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Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection

Fetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased...

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Autores principales: Mahjabeen, Faria, Yu, Jieh-Juen, Chambers, James P., Gupta, Rishein, Arulanandam, Bernard P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042633/
https://www.ncbi.nlm.nih.gov/pubmed/35498397
http://dx.doi.org/10.1155/2022/6082140
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author Mahjabeen, Faria
Yu, Jieh-Juen
Chambers, James P.
Gupta, Rishein
Arulanandam, Bernard P.
author_facet Mahjabeen, Faria
Yu, Jieh-Juen
Chambers, James P.
Gupta, Rishein
Arulanandam, Bernard P.
author_sort Mahjabeen, Faria
collection PubMed
description Fetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased 46%, while INF-γ increased 44%, consistent with a negative regulatory role of fetuin-A in inflammation. Importantly, the observed increased IFN-γ production was abrogated in fetuin-A-deficient AHSG mice suggesting that IFN-γ induction following Cm infection is fetuin-A dependent. Assessment of expression of genes associated with inflammation revealed fetuin-A-dependent upregulation of TBX21 (a Th1 cell-specific transcription factor) in the lungs of Cm-infected WT mice that correlated with IFN-γ induction. Additionally, the effect of fetuin-A deficiency in mounting an adaptive immune response to Cm infection was demonstrated using a splenocyte recall assay. Although preliminary in nature, these findings are suggestive of fetuin-A involvement following Cm pulmonary infection and underscores the need to investigate further the role of fetuin-A in the immune response and the consequences of its gene deletion.
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spelling pubmed-90426332022-04-27 Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection Mahjabeen, Faria Yu, Jieh-Juen Chambers, James P. Gupta, Rishein Arulanandam, Bernard P. Int J Microbiol Research Article Fetuin-A is an acute phase glycoprotein shown to counter in a regulatory manner proinflammatory cytokine production to maintain homeostasis during inflammation. We report here that in wild-type mice 12 days after Chlamydia muridarum (Cm) intranasal challenge, fetuin-A content in the lungs decreased 46%, while INF-γ increased 44%, consistent with a negative regulatory role of fetuin-A in inflammation. Importantly, the observed increased IFN-γ production was abrogated in fetuin-A-deficient AHSG mice suggesting that IFN-γ induction following Cm infection is fetuin-A dependent. Assessment of expression of genes associated with inflammation revealed fetuin-A-dependent upregulation of TBX21 (a Th1 cell-specific transcription factor) in the lungs of Cm-infected WT mice that correlated with IFN-γ induction. Additionally, the effect of fetuin-A deficiency in mounting an adaptive immune response to Cm infection was demonstrated using a splenocyte recall assay. Although preliminary in nature, these findings are suggestive of fetuin-A involvement following Cm pulmonary infection and underscores the need to investigate further the role of fetuin-A in the immune response and the consequences of its gene deletion. Hindawi 2022-04-19 /pmc/articles/PMC9042633/ /pubmed/35498397 http://dx.doi.org/10.1155/2022/6082140 Text en Copyright © 2022 Faria Mahjabeen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Mahjabeen, Faria
Yu, Jieh-Juen
Chambers, James P.
Gupta, Rishein
Arulanandam, Bernard P.
Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection
title Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection
title_full Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection
title_fullStr Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection
title_full_unstemmed Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection
title_short Influence of Fetuin-A on Chlamydia muridarum Pulmonary Infection
title_sort influence of fetuin-a on chlamydia muridarum pulmonary infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042633/
https://www.ncbi.nlm.nih.gov/pubmed/35498397
http://dx.doi.org/10.1155/2022/6082140
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