Imaging of the skin microvascularization using spatially depolarized dynamic speckle

SIGNIFICANCE: We propose a technique devoted to real-time high-resolution imaging of skin microvascularization. AIM: The process utilizes the temporal variation of the spatially depolarized optical speckle field generated by moving red blood cells when illuminated with fully polarized coherent light. APPROACH: Polarimetric filtering prevents the contribution of surface scattering from reaching the camera and thus favors the detection of multiscattered photons from the deeper layers of the skin. RESULTS: Full-field images reveal the microvasculature with a spatial resolution of [Formula: see text]. The acquisition speed allows for real-time applications. CONCLUSIONS: We demonstrate the ability of this method to determine in 1 s a stable and reliable microvascular activity, enabling numerous clinical applications that require quantitative measurements.