Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst

Immobilized whole-cells of Pichia pastoris harboring recombinant d-lactonase were entrapped in calcium alginate gels and used as an efficient biocatalyst for catalytic kinetic resolution of d,l-pantolactone. The immobilized whole-cell biocatalyst exhibited good catalytic stability, which was applied...

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Autores principales: Zhang, Qiu-Hua, Yang, Liu, Tang, Yi-Bin, Huang, Liu-Nv, Luo, Wen-Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041136/
https://www.ncbi.nlm.nih.gov/pubmed/35480294
http://dx.doi.org/10.1039/d1ra05708a
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author Zhang, Qiu-Hua
Yang, Liu
Tang, Yi-Bin
Huang, Liu-Nv
Luo, Wen-Fang
author_facet Zhang, Qiu-Hua
Yang, Liu
Tang, Yi-Bin
Huang, Liu-Nv
Luo, Wen-Fang
author_sort Zhang, Qiu-Hua
collection PubMed
description Immobilized whole-cells of Pichia pastoris harboring recombinant d-lactonase were entrapped in calcium alginate gels and used as an efficient biocatalyst for catalytic kinetic resolution of d,l-pantolactone. The immobilized whole-cell biocatalyst exhibited good catalytic stability, which was applied for stereospecific hydrolysis of d-pantolactone for up to 56 repeated batch reactions without obvious loss in the catalytic activity and enantioselectivity.
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spelling pubmed-90411362022-04-26 Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst Zhang, Qiu-Hua Yang, Liu Tang, Yi-Bin Huang, Liu-Nv Luo, Wen-Fang RSC Adv Chemistry Immobilized whole-cells of Pichia pastoris harboring recombinant d-lactonase were entrapped in calcium alginate gels and used as an efficient biocatalyst for catalytic kinetic resolution of d,l-pantolactone. The immobilized whole-cell biocatalyst exhibited good catalytic stability, which was applied for stereospecific hydrolysis of d-pantolactone for up to 56 repeated batch reactions without obvious loss in the catalytic activity and enantioselectivity. The Royal Society of Chemistry 2021-09-13 /pmc/articles/PMC9041136/ /pubmed/35480294 http://dx.doi.org/10.1039/d1ra05708a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zhang, Qiu-Hua
Yang, Liu
Tang, Yi-Bin
Huang, Liu-Nv
Luo, Wen-Fang
Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst
title Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst
title_full Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst
title_fullStr Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst
title_full_unstemmed Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst
title_short Industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst
title_sort industrial kinetic resolution of d,l-pantolactone by an immobilized whole-cell biocatalyst
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041136/
https://www.ncbi.nlm.nih.gov/pubmed/35480294
http://dx.doi.org/10.1039/d1ra05708a
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