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Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease
Activation of innate immune sensors by endogenous DNA and RNA can lead to autoimmune and autoinflammatory diseases. Quantification of the unperturbed phosphoprotein content in immune cells provides insight into the spontaneous activity of immune signaling pathways triggered by nucleic acid recogniti...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038771/ https://www.ncbi.nlm.nih.gov/pubmed/35496781 http://dx.doi.org/10.1016/j.xpro.2022.101309 |
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author | Krollmann, Calvin Cieslak, Kevin Koerber, Ruth-Miriam Luksch, Hella Rösen-Wolff, Angela Brossart, Peter Teichmann, Lino L. |
author_facet | Krollmann, Calvin Cieslak, Kevin Koerber, Ruth-Miriam Luksch, Hella Rösen-Wolff, Angela Brossart, Peter Teichmann, Lino L. |
author_sort | Krollmann, Calvin |
collection | PubMed |
description | Activation of innate immune sensors by endogenous DNA and RNA can lead to autoimmune and autoinflammatory diseases. Quantification of the unperturbed phosphoprotein content in immune cells provides insight into the spontaneous activity of immune signaling pathways triggered by nucleic acid recognition. Here, we present a phosphoflow protocol for measuring phosphoproteins in mouse models of autoimmunity that incorporates strategies to preserve native phosphoprotein levels during sample collection and to reliably detect low signaling activity common in chronic disease states. For complete details on the use and execution of this protocol, please refer to Jütte et al. (2021). |
format | Online Article Text |
id | pubmed-9038771 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90387712022-04-27 Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease Krollmann, Calvin Cieslak, Kevin Koerber, Ruth-Miriam Luksch, Hella Rösen-Wolff, Angela Brossart, Peter Teichmann, Lino L. STAR Protoc Protocol Activation of innate immune sensors by endogenous DNA and RNA can lead to autoimmune and autoinflammatory diseases. Quantification of the unperturbed phosphoprotein content in immune cells provides insight into the spontaneous activity of immune signaling pathways triggered by nucleic acid recognition. Here, we present a phosphoflow protocol for measuring phosphoproteins in mouse models of autoimmunity that incorporates strategies to preserve native phosphoprotein levels during sample collection and to reliably detect low signaling activity common in chronic disease states. For complete details on the use and execution of this protocol, please refer to Jütte et al. (2021). Elsevier 2022-04-13 /pmc/articles/PMC9038771/ /pubmed/35496781 http://dx.doi.org/10.1016/j.xpro.2022.101309 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Krollmann, Calvin Cieslak, Kevin Koerber, Ruth-Miriam Luksch, Hella Rösen-Wolff, Angela Brossart, Peter Teichmann, Lino L. Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease |
title | Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease |
title_full | Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease |
title_fullStr | Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease |
title_full_unstemmed | Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease |
title_short | Quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease |
title_sort | quantification of unperturbed phosphoprotein levels in immune cell subsets with phosphoflow to assess immune signaling in autoimmune disease |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038771/ https://www.ncbi.nlm.nih.gov/pubmed/35496781 http://dx.doi.org/10.1016/j.xpro.2022.101309 |
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