Cargando…

Mitoapocynin Attenuates Organic Dust Exposure-Induced Neuroinflammation and Sensory-Motor Deficits in a Mouse Model

Increased incidences of neuro-inflammatory diseases in the mid-western United States of America (USA) have been linked to exposure to agriculture contaminants. Organic dust (OD) is a major contaminant in the animal production industry and is central to the respiratory symptoms in the exposed individ...

Descripción completa

Detalles Bibliográficos
Autores principales: Massey, Nyzil, Shrestha, Denusha, Bhat, Sanjana Mahadev, Padhi, Piyush, Wang, Chong, Karriker, Locke A., Smith, Jodi D., Kanthasamy, Anumantha G., Charavaryamath, Chandrashekhar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043522/
https://www.ncbi.nlm.nih.gov/pubmed/35496912
http://dx.doi.org/10.3389/fncel.2022.817046
_version_ 1784694899723993088
author Massey, Nyzil
Shrestha, Denusha
Bhat, Sanjana Mahadev
Padhi, Piyush
Wang, Chong
Karriker, Locke A.
Smith, Jodi D.
Kanthasamy, Anumantha G.
Charavaryamath, Chandrashekhar
author_facet Massey, Nyzil
Shrestha, Denusha
Bhat, Sanjana Mahadev
Padhi, Piyush
Wang, Chong
Karriker, Locke A.
Smith, Jodi D.
Kanthasamy, Anumantha G.
Charavaryamath, Chandrashekhar
author_sort Massey, Nyzil
collection PubMed
description Increased incidences of neuro-inflammatory diseases in the mid-western United States of America (USA) have been linked to exposure to agriculture contaminants. Organic dust (OD) is a major contaminant in the animal production industry and is central to the respiratory symptoms in the exposed individuals. However, the exposure effects on the brain remain largely unknown. OD exposure is known to induce a pro-inflammatory phenotype in microglial cells. Further, blocking cytoplasmic NOX-2 using mitoapocynin (MA) partially curtail the OD exposure effects. Therefore, using a mouse model, we tested a hypothesis that inhaled OD induces neuroinflammation and sensory-motor deficits. Mice were administered with either saline, fluorescent lipopolysaccharides (LPSs), or OD extract intranasally daily for 5 days a week for 5 weeks. The saline or OD extract-exposed mice received either a vehicle or MA (3 mg/kg) orally for 3 days/week for 5 weeks. We quantified inflammatory changes in the upper respiratory tract and brain, assessed sensory-motor changes using rotarod, open-field, and olfactory test, and quantified neurochemicals in the brain. Inhaled fluorescent LPS (FL-LPS) was detected in the nasal turbinates and olfactory bulbs. OD extract exposure induced atrophy of the olfactory epithelium with reduction in the number of nerve bundles in the nasopharyngeal meatus, loss of cilia in the upper respiratory epithelium with an increase in the number of goblet cells, and increase in the thickness of the nasal epithelium. Interestingly, OD exposure increased the expression of HMGB1, 3- nitrotyrosine (NT), IBA1, glial fibrillary acidic protein (GFAP), hyperphosphorylated Tau (p-Tau), and terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL)-positive cells in the brain. Further, OD exposure decreased time to fall (rotarod), total distance traveled (open-field test), and olfactory ability (novel scent test). Oral MA partially rescued olfactory epithelial changes and gross congestion of the brain tissue. MA treatment also decreased the expression of HMGB1, 3-NT, IBA1, GFAP, and p-Tau, and significantly reversed exposure induced sensory-motor deficits. Neurochemical analysis provided an early indication of depressive behavior. Collectively, our results demonstrate that inhalation exposure to OD can cause sustained neuroinflammation and behavior deficits through lung-brain axis and that MA treatment can dampen the OD-induced inflammatory response at the level of lung and brain.
format Online
Article
Text
id pubmed-9043522
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-90435222022-04-28 Mitoapocynin Attenuates Organic Dust Exposure-Induced Neuroinflammation and Sensory-Motor Deficits in a Mouse Model Massey, Nyzil Shrestha, Denusha Bhat, Sanjana Mahadev Padhi, Piyush Wang, Chong Karriker, Locke A. Smith, Jodi D. Kanthasamy, Anumantha G. Charavaryamath, Chandrashekhar Front Cell Neurosci Cellular Neuroscience Increased incidences of neuro-inflammatory diseases in the mid-western United States of America (USA) have been linked to exposure to agriculture contaminants. Organic dust (OD) is a major contaminant in the animal production industry and is central to the respiratory symptoms in the exposed individuals. However, the exposure effects on the brain remain largely unknown. OD exposure is known to induce a pro-inflammatory phenotype in microglial cells. Further, blocking cytoplasmic NOX-2 using mitoapocynin (MA) partially curtail the OD exposure effects. Therefore, using a mouse model, we tested a hypothesis that inhaled OD induces neuroinflammation and sensory-motor deficits. Mice were administered with either saline, fluorescent lipopolysaccharides (LPSs), or OD extract intranasally daily for 5 days a week for 5 weeks. The saline or OD extract-exposed mice received either a vehicle or MA (3 mg/kg) orally for 3 days/week for 5 weeks. We quantified inflammatory changes in the upper respiratory tract and brain, assessed sensory-motor changes using rotarod, open-field, and olfactory test, and quantified neurochemicals in the brain. Inhaled fluorescent LPS (FL-LPS) was detected in the nasal turbinates and olfactory bulbs. OD extract exposure induced atrophy of the olfactory epithelium with reduction in the number of nerve bundles in the nasopharyngeal meatus, loss of cilia in the upper respiratory epithelium with an increase in the number of goblet cells, and increase in the thickness of the nasal epithelium. Interestingly, OD exposure increased the expression of HMGB1, 3- nitrotyrosine (NT), IBA1, glial fibrillary acidic protein (GFAP), hyperphosphorylated Tau (p-Tau), and terminal deoxynucleotidyl transferase deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL)-positive cells in the brain. Further, OD exposure decreased time to fall (rotarod), total distance traveled (open-field test), and olfactory ability (novel scent test). Oral MA partially rescued olfactory epithelial changes and gross congestion of the brain tissue. MA treatment also decreased the expression of HMGB1, 3-NT, IBA1, GFAP, and p-Tau, and significantly reversed exposure induced sensory-motor deficits. Neurochemical analysis provided an early indication of depressive behavior. Collectively, our results demonstrate that inhalation exposure to OD can cause sustained neuroinflammation and behavior deficits through lung-brain axis and that MA treatment can dampen the OD-induced inflammatory response at the level of lung and brain. Frontiers Media S.A. 2022-04-13 /pmc/articles/PMC9043522/ /pubmed/35496912 http://dx.doi.org/10.3389/fncel.2022.817046 Text en Copyright © 2022 Massey, Shrestha, Bhat, Padhi, Wang, Karriker, Smith, Kanthasamy and Charavaryamath. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Massey, Nyzil
Shrestha, Denusha
Bhat, Sanjana Mahadev
Padhi, Piyush
Wang, Chong
Karriker, Locke A.
Smith, Jodi D.
Kanthasamy, Anumantha G.
Charavaryamath, Chandrashekhar
Mitoapocynin Attenuates Organic Dust Exposure-Induced Neuroinflammation and Sensory-Motor Deficits in a Mouse Model
title Mitoapocynin Attenuates Organic Dust Exposure-Induced Neuroinflammation and Sensory-Motor Deficits in a Mouse Model
title_full Mitoapocynin Attenuates Organic Dust Exposure-Induced Neuroinflammation and Sensory-Motor Deficits in a Mouse Model
title_fullStr Mitoapocynin Attenuates Organic Dust Exposure-Induced Neuroinflammation and Sensory-Motor Deficits in a Mouse Model
title_full_unstemmed Mitoapocynin Attenuates Organic Dust Exposure-Induced Neuroinflammation and Sensory-Motor Deficits in a Mouse Model
title_short Mitoapocynin Attenuates Organic Dust Exposure-Induced Neuroinflammation and Sensory-Motor Deficits in a Mouse Model
title_sort mitoapocynin attenuates organic dust exposure-induced neuroinflammation and sensory-motor deficits in a mouse model
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043522/
https://www.ncbi.nlm.nih.gov/pubmed/35496912
http://dx.doi.org/10.3389/fncel.2022.817046
work_keys_str_mv AT masseynyzil mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel
AT shresthadenusha mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel
AT bhatsanjanamahadev mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel
AT padhipiyush mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel
AT wangchong mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel
AT karrikerlockea mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel
AT smithjodid mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel
AT kanthasamyanumanthag mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel
AT charavaryamathchandrashekhar mitoapocyninattenuatesorganicdustexposureinducedneuroinflammationandsensorymotordeficitsinamousemodel