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Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation

Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals and recycling of gaseous and solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis of biocatalyst metabolism, and engineering of cell factories are advancing the widespre...

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Autores principales: de Lima, Lorena Azevedo, Ingelman, Henri, Brahmbhatt, Kush, Reinmets, Kristina, Barry, Craig, Harris, Audrey, Marcellin, Esteban, Köpke, Michael, Valgepea, Kaspar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039284/
https://www.ncbi.nlm.nih.gov/pubmed/35497340
http://dx.doi.org/10.3389/fbioe.2022.879578
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author de Lima, Lorena Azevedo
Ingelman, Henri
Brahmbhatt, Kush
Reinmets, Kristina
Barry, Craig
Harris, Audrey
Marcellin, Esteban
Köpke, Michael
Valgepea, Kaspar
author_facet de Lima, Lorena Azevedo
Ingelman, Henri
Brahmbhatt, Kush
Reinmets, Kristina
Barry, Craig
Harris, Audrey
Marcellin, Esteban
Köpke, Michael
Valgepea, Kaspar
author_sort de Lima, Lorena Azevedo
collection PubMed
description Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals and recycling of gaseous and solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis of biocatalyst metabolism, and engineering of cell factories are advancing the widespread deployment of the commercialised technology. Acetogens are particularly attractive biocatalysts but effects of the key physiological parameter–specific growth rate (μ)—on acetogen metabolism and the gas fermentation bioprocess have not been established yet. Here, we investigate the μ-dependent bioprocess performance of the model-acetogen Clostridium autoethanogenum in CO and syngas (CO + CO(2)+H(2)) grown chemostat cultures and assess systems-level metabolic responses using gas analysis, metabolomics, transcriptomics, and metabolic modelling. We were able to obtain steady-states up to μ ∼2.8 day(−1) (∼0.12 h(−1)) and show that faster growth supports both higher yields and productivities for reduced by-products ethanol and 2,3-butanediol. Transcriptomics data revealed differential expression of 1,337 genes with increasing μ and suggest that C. autoethanogenum uses transcriptional regulation to a large extent for facilitating faster growth. Metabolic modelling showed significantly increased fluxes for faster growing cells that were, however, not accompanied by gene expression changes in key catabolic pathways for CO and H(2) metabolism. Cells thus seem to maintain sufficient “baseline” gene expression to rapidly respond to CO and H(2) availability without delays to kick-start metabolism. Our work advances understanding of transcriptional regulation in acetogens and shows that faster growth of the biocatalyst improves the gas fermentation bioprocess.
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spelling pubmed-90392842022-04-27 Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation de Lima, Lorena Azevedo Ingelman, Henri Brahmbhatt, Kush Reinmets, Kristina Barry, Craig Harris, Audrey Marcellin, Esteban Köpke, Michael Valgepea, Kaspar Front Bioeng Biotechnol Bioengineering and Biotechnology Gas fermentation offers both fossil carbon-free sustainable production of fuels and chemicals and recycling of gaseous and solid waste using gas-fermenting microbes. Bioprocess development, systems-level analysis of biocatalyst metabolism, and engineering of cell factories are advancing the widespread deployment of the commercialised technology. Acetogens are particularly attractive biocatalysts but effects of the key physiological parameter–specific growth rate (μ)—on acetogen metabolism and the gas fermentation bioprocess have not been established yet. Here, we investigate the μ-dependent bioprocess performance of the model-acetogen Clostridium autoethanogenum in CO and syngas (CO + CO(2)+H(2)) grown chemostat cultures and assess systems-level metabolic responses using gas analysis, metabolomics, transcriptomics, and metabolic modelling. We were able to obtain steady-states up to μ ∼2.8 day(−1) (∼0.12 h(−1)) and show that faster growth supports both higher yields and productivities for reduced by-products ethanol and 2,3-butanediol. Transcriptomics data revealed differential expression of 1,337 genes with increasing μ and suggest that C. autoethanogenum uses transcriptional regulation to a large extent for facilitating faster growth. Metabolic modelling showed significantly increased fluxes for faster growing cells that were, however, not accompanied by gene expression changes in key catabolic pathways for CO and H(2) metabolism. Cells thus seem to maintain sufficient “baseline” gene expression to rapidly respond to CO and H(2) availability without delays to kick-start metabolism. Our work advances understanding of transcriptional regulation in acetogens and shows that faster growth of the biocatalyst improves the gas fermentation bioprocess. Frontiers Media S.A. 2022-04-12 /pmc/articles/PMC9039284/ /pubmed/35497340 http://dx.doi.org/10.3389/fbioe.2022.879578 Text en Copyright © 2022 de Lima, Ingelman, Brahmbhatt, Reinmets, Barry, Harris, Marcellin, Köpke and Valgepea. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
de Lima, Lorena Azevedo
Ingelman, Henri
Brahmbhatt, Kush
Reinmets, Kristina
Barry, Craig
Harris, Audrey
Marcellin, Esteban
Köpke, Michael
Valgepea, Kaspar
Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation
title Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation
title_full Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation
title_fullStr Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation
title_full_unstemmed Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation
title_short Faster Growth Enhances Low Carbon Fuel and Chemical Production Through Gas Fermentation
title_sort faster growth enhances low carbon fuel and chemical production through gas fermentation
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039284/
https://www.ncbi.nlm.nih.gov/pubmed/35497340
http://dx.doi.org/10.3389/fbioe.2022.879578
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