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Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X
Thermosensitive genic male sterility (TGMS) has been widely used in two-line hybrid rice breeding. Due to hybrid seed production being highly affected by changeable environments, its application scope is limited to some extent. Thus, it is of great importance to identify potential TGMS genes in spec...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kunming Institute of Botany, Chinese Academy of Sciences
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043303/ https://www.ncbi.nlm.nih.gov/pubmed/35505986 http://dx.doi.org/10.1016/j.pld.2021.05.002 |
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author | Zhang, Xiao Chang, Guimei Wu, Zihao Wan, Jinpeng Yang, Jun Wang, Feijun Wang, Fang Yu, Diqiu Xu, Peng |
author_facet | Zhang, Xiao Chang, Guimei Wu, Zihao Wan, Jinpeng Yang, Jun Wang, Feijun Wang, Fang Yu, Diqiu Xu, Peng |
author_sort | Zhang, Xiao |
collection | PubMed |
description | Thermosensitive genic male sterility (TGMS) has been widely used in two-line hybrid rice breeding. Due to hybrid seed production being highly affected by changeable environments, its application scope is limited to some extent. Thus, it is of great importance to identify potential TGMS genes in specific rice varieties. Here, Diannong S-1 xuan (DNS-1X), a reverse TGMS (RTGMS) japonica male sterile line, was identified from Diannong S-1. Genetic analysis showed that male sterility was tightly controlled by a single recessive gene, which was supported by the phenotype of the F(1) and F(2:3) populations derived from the cross between DNS-1X and Yunjing 26 (YJ26). Combining simple sequence repeat (SSR) markers and bulked segregation analysis (BSA), we identified a 215 kb region on chromosome 10 as a candidate reverse TGMS region, which was designated as rtms1-D. It was narrower than the previously reported RTGMS genes rtms1 and tms6(t). The fertility conversion detected in the natural environment showed that DNS-1X was sterile below 28–30 °C; otherwise, it was fertile. Histological analysis further indicated that the pollen abortion was occurred in the young microspore stage. This study will provide new resources for two-line hybrid rice and pave the way for molecular breeding of RTGMS lines. |
format | Online Article Text |
id | pubmed-9043303 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Kunming Institute of Botany, Chinese Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-90433032022-05-02 Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X Zhang, Xiao Chang, Guimei Wu, Zihao Wan, Jinpeng Yang, Jun Wang, Feijun Wang, Fang Yu, Diqiu Xu, Peng Plant Divers Research Paper Thermosensitive genic male sterility (TGMS) has been widely used in two-line hybrid rice breeding. Due to hybrid seed production being highly affected by changeable environments, its application scope is limited to some extent. Thus, it is of great importance to identify potential TGMS genes in specific rice varieties. Here, Diannong S-1 xuan (DNS-1X), a reverse TGMS (RTGMS) japonica male sterile line, was identified from Diannong S-1. Genetic analysis showed that male sterility was tightly controlled by a single recessive gene, which was supported by the phenotype of the F(1) and F(2:3) populations derived from the cross between DNS-1X and Yunjing 26 (YJ26). Combining simple sequence repeat (SSR) markers and bulked segregation analysis (BSA), we identified a 215 kb region on chromosome 10 as a candidate reverse TGMS region, which was designated as rtms1-D. It was narrower than the previously reported RTGMS genes rtms1 and tms6(t). The fertility conversion detected in the natural environment showed that DNS-1X was sterile below 28–30 °C; otherwise, it was fertile. Histological analysis further indicated that the pollen abortion was occurred in the young microspore stage. This study will provide new resources for two-line hybrid rice and pave the way for molecular breeding of RTGMS lines. Kunming Institute of Botany, Chinese Academy of Sciences 2021-06-01 /pmc/articles/PMC9043303/ /pubmed/35505986 http://dx.doi.org/10.1016/j.pld.2021.05.002 Text en © 2021 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper Zhang, Xiao Chang, Guimei Wu, Zihao Wan, Jinpeng Yang, Jun Wang, Feijun Wang, Fang Yu, Diqiu Xu, Peng Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X |
title | Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X |
title_full | Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X |
title_fullStr | Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X |
title_full_unstemmed | Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X |
title_short | Identification and fine mapping of rtms1-D, a gene responsible for reverse thermosensitive genic male sterility from Diannong S-1X |
title_sort | identification and fine mapping of rtms1-d, a gene responsible for reverse thermosensitive genic male sterility from diannong s-1x |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043303/ https://www.ncbi.nlm.nih.gov/pubmed/35505986 http://dx.doi.org/10.1016/j.pld.2021.05.002 |
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