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TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line
Human Papillomavirus is responsible for a wide range of mucosal lesions and tumors. The immune system participate in tumorigenesis in different ways. For example, signaling pathways triggered by Toll-like receptors (TLR) play a role in chemotherapy resistance in several tumor types and are candidate...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039070/ https://www.ncbi.nlm.nih.gov/pubmed/35468924 http://dx.doi.org/10.1038/s41598-022-09980-6 |
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author | Morale, Mirian Galliote Tamura, Rodrigo Esaki Cintra, Ricardo Araújo, Natália Meneses Villa, Luisa Lina |
author_facet | Morale, Mirian Galliote Tamura, Rodrigo Esaki Cintra, Ricardo Araújo, Natália Meneses Villa, Luisa Lina |
author_sort | Morale, Mirian Galliote |
collection | PubMed |
description | Human Papillomavirus is responsible for a wide range of mucosal lesions and tumors. The immune system participate in tumorigenesis in different ways. For example, signaling pathways triggered by Toll-like receptors (TLR) play a role in chemotherapy resistance in several tumor types and are candidates for contributing to the development of HPV-induced tumors. Here, we studied the receptor TLR4 and the adaptor molecule SARM1 in HeLa cells, an HPV-positive cervical cancer cell line. Knocking out of these genes individually proved to be important for maintaining cell viability and proliferation. TLR4 knock out cells were more sensitive to cisplatin treatment, which was illustrated by an increased frequency of apoptotic cells. Furthermore, TLR4 and SARM1 modulated ROS production, which was induced by cell death in response to cisplatin. In conclusion, TLR4 and SARM1 are important for therapy resistance and cervical cancer cell viability and may be relevant clinical targets. |
format | Online Article Text |
id | pubmed-9039070 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90390702022-04-27 TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line Morale, Mirian Galliote Tamura, Rodrigo Esaki Cintra, Ricardo Araújo, Natália Meneses Villa, Luisa Lina Sci Rep Article Human Papillomavirus is responsible for a wide range of mucosal lesions and tumors. The immune system participate in tumorigenesis in different ways. For example, signaling pathways triggered by Toll-like receptors (TLR) play a role in chemotherapy resistance in several tumor types and are candidates for contributing to the development of HPV-induced tumors. Here, we studied the receptor TLR4 and the adaptor molecule SARM1 in HeLa cells, an HPV-positive cervical cancer cell line. Knocking out of these genes individually proved to be important for maintaining cell viability and proliferation. TLR4 knock out cells were more sensitive to cisplatin treatment, which was illustrated by an increased frequency of apoptotic cells. Furthermore, TLR4 and SARM1 modulated ROS production, which was induced by cell death in response to cisplatin. In conclusion, TLR4 and SARM1 are important for therapy resistance and cervical cancer cell viability and may be relevant clinical targets. Nature Publishing Group UK 2022-04-25 /pmc/articles/PMC9039070/ /pubmed/35468924 http://dx.doi.org/10.1038/s41598-022-09980-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Morale, Mirian Galliote Tamura, Rodrigo Esaki Cintra, Ricardo Araújo, Natália Meneses Villa, Luisa Lina TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line |
title | TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line |
title_full | TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line |
title_fullStr | TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line |
title_full_unstemmed | TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line |
title_short | TLR4 and SARM1 modulate survival and chemoresistance in an HPV-positive cervical cancer cell line |
title_sort | tlr4 and sarm1 modulate survival and chemoresistance in an hpv-positive cervical cancer cell line |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039070/ https://www.ncbi.nlm.nih.gov/pubmed/35468924 http://dx.doi.org/10.1038/s41598-022-09980-6 |
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