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Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes

African swine fever (ASF) is a highly contact infectious disease caused by the African swine fever virus (ASFV). The extremely complex structure and infection mechanism make it difficult to control the spread of ASFV and develop the vaccine. The ASFV genome is huge with many antigenic genes. Among t...

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Autores principales: Chen, Liyi, Zhang, Xinheng, Shao, Guanming, Shao, Yangyang, Hu, Zezhong, Feng, Keyu, Xie, Zi, Li, Hongxin, Chen, Weiguo, Lin, Wencheng, Yuan, Hengxing, Wang, Hailong, Fu, Jun, Xie, Qingmei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043850/
https://www.ncbi.nlm.nih.gov/pubmed/35498728
http://dx.doi.org/10.3389/fvets.2022.832255
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author Chen, Liyi
Zhang, Xinheng
Shao, Guanming
Shao, Yangyang
Hu, Zezhong
Feng, Keyu
Xie, Zi
Li, Hongxin
Chen, Weiguo
Lin, Wencheng
Yuan, Hengxing
Wang, Hailong
Fu, Jun
Xie, Qingmei
author_facet Chen, Liyi
Zhang, Xinheng
Shao, Guanming
Shao, Yangyang
Hu, Zezhong
Feng, Keyu
Xie, Zi
Li, Hongxin
Chen, Weiguo
Lin, Wencheng
Yuan, Hengxing
Wang, Hailong
Fu, Jun
Xie, Qingmei
author_sort Chen, Liyi
collection PubMed
description African swine fever (ASF) is a highly contact infectious disease caused by the African swine fever virus (ASFV). The extremely complex structure and infection mechanism make it difficult to control the spread of ASFV and develop the vaccine. The ASFV genome is huge with many antigenic genes. Among them, CP204L (p30), CP530R (pp62), E183L (p54), B646L (p72), and EP402R (CD2v) are involved in the process of the virus cycle, with strong immunogenicity and the ability to induce the body to produce neutralizing antibodies. In this study, the recombinant virus rBartha-K61-pASFV that expresses the above ASFV antigen genes was constructed by Red/ET recombineering technology using pseudorabies virus (PRV) vaccine strain Bartha-K61. Western blot analysis showed that the ASFV antigen gene was expressed and the recombinant virus showed good genetic stability and proliferation characteristics in 15 continuous generations on porcine kidney (PK15) cells. The results of immunoassay of piglets and mice showed that rBartha-K61-pASFV had good immunogenicity and could induce higher antibody levels in the body. Therefore, PRV was a promising viral vector for expressing the ASFV antigen gene, and all the experiments in this study laid a foundation for the further development of a new viral vector vaccine of ASFV.
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spelling pubmed-90438502022-04-28 Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes Chen, Liyi Zhang, Xinheng Shao, Guanming Shao, Yangyang Hu, Zezhong Feng, Keyu Xie, Zi Li, Hongxin Chen, Weiguo Lin, Wencheng Yuan, Hengxing Wang, Hailong Fu, Jun Xie, Qingmei Front Vet Sci Veterinary Science African swine fever (ASF) is a highly contact infectious disease caused by the African swine fever virus (ASFV). The extremely complex structure and infection mechanism make it difficult to control the spread of ASFV and develop the vaccine. The ASFV genome is huge with many antigenic genes. Among them, CP204L (p30), CP530R (pp62), E183L (p54), B646L (p72), and EP402R (CD2v) are involved in the process of the virus cycle, with strong immunogenicity and the ability to induce the body to produce neutralizing antibodies. In this study, the recombinant virus rBartha-K61-pASFV that expresses the above ASFV antigen genes was constructed by Red/ET recombineering technology using pseudorabies virus (PRV) vaccine strain Bartha-K61. Western blot analysis showed that the ASFV antigen gene was expressed and the recombinant virus showed good genetic stability and proliferation characteristics in 15 continuous generations on porcine kidney (PK15) cells. The results of immunoassay of piglets and mice showed that rBartha-K61-pASFV had good immunogenicity and could induce higher antibody levels in the body. Therefore, PRV was a promising viral vector for expressing the ASFV antigen gene, and all the experiments in this study laid a foundation for the further development of a new viral vector vaccine of ASFV. Frontiers Media S.A. 2022-04-13 /pmc/articles/PMC9043850/ /pubmed/35498728 http://dx.doi.org/10.3389/fvets.2022.832255 Text en Copyright © 2022 Chen, Zhang, Shao, Shao, Hu, Feng, Xie, Li, Chen, Lin, Yuan, Wang, Fu and Xie. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Chen, Liyi
Zhang, Xinheng
Shao, Guanming
Shao, Yangyang
Hu, Zezhong
Feng, Keyu
Xie, Zi
Li, Hongxin
Chen, Weiguo
Lin, Wencheng
Yuan, Hengxing
Wang, Hailong
Fu, Jun
Xie, Qingmei
Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes
title Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes
title_full Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes
title_fullStr Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes
title_full_unstemmed Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes
title_short Construction and Evaluation of Recombinant Pseudorabies Virus Expressing African Swine Fever Virus Antigen Genes
title_sort construction and evaluation of recombinant pseudorabies virus expressing african swine fever virus antigen genes
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043850/
https://www.ncbi.nlm.nih.gov/pubmed/35498728
http://dx.doi.org/10.3389/fvets.2022.832255
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