Wristwatch PCR: A Versatile and Efficient Genome Walking Strategy

Genome walking is a method used to retrieve unknown flanking DNA. Here, we reported wristwatch (WW) PCR, an efficient genome walking technique mediated by WW primers (WWPs). WWPs feature 5′- and 3′-overlap and a heterologous interval. Therefore, a wristwatch-like structure can be formed between WWPs...

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Detalles Bibliográficos
Autores principales: Wang, Lingqin, Jia, Mengya, Li, Zhaoqin, Liu, Xiaohua, Sun, Tianyi, Pei, Jinfeng, Wei, Cheng, Lin, Zhiyu, Li, Haixing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039356/
https://www.ncbi.nlm.nih.gov/pubmed/35497369
http://dx.doi.org/10.3389/fbioe.2022.792848

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