Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells

In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1...

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Detalles Bibliográficos
Autores principales: Yang, Jiwen, Hu, Minjie, Wang, Yongyu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038768/
https://www.ncbi.nlm.nih.gov/pubmed/35496784
http://dx.doi.org/10.1016/j.xpro.2022.101296
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author Yang, Jiwen
Hu, Minjie
Wang, Yongyu
author_facet Yang, Jiwen
Hu, Minjie
Wang, Yongyu
author_sort Yang, Jiwen
collection PubMed
description In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs. For complete details on the use and execution of this protocol, please refer to Quan et al. (2021).
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spelling pubmed-90387682022-04-27 Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells Yang, Jiwen Hu, Minjie Wang, Yongyu STAR Protoc Protocol In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1 and/or YAP in H1 embryonic stem cells (H1 ESCs) as an example. Our protocol can be applied to any gene of interest in a variety of hPSCs. For complete details on the use and execution of this protocol, please refer to Quan et al. (2021). Elsevier 2022-04-13 /pmc/articles/PMC9038768/ /pubmed/35496784 http://dx.doi.org/10.1016/j.xpro.2022.101296 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yang, Jiwen
Hu, Minjie
Wang, Yongyu
Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
title Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
title_full Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
title_fullStr Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
title_full_unstemmed Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
title_short Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
title_sort protocol for inducible piggybac transposon system for efficient gene overexpression in human pluripotent stem cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038768/
https://www.ncbi.nlm.nih.gov/pubmed/35496784
http://dx.doi.org/10.1016/j.xpro.2022.101296
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AT huminjie protocolforinduciblepiggybactransposonsystemforefficientgeneoverexpressioninhumanpluripotentstemcells
AT wangyongyu protocolforinduciblepiggybactransposonsystemforefficientgeneoverexpressioninhumanpluripotentstemcells

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