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Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects—An In Vitro Study
OBJECTIVE: The aim of the study was to characterize and evaluate the stability, antimicrobial activity, cytotoxicity, and remineralizing effects of silver nanoparticles and fluoride anticaries agent (AgF) on staining dental enamel. MATERIALS AND METHODS: An experimental AgF solution was prepared and...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042602/ https://www.ncbi.nlm.nih.gov/pubmed/35497178 http://dx.doi.org/10.1155/2022/9483589 |
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author | Favaro, Jaqueline Costa Detomini, Tiago Roberto Maia, Luciana Prado Poli, Regina Célia Guiraldo, Ricardo Danil Lopes, Murilo Baena Berger, Sandrine Bittencourt |
author_facet | Favaro, Jaqueline Costa Detomini, Tiago Roberto Maia, Luciana Prado Poli, Regina Célia Guiraldo, Ricardo Danil Lopes, Murilo Baena Berger, Sandrine Bittencourt |
author_sort | Favaro, Jaqueline Costa |
collection | PubMed |
description | OBJECTIVE: The aim of the study was to characterize and evaluate the stability, antimicrobial activity, cytotoxicity, and remineralizing effects of silver nanoparticles and fluoride anticaries agent (AgF) on staining dental enamel. MATERIALS AND METHODS: An experimental AgF solution was prepared and compared to silver diamine fluoride (SDF). First, the AgF was characterized and the stability was evaluated by transmission electron microscopy (TEM). Streptococcus mutans, Enterococcus faecalis, and Escherichia coli strains were used to evaluate the minimum inhibitory and bactericidal concentration and cytotoxicity performed using L929 fibroblastic cells by MTT test. Caries-like lesions induced by pH-cycling in human enamel were obtained, and then, the superficial microhardness, cross-sectional microhardness (CSMH), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS) were performed. Photographic images were taken to analyze the enamel staining. RESULTS: The AgF showed stableness in long term with bacteriostatic and bactericidal actions without cytotoxicity. Enamel remineralization, in surface and in depth (CSMH), was observed when the AgF was used, and it was similar to SDF. SEM showed enamel precipitation, and EDS observed the presence of P, Ca, Au, Ag, and Cl elements. Contrary to SDF, AgF did not stain the enamel. CONCLUSION: The nano silver fluoride anticaries agent tested presented long-term stability, superficial and in-depth remineralizing capacity with antimicrobial potential and biocompatibility and did not stain the enamel. |
format | Online Article Text |
id | pubmed-9042602 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-90426022022-04-27 Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects—An In Vitro Study Favaro, Jaqueline Costa Detomini, Tiago Roberto Maia, Luciana Prado Poli, Regina Célia Guiraldo, Ricardo Danil Lopes, Murilo Baena Berger, Sandrine Bittencourt Int J Dent Research Article OBJECTIVE: The aim of the study was to characterize and evaluate the stability, antimicrobial activity, cytotoxicity, and remineralizing effects of silver nanoparticles and fluoride anticaries agent (AgF) on staining dental enamel. MATERIALS AND METHODS: An experimental AgF solution was prepared and compared to silver diamine fluoride (SDF). First, the AgF was characterized and the stability was evaluated by transmission electron microscopy (TEM). Streptococcus mutans, Enterococcus faecalis, and Escherichia coli strains were used to evaluate the minimum inhibitory and bactericidal concentration and cytotoxicity performed using L929 fibroblastic cells by MTT test. Caries-like lesions induced by pH-cycling in human enamel were obtained, and then, the superficial microhardness, cross-sectional microhardness (CSMH), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDS) were performed. Photographic images were taken to analyze the enamel staining. RESULTS: The AgF showed stableness in long term with bacteriostatic and bactericidal actions without cytotoxicity. Enamel remineralization, in surface and in depth (CSMH), was observed when the AgF was used, and it was similar to SDF. SEM showed enamel precipitation, and EDS observed the presence of P, Ca, Au, Ag, and Cl elements. Contrary to SDF, AgF did not stain the enamel. CONCLUSION: The nano silver fluoride anticaries agent tested presented long-term stability, superficial and in-depth remineralizing capacity with antimicrobial potential and biocompatibility and did not stain the enamel. Hindawi 2022-04-19 /pmc/articles/PMC9042602/ /pubmed/35497178 http://dx.doi.org/10.1155/2022/9483589 Text en Copyright © 2022 Jaqueline Costa Favaro et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Favaro, Jaqueline Costa Detomini, Tiago Roberto Maia, Luciana Prado Poli, Regina Célia Guiraldo, Ricardo Danil Lopes, Murilo Baena Berger, Sandrine Bittencourt Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects—An In Vitro Study |
title | Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects—An In Vitro Study |
title_full | Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects—An In Vitro Study |
title_fullStr | Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects—An In Vitro Study |
title_full_unstemmed | Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects—An In Vitro Study |
title_short | Anticaries Agent Based on Silver Nanoparticles and Fluoride: Characterization and Biological and Remineralizing Effects—An In Vitro Study |
title_sort | anticaries agent based on silver nanoparticles and fluoride: characterization and biological and remineralizing effects—an in vitro study |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042602/ https://www.ncbi.nlm.nih.gov/pubmed/35497178 http://dx.doi.org/10.1155/2022/9483589 |
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