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Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival

There is ample support for developmental regulation of glioblastoma stem cells. To examine how cell lineage controls glioblastoma stem cell function, we present a cross-species epigenome analysis of mouse and human glioblastoma stem cells. We analyze and compare the chromatin-accessibility landscape...

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Autores principales: Lu, Xi, Maturi, Naga Prathyusha, Jarvius, Malin, Yildirim, Irem, Dang, Yonglong, Zhao, Linxuan, Xie, Yuan, Tan, E-Jean, Xing, Pengwei, Larsson, Rolf, Fryknäs, Mårten, Uhrbom, Lene, Chen, Xingqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038925/
https://www.ncbi.nlm.nih.gov/pubmed/35469026
http://dx.doi.org/10.1038/s41467-022-29912-2
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author Lu, Xi
Maturi, Naga Prathyusha
Jarvius, Malin
Yildirim, Irem
Dang, Yonglong
Zhao, Linxuan
Xie, Yuan
Tan, E-Jean
Xing, Pengwei
Larsson, Rolf
Fryknäs, Mårten
Uhrbom, Lene
Chen, Xingqi
author_facet Lu, Xi
Maturi, Naga Prathyusha
Jarvius, Malin
Yildirim, Irem
Dang, Yonglong
Zhao, Linxuan
Xie, Yuan
Tan, E-Jean
Xing, Pengwei
Larsson, Rolf
Fryknäs, Mårten
Uhrbom, Lene
Chen, Xingqi
author_sort Lu, Xi
collection PubMed
description There is ample support for developmental regulation of glioblastoma stem cells. To examine how cell lineage controls glioblastoma stem cell function, we present a cross-species epigenome analysis of mouse and human glioblastoma stem cells. We analyze and compare the chromatin-accessibility landscape of nine mouse glioblastoma stem cell cultures of three defined origins and 60 patient-derived glioblastoma stem cell cultures by assay for transposase-accessible chromatin using sequencing. This separates the mouse cultures according to cell of origin and identifies three human glioblastoma stem cell clusters that show overlapping characteristics with each of the mouse groups, and a distribution along an axis of proneural to mesenchymal phenotypes. The epigenetic-based human glioblastoma stem cell clusters display distinct functional properties and can separate patient survival. Cross-species analyses reveals conserved epigenetic regulation of mouse and human glioblastoma stem cells. We conclude that epigenetic control of glioblastoma stem cells primarily is dictated by developmental origin which impacts clinically relevant glioblastoma stem cell properties and patient survival.
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spelling pubmed-90389252022-04-28 Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival Lu, Xi Maturi, Naga Prathyusha Jarvius, Malin Yildirim, Irem Dang, Yonglong Zhao, Linxuan Xie, Yuan Tan, E-Jean Xing, Pengwei Larsson, Rolf Fryknäs, Mårten Uhrbom, Lene Chen, Xingqi Nat Commun Article There is ample support for developmental regulation of glioblastoma stem cells. To examine how cell lineage controls glioblastoma stem cell function, we present a cross-species epigenome analysis of mouse and human glioblastoma stem cells. We analyze and compare the chromatin-accessibility landscape of nine mouse glioblastoma stem cell cultures of three defined origins and 60 patient-derived glioblastoma stem cell cultures by assay for transposase-accessible chromatin using sequencing. This separates the mouse cultures according to cell of origin and identifies three human glioblastoma stem cell clusters that show overlapping characteristics with each of the mouse groups, and a distribution along an axis of proneural to mesenchymal phenotypes. The epigenetic-based human glioblastoma stem cell clusters display distinct functional properties and can separate patient survival. Cross-species analyses reveals conserved epigenetic regulation of mouse and human glioblastoma stem cells. We conclude that epigenetic control of glioblastoma stem cells primarily is dictated by developmental origin which impacts clinically relevant glioblastoma stem cell properties and patient survival. Nature Publishing Group UK 2022-04-25 /pmc/articles/PMC9038925/ /pubmed/35469026 http://dx.doi.org/10.1038/s41467-022-29912-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Lu, Xi
Maturi, Naga Prathyusha
Jarvius, Malin
Yildirim, Irem
Dang, Yonglong
Zhao, Linxuan
Xie, Yuan
Tan, E-Jean
Xing, Pengwei
Larsson, Rolf
Fryknäs, Mårten
Uhrbom, Lene
Chen, Xingqi
Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival
title Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival
title_full Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival
title_fullStr Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival
title_full_unstemmed Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival
title_short Cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival
title_sort cell-lineage controlled epigenetic regulation in glioblastoma stem cells determines functionally distinct subgroups and predicts patient survival
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9038925/
https://www.ncbi.nlm.nih.gov/pubmed/35469026
http://dx.doi.org/10.1038/s41467-022-29912-2
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