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Ratiometric fluorescence assay based on carbon dots and Cu(2+)-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox

The monitoring of deferasirox (DEF) has important clinical roles in patients who need iron excretion. However, analytical methods with practicability and simplicity are limited. Moreover, ratiometric fluorescence strategies based on Förster resonance energy transfer (FRET) from carbon dots (CDs) as...

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Autores principales: Miao, Chen-Fang, Guo, Xian-Zhong, Zhang, Xin-Tian, Lin, Yin-Ning, Han, Wen-Di, Huang, Zheng-Jun, Weng, Shao-Huang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042915/
https://www.ncbi.nlm.nih.gov/pubmed/35494749
http://dx.doi.org/10.1039/d1ra07078a
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author Miao, Chen-Fang
Guo, Xian-Zhong
Zhang, Xin-Tian
Lin, Yin-Ning
Han, Wen-Di
Huang, Zheng-Jun
Weng, Shao-Huang
author_facet Miao, Chen-Fang
Guo, Xian-Zhong
Zhang, Xin-Tian
Lin, Yin-Ning
Han, Wen-Di
Huang, Zheng-Jun
Weng, Shao-Huang
author_sort Miao, Chen-Fang
collection PubMed
description The monitoring of deferasirox (DEF) has important clinical roles in patients who need iron excretion. However, analytical methods with practicability and simplicity are limited. Moreover, ratiometric fluorescence strategies based on Förster resonance energy transfer (FRET) from carbon dots (CDs) as a donor are rarely reported as a drug monitor. In this work, CDs with an appropriate emitting wavelength at 480 nm and excitation around 370 nm were prepared by hydrothermal approach and HCl post-treatment. O-Phenylenediamine (OPD) can be oxidized by Cu(2+) to produce yellow fluorescent 2,3-diaminophenazine (oxOPD) in the system of Cu(2+) and OPD (Cu–OPD). Correspondingly, a remarkable FRET from CDs to oxOPD in the system of CDs, Cu(2+) and OPD (CDs–Cu–OPD) was fabricated with the quenching illustration of CDs, but emitting property of oxOPD. Attributed to the chelation ability of DEF on Cu(2+), the inhibitory effects of DEF on the Cu(2+)-triggered oxidative capability reduced the FRET system by the decreased oxOPD. Thus, the recovered CDs at F(480) and decreased oxOPD at F(560) were found through a ratiometric mode by the addition of DEF in CDs–Cu–OPD for the DEF assay. The FRET behavior of CDs and oxOPD in CDs–Cu–OPD was proved clearly through the calculation of the association constant, binding constant, number of binding sites, and the distance between the donor and acceptor. Furthermore, this ratiometric method exhibited promising analytical performance for DEF with the application in real samples. The implementation of this work expands the application field of CDs and OPD oxidation in drug monitoring, and even other biological analyses through ratiometric strategy.
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spelling pubmed-90429152022-04-28 Ratiometric fluorescence assay based on carbon dots and Cu(2+)-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox Miao, Chen-Fang Guo, Xian-Zhong Zhang, Xin-Tian Lin, Yin-Ning Han, Wen-Di Huang, Zheng-Jun Weng, Shao-Huang RSC Adv Chemistry The monitoring of deferasirox (DEF) has important clinical roles in patients who need iron excretion. However, analytical methods with practicability and simplicity are limited. Moreover, ratiometric fluorescence strategies based on Förster resonance energy transfer (FRET) from carbon dots (CDs) as a donor are rarely reported as a drug monitor. In this work, CDs with an appropriate emitting wavelength at 480 nm and excitation around 370 nm were prepared by hydrothermal approach and HCl post-treatment. O-Phenylenediamine (OPD) can be oxidized by Cu(2+) to produce yellow fluorescent 2,3-diaminophenazine (oxOPD) in the system of Cu(2+) and OPD (Cu–OPD). Correspondingly, a remarkable FRET from CDs to oxOPD in the system of CDs, Cu(2+) and OPD (CDs–Cu–OPD) was fabricated with the quenching illustration of CDs, but emitting property of oxOPD. Attributed to the chelation ability of DEF on Cu(2+), the inhibitory effects of DEF on the Cu(2+)-triggered oxidative capability reduced the FRET system by the decreased oxOPD. Thus, the recovered CDs at F(480) and decreased oxOPD at F(560) were found through a ratiometric mode by the addition of DEF in CDs–Cu–OPD for the DEF assay. The FRET behavior of CDs and oxOPD in CDs–Cu–OPD was proved clearly through the calculation of the association constant, binding constant, number of binding sites, and the distance between the donor and acceptor. Furthermore, this ratiometric method exhibited promising analytical performance for DEF with the application in real samples. The implementation of this work expands the application field of CDs and OPD oxidation in drug monitoring, and even other biological analyses through ratiometric strategy. The Royal Society of Chemistry 2021-10-25 /pmc/articles/PMC9042915/ /pubmed/35494749 http://dx.doi.org/10.1039/d1ra07078a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Miao, Chen-Fang
Guo, Xian-Zhong
Zhang, Xin-Tian
Lin, Yin-Ning
Han, Wen-Di
Huang, Zheng-Jun
Weng, Shao-Huang
Ratiometric fluorescence assay based on carbon dots and Cu(2+)-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox
title Ratiometric fluorescence assay based on carbon dots and Cu(2+)-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox
title_full Ratiometric fluorescence assay based on carbon dots and Cu(2+)-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox
title_fullStr Ratiometric fluorescence assay based on carbon dots and Cu(2+)-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox
title_full_unstemmed Ratiometric fluorescence assay based on carbon dots and Cu(2+)-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox
title_short Ratiometric fluorescence assay based on carbon dots and Cu(2+)-catalyzed oxidation of O-phenylenediamine for the effective detection of deferasirox
title_sort ratiometric fluorescence assay based on carbon dots and cu(2+)-catalyzed oxidation of o-phenylenediamine for the effective detection of deferasirox
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9042915/
https://www.ncbi.nlm.nih.gov/pubmed/35494749
http://dx.doi.org/10.1039/d1ra07078a
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