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A highly selective quinolizinium-based fluorescent probe for cysteine detection
A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
The Royal Society of Chemistry
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042279/ https://www.ncbi.nlm.nih.gov/pubmed/35497514 http://dx.doi.org/10.1039/d1ra06104f |
| _version_ | 1784694629402148864 |
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| author | O, Wa-Yi Chan, Wing-Cheung Xu, Caifeng Deng, Jie-Ren Ko, Ben Chi-Bun Wong, Man-Kin |
| author_facet | O, Wa-Yi Chan, Wing-Cheung Xu, Caifeng Deng, Jie-Ren Ko, Ben Chi-Bun Wong, Man-Kin |
| author_sort | O, Wa-Yi |
| collection | PubMed |
| description | A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96–109%). |
| format | Online Article Text |
| id | pubmed-9042279 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | The Royal Society of Chemistry |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-90422792022-04-28 A highly selective quinolizinium-based fluorescent probe for cysteine detection O, Wa-Yi Chan, Wing-Cheung Xu, Caifeng Deng, Jie-Ren Ko, Ben Chi-Bun Wong, Man-Kin RSC Adv Chemistry A novel fluorescent quinolizinium-based turn-off probe has been developed for selective detection of cysteine. The probe showed high selectivity and sensitivity towards cysteine over other amino acids including the similarly structured homocysteine and glutathione with a detection limit of 0.18 μM (S/N = 3). It was successfully applied to cysteine detection in living cells with low cytotoxicity and quantitative analysis of spiked mouse serum samples with moderate to good recovery (96–109%). The Royal Society of Chemistry 2021-10-11 /pmc/articles/PMC9042279/ /pubmed/35497514 http://dx.doi.org/10.1039/d1ra06104f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
| spellingShingle | Chemistry O, Wa-Yi Chan, Wing-Cheung Xu, Caifeng Deng, Jie-Ren Ko, Ben Chi-Bun Wong, Man-Kin A highly selective quinolizinium-based fluorescent probe for cysteine detection |
| title | A highly selective quinolizinium-based fluorescent probe for cysteine detection |
| title_full | A highly selective quinolizinium-based fluorescent probe for cysteine detection |
| title_fullStr | A highly selective quinolizinium-based fluorescent probe for cysteine detection |
| title_full_unstemmed | A highly selective quinolizinium-based fluorescent probe for cysteine detection |
| title_short | A highly selective quinolizinium-based fluorescent probe for cysteine detection |
| title_sort | highly selective quinolizinium-based fluorescent probe for cysteine detection |
| topic | Chemistry |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042279/ https://www.ncbi.nlm.nih.gov/pubmed/35497514 http://dx.doi.org/10.1039/d1ra06104f |
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