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Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus

The leucine-rich repeat receptor-like protein kinase (LRR-RLK) family represents the largest group of RLKs in plants and plays vital roles in plant growth, development and the responses to environmental stress. Although LRR-RLK families have been identified in many species, they have not yet been re...

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Autores principales: Song, Min, Linghu, Bin, Huang, Shuhua, Li, Fang, An, Ran, Xie, Changgen, Zhu, Yantao, Hu, Shengwu, Mu, Jianxin, Zhang, Yanfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039726/
https://www.ncbi.nlm.nih.gov/pubmed/35498707
http://dx.doi.org/10.3389/fpls.2022.865132
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author Song, Min
Linghu, Bin
Huang, Shuhua
Li, Fang
An, Ran
Xie, Changgen
Zhu, Yantao
Hu, Shengwu
Mu, Jianxin
Zhang, Yanfeng
author_facet Song, Min
Linghu, Bin
Huang, Shuhua
Li, Fang
An, Ran
Xie, Changgen
Zhu, Yantao
Hu, Shengwu
Mu, Jianxin
Zhang, Yanfeng
author_sort Song, Min
collection PubMed
description The leucine-rich repeat receptor-like protein kinase (LRR-RLK) family represents the largest group of RLKs in plants and plays vital roles in plant growth, development and the responses to environmental stress. Although LRR-RLK families have been identified in many species, they have not yet been reported in B. napus. In this study, a total of 444 BnLRR-RLK genes were identified in the genome of Brassica napus cultivar “Zhongshuang 11” (ZS11), and classified into 22 subfamilies based on phylogenetic relationships and genome-wide analyses. Conserved motifs and gene structures were shared within but not between subfamilies. The 444 BnLRR-RLK genes were asymmetrically distributed on 19 chromosomes and exhibited specific expression profiles in different tissues and in response to stress. We identified six BnBRI1 homologs and obtained partial knockouts via CRISPR/Cas9 technology, generating semi-dwarf lines without decreased yield compared with controls. This study provides comprehensive insight of the LRR-RLK family in B. napus. Additionally, the semi-dwarf lines expand the “ideotype” germplasm resources and accelerate the breeding process for B. napus.
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spelling pubmed-90397262022-04-27 Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus Song, Min Linghu, Bin Huang, Shuhua Li, Fang An, Ran Xie, Changgen Zhu, Yantao Hu, Shengwu Mu, Jianxin Zhang, Yanfeng Front Plant Sci Plant Science The leucine-rich repeat receptor-like protein kinase (LRR-RLK) family represents the largest group of RLKs in plants and plays vital roles in plant growth, development and the responses to environmental stress. Although LRR-RLK families have been identified in many species, they have not yet been reported in B. napus. In this study, a total of 444 BnLRR-RLK genes were identified in the genome of Brassica napus cultivar “Zhongshuang 11” (ZS11), and classified into 22 subfamilies based on phylogenetic relationships and genome-wide analyses. Conserved motifs and gene structures were shared within but not between subfamilies. The 444 BnLRR-RLK genes were asymmetrically distributed on 19 chromosomes and exhibited specific expression profiles in different tissues and in response to stress. We identified six BnBRI1 homologs and obtained partial knockouts via CRISPR/Cas9 technology, generating semi-dwarf lines without decreased yield compared with controls. This study provides comprehensive insight of the LRR-RLK family in B. napus. Additionally, the semi-dwarf lines expand the “ideotype” germplasm resources and accelerate the breeding process for B. napus. Frontiers Media S.A. 2022-04-12 /pmc/articles/PMC9039726/ /pubmed/35498707 http://dx.doi.org/10.3389/fpls.2022.865132 Text en Copyright © 2022 Song, Linghu, Huang, Li, An, Xie, Zhu, Hu, Mu and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Song, Min
Linghu, Bin
Huang, Shuhua
Li, Fang
An, Ran
Xie, Changgen
Zhu, Yantao
Hu, Shengwu
Mu, Jianxin
Zhang, Yanfeng
Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus
title Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus
title_full Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus
title_fullStr Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus
title_full_unstemmed Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus
title_short Genome-Wide Survey of Leucine-Rich Repeat Receptor-Like Protein Kinase Genes and CRISPR/Cas9-Targeted Mutagenesis BnBRI1 in Brassica napus
title_sort genome-wide survey of leucine-rich repeat receptor-like protein kinase genes and crispr/cas9-targeted mutagenesis bnbri1 in brassica napus
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9039726/
https://www.ncbi.nlm.nih.gov/pubmed/35498707
http://dx.doi.org/10.3389/fpls.2022.865132
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