CRISPR-Cas9 Targeting of Hepatitis B Virus Covalently Closed Circular DNA Generates Transcriptionally Active Episomal Variants

Chronic hepatitis B virus (HBV) infection persists due to the lack of therapies that effectively target the HBV covalently closed circular DNA (cccDNA). We used HBV-specific guide RNAs (gRNAs) and CRISPR-Cas9 and determined the fate of cccDNA after gene editing. We set up a ribonucleoprotein (RNP) d...

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Detalles Bibliográficos
Autores principales: Martinez, Maria Guadalupe, Combe, Emmanuel, Inchauspe, Aurore, Mangeot, Philippe Emmanuel, Delberghe, Elodie, Chapus, Fleur, Neveu, Gregory, Alam, Antoine, Carter, Kara, Testoni, Barbara, Zoulim, Fabien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040760/
https://www.ncbi.nlm.nih.gov/pubmed/35389262
http://dx.doi.org/10.1128/mbio.02888-21

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9040760/
https://www.ncbi.nlm.nih.gov/pubmed/35389262
http://dx.doi.org/10.1128/mbio.02888-21

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