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Visualizing Cortical Development and Evolution: A Toolkit Update

Visualizing the process of neural circuit formation during neurogenesis, using genetically modified animals or somatic transgenesis of exogenous plasmids, has become a key to decipher cortical development and evolution. In contrast to the establishment of transgenic animals, the designing and prepar...

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Detalles Bibliográficos
Autores principales: Kumamoto, Takuma, Ohtaka-Maruyama, Chiaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039325/
https://www.ncbi.nlm.nih.gov/pubmed/35495046
http://dx.doi.org/10.3389/fnins.2022.876406
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author Kumamoto, Takuma
Ohtaka-Maruyama, Chiaki
author_facet Kumamoto, Takuma
Ohtaka-Maruyama, Chiaki
author_sort Kumamoto, Takuma
collection PubMed
description Visualizing the process of neural circuit formation during neurogenesis, using genetically modified animals or somatic transgenesis of exogenous plasmids, has become a key to decipher cortical development and evolution. In contrast to the establishment of transgenic animals, the designing and preparation of genes of interest into plasmids are simple and easy, dispensing with time-consuming germline modifications. These advantages have led to neuron labeling based on somatic transgenesis. In particular, mammalian expression plasmid, CRISPR-Cas9, and DNA transposon systems, have become widely used for neuronal visualization and functional analysis related to lineage labeling during cortical development. In this review, we discuss the advantages and limitations of these recently developed techniques.
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spelling pubmed-90393252022-04-27 Visualizing Cortical Development and Evolution: A Toolkit Update Kumamoto, Takuma Ohtaka-Maruyama, Chiaki Front Neurosci Neuroscience Visualizing the process of neural circuit formation during neurogenesis, using genetically modified animals or somatic transgenesis of exogenous plasmids, has become a key to decipher cortical development and evolution. In contrast to the establishment of transgenic animals, the designing and preparation of genes of interest into plasmids are simple and easy, dispensing with time-consuming germline modifications. These advantages have led to neuron labeling based on somatic transgenesis. In particular, mammalian expression plasmid, CRISPR-Cas9, and DNA transposon systems, have become widely used for neuronal visualization and functional analysis related to lineage labeling during cortical development. In this review, we discuss the advantages and limitations of these recently developed techniques. Frontiers Media S.A. 2022-04-12 /pmc/articles/PMC9039325/ /pubmed/35495046 http://dx.doi.org/10.3389/fnins.2022.876406 Text en Copyright © 2022 Kumamoto and Ohtaka-Maruyama. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Kumamoto, Takuma
Ohtaka-Maruyama, Chiaki
Visualizing Cortical Development and Evolution: A Toolkit Update
title Visualizing Cortical Development and Evolution: A Toolkit Update
title_full Visualizing Cortical Development and Evolution: A Toolkit Update
title_fullStr Visualizing Cortical Development and Evolution: A Toolkit Update
title_full_unstemmed Visualizing Cortical Development and Evolution: A Toolkit Update
title_short Visualizing Cortical Development and Evolution: A Toolkit Update
title_sort visualizing cortical development and evolution: a toolkit update
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039325/
https://www.ncbi.nlm.nih.gov/pubmed/35495046
http://dx.doi.org/10.3389/fnins.2022.876406
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