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Protocol for inducible piggyBac transposon system for efficient gene overexpression in human pluripotent stem cells
In human pluripotent stem cells (hPSCs), traditional approaches for gene overexpression have low efficiency and are often laborious. Here, we provide a relatively simple protocol for gene overexpression with the Dox-inducible PiggyBac transposon system. We detail the steps for overexpression of FLI1...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038768/ https://www.ncbi.nlm.nih.gov/pubmed/35496784 http://dx.doi.org/10.1016/j.xpro.2022.101296 |