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Design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers
The potential of peripheral nerves to regenerate under the effect of axial tensile forces was not previously extensively explored due to the lack of capabilities of translating ex vivo axonal stretch-growth to in vivo studies, until the development of a nerve stretcher. The nerve stretcher, which we...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041162/ https://www.ncbi.nlm.nih.gov/pubmed/35495205 http://dx.doi.org/10.1016/j.ohx.2020.e00093 |
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author | Sahar, Muhammad Sana Ullah Barton, Matthew Tansley, Geoffrey |
author_facet | Sahar, Muhammad Sana Ullah Barton, Matthew Tansley, Geoffrey |
author_sort | Sahar, Muhammad Sana Ullah |
collection | PubMed |
description | The potential of peripheral nerves to regenerate under the effect of axial tensile forces was not previously extensively explored due to the lack of capabilities of translating ex vivo axonal stretch-growth to in vivo studies, until the development of a nerve stretcher. The nerve stretcher, which we have designed and manufactured recently, is a device that uses a controlled amount of axial tensile force (vacuum/negative gauge pressure) applied directly to a sectioned peripheral nerve in vivo to expedite nerve regrowth rate. Using this platform, a series of experiments was carried out to observe the effect of in vivo axial stretch on axonal lengthening. During these experiments, a few challenges necessitated redesigning the device like a sudden loss of stretching force due to vacuum leakage, erroneous feedback from vacuum sensor due to sensor drift, and inability to control and operate the device remotely. Here we present an improved design of the nerve stretcher along with its integration with a state-of-the-art online vacuum monitoring facility to control, collect, process, and visualize negative gauge pressure data in real-time. |
format | Online Article Text |
id | pubmed-9041162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-90411622022-04-27 Design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers Sahar, Muhammad Sana Ullah Barton, Matthew Tansley, Geoffrey HardwareX Hardware Article The potential of peripheral nerves to regenerate under the effect of axial tensile forces was not previously extensively explored due to the lack of capabilities of translating ex vivo axonal stretch-growth to in vivo studies, until the development of a nerve stretcher. The nerve stretcher, which we have designed and manufactured recently, is a device that uses a controlled amount of axial tensile force (vacuum/negative gauge pressure) applied directly to a sectioned peripheral nerve in vivo to expedite nerve regrowth rate. Using this platform, a series of experiments was carried out to observe the effect of in vivo axial stretch on axonal lengthening. During these experiments, a few challenges necessitated redesigning the device like a sudden loss of stretching force due to vacuum leakage, erroneous feedback from vacuum sensor due to sensor drift, and inability to control and operate the device remotely. Here we present an improved design of the nerve stretcher along with its integration with a state-of-the-art online vacuum monitoring facility to control, collect, process, and visualize negative gauge pressure data in real-time. Elsevier 2020-02-07 /pmc/articles/PMC9041162/ /pubmed/35495205 http://dx.doi.org/10.1016/j.ohx.2020.e00093 Text en © 2020 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Hardware Article Sahar, Muhammad Sana Ullah Barton, Matthew Tansley, Geoffrey Design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers |
title | Design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers |
title_full | Design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers |
title_fullStr | Design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers |
title_full_unstemmed | Design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers |
title_short | Design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers |
title_sort | design and fabrication of a nerve-stretching device for in vivo mechanotransduction of peripheral nerve fibers |
topic | Hardware Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9041162/ https://www.ncbi.nlm.nih.gov/pubmed/35495205 http://dx.doi.org/10.1016/j.ohx.2020.e00093 |
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