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Effects of Anesthesia on Ozone-Induced Lung and Systemic Inflammation

PURPOSE: Anesthetics are required for procedures that deliver drugs/biologics, infectious/inflammatory agents, and toxicants directly to the lungs. However, the possible confounding effects of anesthesia on lung inflammation and injury are underreported. Here, we evaluated the effects of two commonl...

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Autores principales: Wilson, Miranda L., Thysell, Jarl A., Baumann, Kristen K., Quaranta, Danny V., Liang, W. Sandy, Erickson, Michelle A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038869/
https://www.ncbi.nlm.nih.gov/pubmed/35199228
http://dx.doi.org/10.1007/s00408-022-00514-5
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author Wilson, Miranda L.
Thysell, Jarl A.
Baumann, Kristen K.
Quaranta, Danny V.
Liang, W. Sandy
Erickson, Michelle A.
author_facet Wilson, Miranda L.
Thysell, Jarl A.
Baumann, Kristen K.
Quaranta, Danny V.
Liang, W. Sandy
Erickson, Michelle A.
author_sort Wilson, Miranda L.
collection PubMed
description PURPOSE: Anesthetics are required for procedures that deliver drugs/biologics, infectious/inflammatory agents, and toxicants directly to the lungs. However, the possible confounding effects of anesthesia on lung inflammation and injury are underreported. Here, we evaluated the effects of two commonly used anesthetic regimens on lung inflammatory responses to ozone in mice. METHODS: We tested the effects of brief isoflurane (Iso) or ketamine/xylazine/atipamezole (K/X/A) anesthesia prior to ozone exposure (4 h, 3 ppm) on lung inflammatory responses in mice. Anesthesia regimens modeled those used for non-surgical intratracheal instillations and were administered 1–2 h or 24 h prior to initiating ozone exposure. RESULTS: We found that Iso given 1–2 h prior to ozone inhibited inflammatory responses in the lung, and this effect was absent when Iso was given 23–24 h prior to ozone. In contrast, K/X/A given 1–2 h prior to ozone increased lung and systemic inflammation. CONCLUSION: Our results highlight the need to comprehensively evaluate anesthesia as an experimental variable in the assessment of lung inflammation in response to ozone and other inflammatory stimuli.
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spelling pubmed-90388692022-05-07 Effects of Anesthesia on Ozone-Induced Lung and Systemic Inflammation Wilson, Miranda L. Thysell, Jarl A. Baumann, Kristen K. Quaranta, Danny V. Liang, W. Sandy Erickson, Michelle A. Lung Ozone-Induced Lung Injury PURPOSE: Anesthetics are required for procedures that deliver drugs/biologics, infectious/inflammatory agents, and toxicants directly to the lungs. However, the possible confounding effects of anesthesia on lung inflammation and injury are underreported. Here, we evaluated the effects of two commonly used anesthetic regimens on lung inflammatory responses to ozone in mice. METHODS: We tested the effects of brief isoflurane (Iso) or ketamine/xylazine/atipamezole (K/X/A) anesthesia prior to ozone exposure (4 h, 3 ppm) on lung inflammatory responses in mice. Anesthesia regimens modeled those used for non-surgical intratracheal instillations and were administered 1–2 h or 24 h prior to initiating ozone exposure. RESULTS: We found that Iso given 1–2 h prior to ozone inhibited inflammatory responses in the lung, and this effect was absent when Iso was given 23–24 h prior to ozone. In contrast, K/X/A given 1–2 h prior to ozone increased lung and systemic inflammation. CONCLUSION: Our results highlight the need to comprehensively evaluate anesthesia as an experimental variable in the assessment of lung inflammation in response to ozone and other inflammatory stimuli. Springer US 2022-02-23 2022 /pmc/articles/PMC9038869/ /pubmed/35199228 http://dx.doi.org/10.1007/s00408-022-00514-5 Text en © This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Ozone-Induced Lung Injury
Wilson, Miranda L.
Thysell, Jarl A.
Baumann, Kristen K.
Quaranta, Danny V.
Liang, W. Sandy
Erickson, Michelle A.
Effects of Anesthesia on Ozone-Induced Lung and Systemic Inflammation
title Effects of Anesthesia on Ozone-Induced Lung and Systemic Inflammation
title_full Effects of Anesthesia on Ozone-Induced Lung and Systemic Inflammation
title_fullStr Effects of Anesthesia on Ozone-Induced Lung and Systemic Inflammation
title_full_unstemmed Effects of Anesthesia on Ozone-Induced Lung and Systemic Inflammation
title_short Effects of Anesthesia on Ozone-Induced Lung and Systemic Inflammation
title_sort effects of anesthesia on ozone-induced lung and systemic inflammation
topic Ozone-Induced Lung Injury
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038869/
https://www.ncbi.nlm.nih.gov/pubmed/35199228
http://dx.doi.org/10.1007/s00408-022-00514-5
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