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Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging

Afterglow luminescence is an internal luminescence pathway that occurs after photo-excitation, holds great promise for non-background molecular imaging in vivo, but suffer from poor quantitative ability owing to luminescent attenuation over time. Moreover, the inert structure and insufficient reacti...

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Autores principales: Liu, Yongchao, Teng, Lili, Lyu, Yifan, Song, Guosheng, Zhang, Xiao-Bing, Tan, Weihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039063/
https://www.ncbi.nlm.nih.gov/pubmed/35468901
http://dx.doi.org/10.1038/s41467-022-29894-1
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author Liu, Yongchao
Teng, Lili
Lyu, Yifan
Song, Guosheng
Zhang, Xiao-Bing
Tan, Weihong
author_facet Liu, Yongchao
Teng, Lili
Lyu, Yifan
Song, Guosheng
Zhang, Xiao-Bing
Tan, Weihong
author_sort Liu, Yongchao
collection PubMed
description Afterglow luminescence is an internal luminescence pathway that occurs after photo-excitation, holds great promise for non-background molecular imaging in vivo, but suffer from poor quantitative ability owing to luminescent attenuation over time. Moreover, the inert structure and insufficient reactive sites of current afterglow materials make it hard to design activatable afterglow probes for specific detection. Here, we report a ratiometric afterglow luminescent nanoplatform to customize various activatable afterglow probes for reliable quantification and molecular imaging of specific analytes, such as NO, ONOO(−) or pH. Notably, these afterglow probes can not only address the attenuation of afterglow intensity and eliminate the interference of factors (e.g., laser power, irradiation time, and exposure time), but also significantly improve the imaging reliability in vivo and signal-to-background ratios (~1200-fold), both of which enable more reliable quantitative analysis in biological systems. Moreover, as a proof-of-concept, we successfully design an NO-responsive ratiometric afterglow nanoprobe, RAN1. This nanoprobe can monitor the fluctuations of intratumoral NO, as a biomarker of macrophage polarization, making it possible to real-time dynamically evaluate the degree cancer immunotherapy, which provides a reliable parameter to predict the immunotherapeutic effect.
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spelling pubmed-90390632022-04-28 Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging Liu, Yongchao Teng, Lili Lyu, Yifan Song, Guosheng Zhang, Xiao-Bing Tan, Weihong Nat Commun Article Afterglow luminescence is an internal luminescence pathway that occurs after photo-excitation, holds great promise for non-background molecular imaging in vivo, but suffer from poor quantitative ability owing to luminescent attenuation over time. Moreover, the inert structure and insufficient reactive sites of current afterglow materials make it hard to design activatable afterglow probes for specific detection. Here, we report a ratiometric afterglow luminescent nanoplatform to customize various activatable afterglow probes for reliable quantification and molecular imaging of specific analytes, such as NO, ONOO(−) or pH. Notably, these afterglow probes can not only address the attenuation of afterglow intensity and eliminate the interference of factors (e.g., laser power, irradiation time, and exposure time), but also significantly improve the imaging reliability in vivo and signal-to-background ratios (~1200-fold), both of which enable more reliable quantitative analysis in biological systems. Moreover, as a proof-of-concept, we successfully design an NO-responsive ratiometric afterglow nanoprobe, RAN1. This nanoprobe can monitor the fluctuations of intratumoral NO, as a biomarker of macrophage polarization, making it possible to real-time dynamically evaluate the degree cancer immunotherapy, which provides a reliable parameter to predict the immunotherapeutic effect. Nature Publishing Group UK 2022-04-25 /pmc/articles/PMC9039063/ /pubmed/35468901 http://dx.doi.org/10.1038/s41467-022-29894-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Liu, Yongchao
Teng, Lili
Lyu, Yifan
Song, Guosheng
Zhang, Xiao-Bing
Tan, Weihong
Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging
title Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging
title_full Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging
title_fullStr Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging
title_full_unstemmed Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging
title_short Ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging
title_sort ratiometric afterglow luminescent nanoplatform enables reliable quantification and molecular imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039063/
https://www.ncbi.nlm.nih.gov/pubmed/35468901
http://dx.doi.org/10.1038/s41467-022-29894-1
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