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Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection
Fetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042633/ https://www.ncbi.nlm.nih.gov/pubmed/35498397 http://dx.doi.org/10.1155/2022/6082140 |
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author | Mahjabeen, Faria Yu, Jieh-Juen Chambers, James P. Gupta, Rishein Arulanandam, Bernard P. |
author_facet | Mahjabeen, Faria Yu, Jieh-Juen Chambers, James P. Gupta, Rishein Arulanandam, Bernard P. |
author_sort | Mahjabeen, Faria |
collection | PubMed |
description | Fetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased 46%, while INF-γ increased 44%, consistent with a negative regulatory role of fetuin-A in inflammation. Importantly, the observed increased IFN-γ production was abrogated in fetuin-A-deficient AHSG mice suggesting that IFN-γ induction following Cm infection is fetuin-A dependent. Assessment of expression of genes associated with inflammation revealed fetuin-A-dependent upregulation of TBX21 (a Th1 cell-specific transcription factor) in the lungs of Cm-infected WT mice that correlated with IFN-γ induction. Additionally, the effect of fetuin-A deficiency in mounting an adaptive immune response to Cm infection was demonstrated using a splenocyte recall assay. Although preliminary in nature, these findings are suggestive of fetuin-A involvement following Cm pulmonary infection and underscores the need to investigate further the role of fetuin-A in the immune response and the consequences of its gene deletion. |
format | Online Article Text |
id | pubmed-9042633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-90426332022-04-27 Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection Mahjabeen, Faria Yu, Jieh-Juen Chambers, James P. Gupta, Rishein Arulanandam, Bernard P. Int J Microbiol Research Article Fetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased 46%, while INF-γ increased 44%, consistent with a negative regulatory role of fetuin-A in inflammation. Importantly, the observed increased IFN-γ production was abrogated in fetuin-A-deficient AHSG mice suggesting that IFN-γ induction following Cm infection is fetuin-A dependent. Assessment of expression of genes associated with inflammation revealed fetuin-A-dependent upregulation of TBX21 (a Th1 cell-specific transcription factor) in the lungs of Cm-infected WT mice that correlated with IFN-γ induction. Additionally, the effect of fetuin-A deficiency in mounting an adaptive immune response to Cm infection was demonstrated using a splenocyte recall assay. Although preliminary in nature, these findings are suggestive of fetuin-A involvement following Cm pulmonary infection and underscores the need to investigate further the role of fetuin-A in the immune response and the consequences of its gene deletion. Hindawi 2022-04-19 /pmc/articles/PMC9042633/ /pubmed/35498397 http://dx.doi.org/10.1155/2022/6082140 Text en Copyright © 2022 Faria Mahjabeen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Mahjabeen, Faria Yu, Jieh-Juen Chambers, James P. Gupta, Rishein Arulanandam, Bernard P. Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
title | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
title_full | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
title_fullStr | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
title_full_unstemmed | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
title_short | Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection |
title_sort | influence of fetuin-a on chlamydia muridarum pulmonary infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042633/ https://www.ncbi.nlm.nih.gov/pubmed/35498397 http://dx.doi.org/10.1155/2022/6082140 |
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