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A highly selective quinolizinium-based fluorescent probe for cysteine detection

A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (...

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Detalles Bibliográficos
Autores principales: O, Wa-Yi, Chan, Wing-Cheung, Xu, Caifeng, Deng, Jie-Ren, Ko, Ben Chi-Bun, Wong, Man-Kin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042279/
https://www.ncbi.nlm.nih.gov/pubmed/35497514
http://dx.doi.org/10.1039/d1ra06104f
Descripción
Sumario:A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96–109%).