	Steffen, Jonas Hyld, Missel, Julie Winkel, Al-Jubair, Tamim, Kitchen, Philip, Salman, Mootaz M., Bill, Roslyn M., Törnroth-Horsefield, Susanna, Gourdon, Pontus
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038760/ https://www.ncbi.nlm.nih.gov/pubmed/35496800 http://dx.doi.org/10.1016/j.xpro.2022.101312

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