Cargando…
Visualizing Cortical Development and Evolution: A Toolkit Update
Visualizing the process of neural circuit formation during neurogenesis, using genetically modified animals or somatic transgenesis of exogenous plasmids, has become a key to decipher cortical development and evolution. In contrast to the establishment of transgenic animals, the designing and prepar...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039325/ https://www.ncbi.nlm.nih.gov/pubmed/35495046 http://dx.doi.org/10.3389/fnins.2022.876406 |
_version_ | 1784694103022239744 |
---|---|
author | Kumamoto, Takuma Ohtaka-Maruyama, Chiaki |
author_facet | Kumamoto, Takuma Ohtaka-Maruyama, Chiaki |
author_sort | Kumamoto, Takuma |
collection | PubMed |
description | Visualizing the process of neural circuit formation during neurogenesis, using genetically modified animals or somatic transgenesis of exogenous plasmids, has become a key to decipher cortical development and evolution. In contrast to the establishment of transgenic animals, the designing and preparation of genes of interest into plasmids are simple and easy, dispensing with time-consuming germline modifications. These advantages have led to neuron labeling based on somatic transgenesis. In particular, mammalian expression plasmid, CRISPR-Cas9, and DNA transposon systems, have become widely used for neuronal visualization and functional analysis related to lineage labeling during cortical development. In this review, we discuss the advantages and limitations of these recently developed techniques. |
format | Online Article Text |
id | pubmed-9039325 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90393252022-04-27 Visualizing Cortical Development and Evolution: A Toolkit Update Kumamoto, Takuma Ohtaka-Maruyama, Chiaki Front Neurosci Neuroscience Visualizing the process of neural circuit formation during neurogenesis, using genetically modified animals or somatic transgenesis of exogenous plasmids, has become a key to decipher cortical development and evolution. In contrast to the establishment of transgenic animals, the designing and preparation of genes of interest into plasmids are simple and easy, dispensing with time-consuming germline modifications. These advantages have led to neuron labeling based on somatic transgenesis. In particular, mammalian expression plasmid, CRISPR-Cas9, and DNA transposon systems, have become widely used for neuronal visualization and functional analysis related to lineage labeling during cortical development. In this review, we discuss the advantages and limitations of these recently developed techniques. Frontiers Media S.A. 2022-04-12 /pmc/articles/PMC9039325/ /pubmed/35495046 http://dx.doi.org/10.3389/fnins.2022.876406 Text en Copyright © 2022 Kumamoto and Ohtaka-Maruyama. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Kumamoto, Takuma Ohtaka-Maruyama, Chiaki Visualizing Cortical Development and Evolution: A Toolkit Update |
title | Visualizing Cortical Development and Evolution: A Toolkit Update |
title_full | Visualizing Cortical Development and Evolution: A Toolkit Update |
title_fullStr | Visualizing Cortical Development and Evolution: A Toolkit Update |
title_full_unstemmed | Visualizing Cortical Development and Evolution: A Toolkit Update |
title_short | Visualizing Cortical Development and Evolution: A Toolkit Update |
title_sort | visualizing cortical development and evolution: a toolkit update |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039325/ https://www.ncbi.nlm.nih.gov/pubmed/35495046 http://dx.doi.org/10.3389/fnins.2022.876406 |
work_keys_str_mv | AT kumamototakuma visualizingcorticaldevelopmentandevolutionatoolkitupdate AT ohtakamaruyamachiaki visualizingcorticaldevelopmentandevolutionatoolkitupdate |