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Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17

It has long been recognized that enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for vomiting and diarrhea. E. coli F17, a main subtype of ETEC, is characterized by high morbidity and mortality in young livestock. However, the transcriptomic basis underlying E. coli F17 inf...

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Autores principales: Chen, Weihao, Lv, Xiaoyang, Zhang, Weibo, Hu, Tingyan, Cao, Xiukai, Ren, Ziming, Getachew, Tesfaye, Mwacharo, Joram M., Haile, Aynalem, Sun, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039264/
https://www.ncbi.nlm.nih.gov/pubmed/35498757
http://dx.doi.org/10.3389/fvets.2022.819917
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author Chen, Weihao
Lv, Xiaoyang
Zhang, Weibo
Hu, Tingyan
Cao, Xiukai
Ren, Ziming
Getachew, Tesfaye
Mwacharo, Joram M.
Haile, Aynalem
Sun, Wei
author_facet Chen, Weihao
Lv, Xiaoyang
Zhang, Weibo
Hu, Tingyan
Cao, Xiukai
Ren, Ziming
Getachew, Tesfaye
Mwacharo, Joram M.
Haile, Aynalem
Sun, Wei
author_sort Chen, Weihao
collection PubMed
description It has long been recognized that enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for vomiting and diarrhea. E. coli F17, a main subtype of ETEC, is characterized by high morbidity and mortality in young livestock. However, the transcriptomic basis underlying E. coli F17 infection has not been fully understood. In the present study, RNA sequencing was conducted to explore the expression profiles of mRNAs and long non-coding RNAs (lncRNAs) in the jejunum of lambs who were identified as resistant or sensitive to E. coli F17 that was obtained in a challenge experiment. A total of 772 differentially expressed (DE) mRNAs and 190 DE lncRNAs were detected between the E. coli F17—resistance and E. coli F17-sensitive lambs (i.e., TFF2, LOC105606142, OLFM4, LYPD8, REG4, APOA4, TCONS_00223467, and TCONS_00241897). Then, a two-step machine learning approach (RX) combination Random Forest and Extreme Gradient Boosting were performed, which identified 16 mRNAs and 17 lncRNAs as potential biomarkers, within which PPP2R3A and TCONS_00182693 were prioritized as key biomarkers involved in E. coli F17 infection. Furthermore, functional enrichment analysis showed that peroxisome proliferator-activated receptor (PPAR) pathway was significantly enriched in response to E. coli F17 infection. Our finding will help to improve the knowledge of the mechanisms underlying E. coli F17 infection and may provide novel targets for future treatment of E. coli F17 infection.
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spelling pubmed-90392642022-04-27 Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17 Chen, Weihao Lv, Xiaoyang Zhang, Weibo Hu, Tingyan Cao, Xiukai Ren, Ziming Getachew, Tesfaye Mwacharo, Joram M. Haile, Aynalem Sun, Wei Front Vet Sci Veterinary Science It has long been recognized that enterotoxigenic Escherichia coli (ETEC) is the major pathogen responsible for vomiting and diarrhea. E. coli F17, a main subtype of ETEC, is characterized by high morbidity and mortality in young livestock. However, the transcriptomic basis underlying E. coli F17 infection has not been fully understood. In the present study, RNA sequencing was conducted to explore the expression profiles of mRNAs and long non-coding RNAs (lncRNAs) in the jejunum of lambs who were identified as resistant or sensitive to E. coli F17 that was obtained in a challenge experiment. A total of 772 differentially expressed (DE) mRNAs and 190 DE lncRNAs were detected between the E. coli F17—resistance and E. coli F17-sensitive lambs (i.e., TFF2, LOC105606142, OLFM4, LYPD8, REG4, APOA4, TCONS_00223467, and TCONS_00241897). Then, a two-step machine learning approach (RX) combination Random Forest and Extreme Gradient Boosting were performed, which identified 16 mRNAs and 17 lncRNAs as potential biomarkers, within which PPP2R3A and TCONS_00182693 were prioritized as key biomarkers involved in E. coli F17 infection. Furthermore, functional enrichment analysis showed that peroxisome proliferator-activated receptor (PPAR) pathway was significantly enriched in response to E. coli F17 infection. Our finding will help to improve the knowledge of the mechanisms underlying E. coli F17 infection and may provide novel targets for future treatment of E. coli F17 infection. Frontiers Media S.A. 2022-04-12 /pmc/articles/PMC9039264/ /pubmed/35498757 http://dx.doi.org/10.3389/fvets.2022.819917 Text en Copyright © 2022 Chen, Lv, Zhang, Hu, Cao, Ren, Getachew, Mwacharo, Haile and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Chen, Weihao
Lv, Xiaoyang
Zhang, Weibo
Hu, Tingyan
Cao, Xiukai
Ren, Ziming
Getachew, Tesfaye
Mwacharo, Joram M.
Haile, Aynalem
Sun, Wei
Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17
title Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17
title_full Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17
title_fullStr Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17
title_full_unstemmed Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17
title_short Insights Into Long Non-Coding RNA and mRNA Expression in the Jejunum of Lambs Challenged With Escherichia coli F17
title_sort insights into long non-coding rna and mrna expression in the jejunum of lambs challenged with escherichia coli f17
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039264/
https://www.ncbi.nlm.nih.gov/pubmed/35498757
http://dx.doi.org/10.3389/fvets.2022.819917
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