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Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib
To study the mechanism of circular ribonucleic acid (RNA) circHIPK3 involved in the resistance of lung cancer cells to gefitinib, 110 patients with lung cancer were recruited as the research objects, and the tumor tissue and para-cancerous tissue of each patient's surgical specimens were collec...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042616/ https://www.ncbi.nlm.nih.gov/pubmed/35496045 http://dx.doi.org/10.1155/2022/4541918 |
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author | Zhao, Yi Zhang, Caiming Tang, Haibo Wu, Xiaomin Qi, Qiugan |
author_facet | Zhao, Yi Zhang, Caiming Tang, Haibo Wu, Xiaomin Qi, Qiugan |
author_sort | Zhao, Yi |
collection | PubMed |
description | To study the mechanism of circular ribonucleic acid (RNA) circHIPK3 involved in the resistance of lung cancer cells to gefitinib, 110 patients with lung cancer were recruited as the research objects, and the tumor tissue and para-cancerous tissue of each patient's surgical specimens were collected and paraffinized to detect the expression of circHIPK3 in different tissues. Gefitinib drug-resistant cell line of lung cancer was constructed with gefitinib to detect cell apoptosis under different conditions. As a result, the relative expression of circHIPK3 in patients with tumor diameter no less than 3 cm was dramatically inferior to that in patients with tumor diameter less than 3 cm (P < 0.05). The relative expression of circHIPK3 in patients with TNM stage II/III was dramatically inferior to that in patients with tumor, node, and metastasis (TNM) stage I (P < 0.05). Expression of circHIPK3 in patients with lymph node metastasis was dramatically inferior to that in patients without lymph node metastasis (P < 0.05). Of the lung cancer tissues of patients with different TNM stages, only six patients had high expression, and the remaining 104 patients had low expression. Moreover, electrophoresis revealed that circHIPK3 can only be amplified in cDNA, but not in gDNA. Gefitinib-mediated apoptosis rate of lung cancer drug-resistant cell lines decreased notably. In summary, the circular RNA circHIPK3 may have a notably low expression in lung cancer tissues, whose low expression had a certain enhancement effect on the drug resistance of lung adenocarcinoma cells to gefitinib. |
format | Online Article Text |
id | pubmed-9042616 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-90426162022-04-27 Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib Zhao, Yi Zhang, Caiming Tang, Haibo Wu, Xiaomin Qi, Qiugan Biomed Res Int Research Article To study the mechanism of circular ribonucleic acid (RNA) circHIPK3 involved in the resistance of lung cancer cells to gefitinib, 110 patients with lung cancer were recruited as the research objects, and the tumor tissue and para-cancerous tissue of each patient's surgical specimens were collected and paraffinized to detect the expression of circHIPK3 in different tissues. Gefitinib drug-resistant cell line of lung cancer was constructed with gefitinib to detect cell apoptosis under different conditions. As a result, the relative expression of circHIPK3 in patients with tumor diameter no less than 3 cm was dramatically inferior to that in patients with tumor diameter less than 3 cm (P < 0.05). The relative expression of circHIPK3 in patients with TNM stage II/III was dramatically inferior to that in patients with tumor, node, and metastasis (TNM) stage I (P < 0.05). Expression of circHIPK3 in patients with lymph node metastasis was dramatically inferior to that in patients without lymph node metastasis (P < 0.05). Of the lung cancer tissues of patients with different TNM stages, only six patients had high expression, and the remaining 104 patients had low expression. Moreover, electrophoresis revealed that circHIPK3 can only be amplified in cDNA, but not in gDNA. Gefitinib-mediated apoptosis rate of lung cancer drug-resistant cell lines decreased notably. In summary, the circular RNA circHIPK3 may have a notably low expression in lung cancer tissues, whose low expression had a certain enhancement effect on the drug resistance of lung adenocarcinoma cells to gefitinib. Hindawi 2022-04-19 /pmc/articles/PMC9042616/ /pubmed/35496045 http://dx.doi.org/10.1155/2022/4541918 Text en Copyright © 2022 Yi Zhao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhao, Yi Zhang, Caiming Tang, Haibo Wu, Xiaomin Qi, Qiugan Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib |
title | Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib |
title_full | Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib |
title_fullStr | Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib |
title_full_unstemmed | Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib |
title_short | Mechanism of RNA circHIPK3 Involved in Resistance of Lung Cancer Cells to Gefitinib |
title_sort | mechanism of rna circhipk3 involved in resistance of lung cancer cells to gefitinib |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042616/ https://www.ncbi.nlm.nih.gov/pubmed/35496045 http://dx.doi.org/10.1155/2022/4541918 |
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