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Ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from Carapa guianensis seeds and the application of lipidomics using GC/MS

This study describes the optimized stages of lipid extraction assisted by ultrasound to increase the concentrations of limonoids and steroids from andiroba seeds, identified as Carapa guianensis Aublet, and the lipidome analyzed by TLC and GC/MS. After boiling and peeling, crushed seeds were subject...

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Autores principales: Silva dos Reis, André, Santos, Alberdan Silva, Francisco de Carvalho Gonçalves, José
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042240/
https://www.ncbi.nlm.nih.gov/pubmed/35493601
http://dx.doi.org/10.1039/d1ra04776k
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author Silva dos Reis, André
Santos, Alberdan Silva
Francisco de Carvalho Gonçalves, José
author_facet Silva dos Reis, André
Santos, Alberdan Silva
Francisco de Carvalho Gonçalves, José
author_sort Silva dos Reis, André
collection PubMed
description This study describes the optimized stages of lipid extraction assisted by ultrasound to increase the concentrations of limonoids and steroids from andiroba seeds, identified as Carapa guianensis Aublet, and the lipidome analyzed by TLC and GC/MS. After boiling and peeling, crushed seeds were subjected to extractions with n-hexane (G1), acetone (G2), and methanol (G3) in an ultrasonic bath for 30 minutes at 50 °C. These extracts were analyzed by analytical TLC; aliquots of each extract and a marketable oil, used as a reference, were transesterified followed by silylation with BSTFA + 1% TMCS, and analyzed by GC/MS. The reference oil and the biomass of the seeds did not present significant differences in the profiles of free fatty acids (FFA). However, differences were observed in the profiles of tetranortriterpenoids (TnTT) (limonoids) in the seeds. Afterward, the analysis of the extracts G1, G2, and G3 detected free steroids: campesterol, stigmasterol, and β-sitosterol; and limonoids: 7-deacetyl-7-oxogedunin, 6α-acetoxy-gedunin, deacetylgedunin, and epoxyacetylgedunin. The use of lipidomic techniques associated with ultrasound-assisted extraction was applied for the first time to enhance the triterpenoids and steroids, mainly in G3, describing a faster and more economical process, and allowing a one-step lipidome analysis of the andiroba seeds.
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spelling pubmed-90422402022-04-28 Ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from Carapa guianensis seeds and the application of lipidomics using GC/MS Silva dos Reis, André Santos, Alberdan Silva Francisco de Carvalho Gonçalves, José RSC Adv Chemistry This study describes the optimized stages of lipid extraction assisted by ultrasound to increase the concentrations of limonoids and steroids from andiroba seeds, identified as Carapa guianensis Aublet, and the lipidome analyzed by TLC and GC/MS. After boiling and peeling, crushed seeds were subjected to extractions with n-hexane (G1), acetone (G2), and methanol (G3) in an ultrasonic bath for 30 minutes at 50 °C. These extracts were analyzed by analytical TLC; aliquots of each extract and a marketable oil, used as a reference, were transesterified followed by silylation with BSTFA + 1% TMCS, and analyzed by GC/MS. The reference oil and the biomass of the seeds did not present significant differences in the profiles of free fatty acids (FFA). However, differences were observed in the profiles of tetranortriterpenoids (TnTT) (limonoids) in the seeds. Afterward, the analysis of the extracts G1, G2, and G3 detected free steroids: campesterol, stigmasterol, and β-sitosterol; and limonoids: 7-deacetyl-7-oxogedunin, 6α-acetoxy-gedunin, deacetylgedunin, and epoxyacetylgedunin. The use of lipidomic techniques associated with ultrasound-assisted extraction was applied for the first time to enhance the triterpenoids and steroids, mainly in G3, describing a faster and more economical process, and allowing a one-step lipidome analysis of the andiroba seeds. The Royal Society of Chemistry 2021-10-08 /pmc/articles/PMC9042240/ /pubmed/35493601 http://dx.doi.org/10.1039/d1ra04776k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Silva dos Reis, André
Santos, Alberdan Silva
Francisco de Carvalho Gonçalves, José
Ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from Carapa guianensis seeds and the application of lipidomics using GC/MS
title Ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from Carapa guianensis seeds and the application of lipidomics using GC/MS
title_full Ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from Carapa guianensis seeds and the application of lipidomics using GC/MS
title_fullStr Ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from Carapa guianensis seeds and the application of lipidomics using GC/MS
title_full_unstemmed Ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from Carapa guianensis seeds and the application of lipidomics using GC/MS
title_short Ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from Carapa guianensis seeds and the application of lipidomics using GC/MS
title_sort ultrasound-assisted lipid extractions, enriched with sterols and tetranortriterpenoids, from carapa guianensis seeds and the application of lipidomics using gc/ms
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042240/
https://www.ncbi.nlm.nih.gov/pubmed/35493601
http://dx.doi.org/10.1039/d1ra04776k
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