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A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide
Efficient intracellular drug delivery in nanomedicine strongly depends on ways to induce cellular uptake. Conjugation of nanoparticles (NPs) with cell-penetrating peptides (CPPs) is a known means to induce uptake via endocytosis. Here, we functionalized NPs consisting of either poly(d,l-lactide-co-g...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043423/ https://www.ncbi.nlm.nih.gov/pubmed/35492790 http://dx.doi.org/10.1039/d1ra05871a |
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author | Paulino da Silva Filho, Omar Ali, Muhanad Nabbefeld, Rike Primavessy, Daniel Bovee-Geurts, Petra H. Grimm, Silko Kirchner, Andreas Wiesmüller, Karl-Heinz Schneider, Marc Walboomers, X. Frank Brock, Roland |
author_facet | Paulino da Silva Filho, Omar Ali, Muhanad Nabbefeld, Rike Primavessy, Daniel Bovee-Geurts, Petra H. Grimm, Silko Kirchner, Andreas Wiesmüller, Karl-Heinz Schneider, Marc Walboomers, X. Frank Brock, Roland |
author_sort | Paulino da Silva Filho, Omar |
collection | PubMed |
description | Efficient intracellular drug delivery in nanomedicine strongly depends on ways to induce cellular uptake. Conjugation of nanoparticles (NPs) with cell-penetrating peptides (CPPs) is a known means to induce uptake via endocytosis. Here, we functionalized NPs consisting of either poly(d,l-lactide-co-glycolide) (PLGA) or polyethene glycol (PEG)-PLGA block-copolymer with a lactoferrin-derived cell-penetrating peptide (hLF). To enhance the association between the peptide and the polymer NPs, we tested a range of acyl moieties for N-terminal acylation of the peptide as a means to promote noncovalent interactions. Acyl moieties differed in chain length and number of acyl chains. Peptide-functionalized NPs were characterized for nanoparticle size, overall net charge, storage stability, and intracellular uptake. Coating particles with a palmitoylated hLF resulted in minimal precipitation after storage at −20C and homogeneous particle size (<200 nm). Palmitoyl-hLF coated NPs showed enhanced delivery in different cells in comparison to NPs lacking functionalization. Moreover, in comparison to acetyl-hLF, palmitoyl-hLF was also suited for coating and enhancing the cellular uptake of PEG-PLGA NPs. |
format | Online Article Text |
id | pubmed-9043423 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90434232022-04-28 A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide Paulino da Silva Filho, Omar Ali, Muhanad Nabbefeld, Rike Primavessy, Daniel Bovee-Geurts, Petra H. Grimm, Silko Kirchner, Andreas Wiesmüller, Karl-Heinz Schneider, Marc Walboomers, X. Frank Brock, Roland RSC Adv Chemistry Efficient intracellular drug delivery in nanomedicine strongly depends on ways to induce cellular uptake. Conjugation of nanoparticles (NPs) with cell-penetrating peptides (CPPs) is a known means to induce uptake via endocytosis. Here, we functionalized NPs consisting of either poly(d,l-lactide-co-glycolide) (PLGA) or polyethene glycol (PEG)-PLGA block-copolymer with a lactoferrin-derived cell-penetrating peptide (hLF). To enhance the association between the peptide and the polymer NPs, we tested a range of acyl moieties for N-terminal acylation of the peptide as a means to promote noncovalent interactions. Acyl moieties differed in chain length and number of acyl chains. Peptide-functionalized NPs were characterized for nanoparticle size, overall net charge, storage stability, and intracellular uptake. Coating particles with a palmitoylated hLF resulted in minimal precipitation after storage at −20C and homogeneous particle size (<200 nm). Palmitoyl-hLF coated NPs showed enhanced delivery in different cells in comparison to NPs lacking functionalization. Moreover, in comparison to acetyl-hLF, palmitoyl-hLF was also suited for coating and enhancing the cellular uptake of PEG-PLGA NPs. The Royal Society of Chemistry 2021-11-10 /pmc/articles/PMC9043423/ /pubmed/35492790 http://dx.doi.org/10.1039/d1ra05871a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Paulino da Silva Filho, Omar Ali, Muhanad Nabbefeld, Rike Primavessy, Daniel Bovee-Geurts, Petra H. Grimm, Silko Kirchner, Andreas Wiesmüller, Karl-Heinz Schneider, Marc Walboomers, X. Frank Brock, Roland A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide |
title | A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide |
title_full | A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide |
title_fullStr | A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide |
title_full_unstemmed | A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide |
title_short | A comparison of acyl-moieties for noncovalent functionalization of PLGA and PEG-PLGA nanoparticles with a cell-penetrating peptide |
title_sort | comparison of acyl-moieties for noncovalent functionalization of plga and peg-plga nanoparticles with a cell-penetrating peptide |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043423/ https://www.ncbi.nlm.nih.gov/pubmed/35492790 http://dx.doi.org/10.1039/d1ra05871a |
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