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Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation
Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals and recycling of gaseous and solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis of biocatalyst metabolism, and engineering of cell factories are advancing the widespre...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039284/ https://www.ncbi.nlm.nih.gov/pubmed/35497340 http://dx.doi.org/10.3389/fbioe.2022.879578 |
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author | de Lima, Lorena Azevedo Ingelman, Henri Brahmbhatt, Kush Reinmets, Kristina Barry, Craig Harris, Audrey Marcellin, Esteban Köpke, Michael Valgepea, Kaspar |
author_facet | de Lima, Lorena Azevedo Ingelman, Henri Brahmbhatt, Kush Reinmets, Kristina Barry, Craig Harris, Audrey Marcellin, Esteban Köpke, Michael Valgepea, Kaspar |
author_sort | de Lima, Lorena Azevedo |
collection | PubMed |
description | Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals and recycling of gaseous and solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis of biocatalyst metabolism, and engineering of cell factories are advancing the widespread deployment of the commercialised technology. Acetogens are particularly attractive biocatalysts but effects of the key physiological parameter–specific growth rate (μ)—on acetogen metabolism and the gas fermentation bioprocess have not been established yet. Here, we investigate the μ-dependent bioprocess performance of the model-acetogen Clostridium autoethanogenum in CO and syngas (CO + CO(2)+H(2)) grown chemostat cultures and assess systems-level metabolic responses using gas analysis, metabolomics, transcriptomics, and metabolic modelling. We were able to obtain steady-states up to μ ∼2.8 day(−1) (∼0.12 h(−1)) and show that faster growth supports both higher yields and productivities for reduced by-products ethanol and 2,3-butanediol. Transcriptomics data revealed differential expression of 1,337 genes with increasing μ and suggest that C. autoethanogenum uses transcriptional regulation to a large extent for facilitating faster growth. Metabolic modelling showed significantly increased fluxes for faster growing cells that were, however, not accompanied by gene expression changes in key catabolic pathways for CO and H(2) metabolism. Cells thus seem to maintain sufficient “baseline” gene expression to rapidly respond to CO and H(2) availability without delays to kick-start metabolism. Our work advances understanding of transcriptional regulation in acetogens and shows that faster growth of the biocatalyst improves the gas fermentation bioprocess. |
format | Online Article Text |
id | pubmed-9039284 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90392842022-04-27 Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation de Lima, Lorena Azevedo Ingelman, Henri Brahmbhatt, Kush Reinmets, Kristina Barry, Craig Harris, Audrey Marcellin, Esteban Köpke, Michael Valgepea, Kaspar Front Bioeng Biotechnol Bioengineering and Biotechnology Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals and recycling of gaseous and solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis of biocatalyst metabolism, and engineering of cell factories are advancing the widespread deployment of the commercialised technology. Acetogens are particularly attractive biocatalysts but effects of the key physiological parameter–specific growth rate (μ)—on acetogen metabolism and the gas fermentation bioprocess have not been established yet. Here, we investigate the μ-dependent bioprocess performance of the model-acetogen Clostridium autoethanogenum in CO and syngas (CO + CO(2)+H(2)) grown chemostat cultures and assess systems-level metabolic responses using gas analysis, metabolomics, transcriptomics, and metabolic modelling. We were able to obtain steady-states up to μ ∼2.8 day(−1) (∼0.12 h(−1)) and show that faster growth supports both higher yields and productivities for reduced by-products ethanol and 2,3-butanediol. Transcriptomics data revealed differential expression of 1,337 genes with increasing μ and suggest that C. autoethanogenum uses transcriptional regulation to a large extent for facilitating faster growth. Metabolic modelling showed significantly increased fluxes for faster growing cells that were, however, not accompanied by gene expression changes in key catabolic pathways for CO and H(2) metabolism. Cells thus seem to maintain sufficient “baseline” gene expression to rapidly respond to CO and H(2) availability without delays to kick-start metabolism. Our work advances understanding of transcriptional regulation in acetogens and shows that faster growth of the biocatalyst improves the gas fermentation bioprocess. Frontiers Media S.A. 2022-04-12 /pmc/articles/PMC9039284/ /pubmed/35497340 http://dx.doi.org/10.3389/fbioe.2022.879578 Text en Copyright © 2022 de Lima, Ingelman, Brahmbhatt, Reinmets, Barry, Harris, Marcellin, Köpke and Valgepea. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioengineering and Biotechnology de Lima, Lorena Azevedo Ingelman, Henri Brahmbhatt, Kush Reinmets, Kristina Barry, Craig Harris, Audrey Marcellin, Esteban Köpke, Michael Valgepea, Kaspar Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation |
title | Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation |
title_full | Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation |
title_fullStr | Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation |
title_full_unstemmed | Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation |
title_short | Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation |
title_sort | faster growth enhances low carbon fuel and chemical production through gas fermentation |
topic | Bioengineering and Biotechnology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039284/ https://www.ncbi.nlm.nih.gov/pubmed/35497340 http://dx.doi.org/10.3389/fbioe.2022.879578 |
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