A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein

Plant pathogens degrade cell wall through secreted polygalacturonases (PGs) during infection. Plants counteract the PGs by producing PG-inhibiting proteins (PGIPs) for protection, reversibly binding fungal PGs, and mitigating their hydrolytic activities. To date, how fungal pathogens specifically ov...

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Autores principales: Wei, Wei, Xu, Liangsheng, Peng, Hao, Zhu, Wenjun, Tanaka, Kiwamu, Cheng, Jiasen, Sanguinet, Karen A., Vandemark, George, Chen, Weidong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038911/
https://www.ncbi.nlm.nih.gov/pubmed/35468894
http://dx.doi.org/10.1038/s41467-022-29788-2
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author Wei, Wei
Xu, Liangsheng
Peng, Hao
Zhu, Wenjun
Tanaka, Kiwamu
Cheng, Jiasen
Sanguinet, Karen A.
Vandemark, George
Chen, Weidong
author_facet Wei, Wei
Xu, Liangsheng
Peng, Hao
Zhu, Wenjun
Tanaka, Kiwamu
Cheng, Jiasen
Sanguinet, Karen A.
Vandemark, George
Chen, Weidong
author_sort Wei, Wei
collection PubMed
description Plant pathogens degrade cell wall through secreted polygalacturonases (PGs) during infection. Plants counteract the PGs by producing PG-inhibiting proteins (PGIPs) for protection, reversibly binding fungal PGs, and mitigating their hydrolytic activities. To date, how fungal pathogens specifically overcome PGIP inhibition is unknown. Here, we report an effector, Sclerotinia sclerotiorum PGIP-INactivating Effector 1 (SsPINE1), which directly interacts with and functionally inactivates PGIP. S. sclerotiorum is a necrotrophic fungus that causes stem rot diseases on more than 600 plant species with tissue maceration being the most prominent symptom. SsPINE1 enhances S. sclerotiorum necrotrophic virulence by specifically interacting with host PGIPs to negate their polygalacturonase-inhibiting function via enhanced dissociation of PGIPs from PGs. Targeted deletion of SsPINE1 reduces the fungal virulence. Ectopic expression of SsPINE1 in plant reduces its resistance against S. sclerotiorum. Functional and genomic analyses reveal a conserved virulence mechanism of cognate PINE1 proteins in broad host range necrotrophic fungal pathogens.
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spelling pubmed-90389112022-04-28 A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein Wei, Wei Xu, Liangsheng Peng, Hao Zhu, Wenjun Tanaka, Kiwamu Cheng, Jiasen Sanguinet, Karen A. Vandemark, George Chen, Weidong Nat Commun Article Plant pathogens degrade cell wall through secreted polygalacturonases (PGs) during infection. Plants counteract the PGs by producing PG-inhibiting proteins (PGIPs) for protection, reversibly binding fungal PGs, and mitigating their hydrolytic activities. To date, how fungal pathogens specifically overcome PGIP inhibition is unknown. Here, we report an effector, Sclerotinia sclerotiorum PGIP-INactivating Effector 1 (SsPINE1), which directly interacts with and functionally inactivates PGIP. S. sclerotiorum is a necrotrophic fungus that causes stem rot diseases on more than 600 plant species with tissue maceration being the most prominent symptom. SsPINE1 enhances S. sclerotiorum necrotrophic virulence by specifically interacting with host PGIPs to negate their polygalacturonase-inhibiting function via enhanced dissociation of PGIPs from PGs. Targeted deletion of SsPINE1 reduces the fungal virulence. Ectopic expression of SsPINE1 in plant reduces its resistance against S. sclerotiorum. Functional and genomic analyses reveal a conserved virulence mechanism of cognate PINE1 proteins in broad host range necrotrophic fungal pathogens. Nature Publishing Group UK 2022-04-25 /pmc/articles/PMC9038911/ /pubmed/35468894 http://dx.doi.org/10.1038/s41467-022-29788-2 Text en © This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wei, Wei
Xu, Liangsheng
Peng, Hao
Zhu, Wenjun
Tanaka, Kiwamu
Cheng, Jiasen
Sanguinet, Karen A.
Vandemark, George
Chen, Weidong
A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein
title A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein
title_full A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein
title_fullStr A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein
title_full_unstemmed A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein
title_short A fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein
title_sort fungal extracellular effector inactivates plant polygalacturonase-inhibiting protein
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038911/
https://www.ncbi.nlm.nih.gov/pubmed/35468894
http://dx.doi.org/10.1038/s41467-022-29788-2
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