Cargando…

Methods for the Cost-Effective Production of Bacteria-Derived Double-Stranded RNA for in vitro Knockdown Studies

RNA interference (RNAi) is a highly conserved pathway for the post-transcriptional regulation of gene expression. It has become a crucial tool in life science research, with promising potential for pest-management applications. To induce an RNAi response, long double-stranded RNA (dsRNA) sequences s...

Descripción completa

Detalles Bibliográficos
Autores principales:	Verdonckt, Thomas-Wolf, Vanden Broeck, Jozef
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Physiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043282/ https://www.ncbi.nlm.nih.gov/pubmed/35492592 http://dx.doi.org/10.3389/fphys.2022.836106

Ejemplares similares

RNA Interference in Insects: Protecting Beneficials and Controlling Pests
por: Vogel, Elise, et al.
Publicado: (2019)

RNAi-Mediated Knockdown of Transcription Factor E93 in Nymphs of the Desert Locust (Schistocerca gregaria) Inhibits Adult Morphogenesis and Results in Supernumerary Juvenile Stages
por: Gijbels, Marijke, et al.
Publicado: (2020)

The possible impact of persistent virus infection on the function of the RNAi machinery in insects: a hypothesis
por: Swevers, Luc, et al.
Publicado: (2013)

Eat to reproduce: a key role for the insulin signaling pathway in adult insects
por: Badisco, Liesbeth, et al.
Publicado: (2013)

Identification and Profiling of a Novel Bombyx mori latent virus Variant Acutely Infecting Helicoverpa armigera and Trichoplusia ni
por: Verdonckt, Thomas-Wolf, et al.
Publicado: (2023)

Double Knockdown of PHD1 and Keap1 Attenuated Hypoxia-Induced Injuries in Hepatocytes
por: Liu, Jing, et al.
Publicado: (2017)

Insights into RNAi-based antiviral immunity in Lepidoptera: acute and persistent infections in Bombyx mori and Trichoplusia ni cell lines
por: Santos, Dulce, et al.
Publicado: (2018)

Cloning and Functional Characterization of a Double-Stranded RNA-Degrading Nuclease in the Tawny Crazy Ant (Nylanderia fulva)
por: Lei, Jiaxin, et al.
Publicado: (2022)

Post-feeding transcriptomics reveals essential genes expressed in the midgut of the desert locust
por: Van Lommel, Joachim, et al.
Publicado: (2023)

PIWI Proteins Play an Antiviral Role in Lepidopteran Cell Lines
por: Santos, Dulce, et al.
Publicado: (2022)

RNA Interference Is Enhanced by Knockdown of Double-Stranded RNases in the Yellow Fever Mosquito Aedes aegypti
por: Giesbrecht, David, et al.
Publicado: (2020)

XIAP Knockdown in Alcohol-Associated Liver Disease Models Exhibits Divergent in vitro and in vivo Phenotypes Owing to a Potential Zonal Inhibitory Role of SMAC
por: He, Li, et al.
Publicado: (2021)

Knockdown of the Halloween Genes spook, shadow and shade Influences Oocyte Development, Egg Shape, Oviposition and Hatching in the Desert Locust
por: Schellens, Sam, et al.
Publicado: (2022)

Production of Double-stranded DNA Ministrings
por: Wong, Shirley, et al.
Publicado: (2016)

mRNA knockdown by single strand RNA is improved by chemical modifications
por: Haringsma, Henry J., et al.
Publicado: (2012)

Diminished Exercise Capacity and Mitochondrial bc1 Complex Deficiency in Tafazzin-Knockdown Mice
por: Powers, Corey, et al.
Publicado: (2013)

Glycoprotein Hormone Receptor Knockdown Leads to Reduced Reproductive Success in Male Aedes aegypti
por: Rocco, David A., et al.
Publicado: (2019)

β-Catenin Knockdown Affects Mitochondrial Biogenesis and Lipid Metabolism in Breast Cancer Cells
por: Vergara, Daniele, et al.
Publicado: (2017)

Long Non-coding RNA MIAT Knockdown Prevents the Formation of Intracranial Aneurysm by Downregulating ENC1 via MYC
por: Li, Xinguo, et al.
Publicado: (2021)

Knockdown of SLC38 Transporter Ortholog – CG13743 Reveals a Metabolic Relevance in Drosophila
por: Aggarwal, Tanya, et al.
Publicado: (2020)

Microbial-Based Double-Stranded RNA Production to Develop Cost-Effective RNA Interference Application for Insect Pest Management
por: Ahn, Seung-Joon, et al.
Publicado: (2019)

Analysis of Guanine Oxidation Products in Double-Stranded DNA and Proposed Guanine Oxidation Pathways in Single-Stranded, Double-Stranded or Quadruplex DNA
por: Morikawa, Masayuki, et al.
Publicado: (2014)

Knockdown of CNN3 Impairs Myoblast Proliferation, Differentiation, and Protein Synthesis via the mTOR Pathway
por: She, Yanling, et al.
Publicado: (2021)

Knockdown of Ift88 in fibroblasts causes extracellular matrix remodeling and decreases conduction velocity in cardiomyocyte monolayers
por: Ernault, Auriane C., et al.
Publicado: (2022)

Knockdown of Death-Associated Protein Expression Induces Global Transcriptome Changes in Proliferating and Differentiating Muscle Satellite Cells
por: Horton, Katherine A., et al.
Publicado: (2020)

Transcriptome Analysis and Knockdown of the Juvenile Hormone Esterase Gene Reveal Abnormal Feeding Behavior in the Sugarcane Giant Borer
por: Noriega, Daniel D., et al.
Publicado: (2020)

Circumventricular Organ Apelin Receptor Knockdown Decreases Blood Pressure and Sympathetic Drive Responses in the Spontaneously Hypertensive Rat
por: Griffiths, Philip R., et al.
Publicado: (2021)

Decompression vs. Decomposition: Distribution, Amount, and Gas Composition of Bubbles in Stranded Marine Mammals
por: de Quirós, Yara Bernaldo, et al.
Publicado: (2012)

Piezo2 Knockdown Inhibits Noxious Mechanical Stimulation and NGF-Induced Sensitization in A-Delta Bone Afferent Neurons
por: Nencini, Sara, et al.
Publicado: (2021)

Chloride intracellular channel gene knockdown induces insect cell lines death and level increases of intracellular calcium ions
por: Song, Jiqiang, et al.
Publicado: (2023)

Endogenous Double-Stranded RNA
por: Sadeq, Shaymaa, et al.
Publicado: (2021)

In vitro Assays and Imaging Methods for Drug Discovery for Cardiac Fibrosis
por: Palano, Giorgia, et al.
Publicado: (2021)

Improvement of Vascular Function by Knockdown of Salusin-β in Hypertensive Rats via Nitric Oxide and Reactive Oxygen Species Signaling Pathway
por: Pan, Yan, et al.
Publicado: (2021)

Knockdown of a Cyclic Nucleotide-Gated Ion Channel Impairs Locomotor Activity and Recovery From Hypoxia in Adult Drosophila melanogaster
por: Qiu, Shuang, et al.
Publicado: (2022)

Study on the Effect of Wing Bud Chitin Metabolism and Its Developmental Network Genes in the Brown Planthopper, Nilaparvata lugens, by Knockdown of TRE Gene
por: Zhang, Lu, et al.
Publicado: (2017)

Knockdown of carnitine palmitoyltransferase I (CPT1) reduces fat body lipid mobilization and resistance to starvation in the insect vector Rhodnius prolixus
por: De Paula, Iron F., et al.
Publicado: (2023)

Rapid in vitro production of single-stranded DNA
por: Minev, Dionis, et al.
Publicado: (2019)

Double-stranded RNA reduction by chaotropic agents during in vitro transcription of messenger RNA
por: Piao, Xijun, et al.
Publicado: (2022)

Tumor necrosis factor alpha and palmitate simulate bovine fatty liver disease in vitro when using abattoir-derived primary bovine hepatocytes isolated by a novel nonperfusion method
por: Testroet, E.D., et al.
Publicado: (2022)

Knockdown of APOPT1/COA8 Causes Cytochrome c Oxidase Deficiency, Neuromuscular Impairment, and Reduced Resistance to Oxidative Stress in Drosophila melanogaster
por: Brischigliaro, Michele, et al.
Publicado: (2019)

Cannot write session to /tmp/vufind_sessions/sess_jqehnm6bl5lslufokb13k6iovm