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Oral Immune Priming Treatment Alters Microbiome Composition in the Red Flour Beetle Tribolium castaneum

It is now well-established that the microbiome is relevant for many of an organism’s properties and that its composition reacts dynamically to various conditions. The microbiome interacts with host immunity and can play important roles in the defenses against pathogens. In invertebrates, immune prim...

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Autores principales: Korša, Ana, Lo, Lai Ka, Gandhi, Shrey, Bang, Corinna, Kurtz, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043903/
https://www.ncbi.nlm.nih.gov/pubmed/35495655
http://dx.doi.org/10.3389/fmicb.2022.793143
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author Korša, Ana
Lo, Lai Ka
Gandhi, Shrey
Bang, Corinna
Kurtz, Joachim
author_facet Korša, Ana
Lo, Lai Ka
Gandhi, Shrey
Bang, Corinna
Kurtz, Joachim
author_sort Korša, Ana
collection PubMed
description It is now well-established that the microbiome is relevant for many of an organism’s properties and that its composition reacts dynamically to various conditions. The microbiome interacts with host immunity and can play important roles in the defenses against pathogens. In invertebrates, immune priming, that is, improved survival upon secondary exposure to a previously encountered pathogen, can be dependent upon the presence of the gut microbiome. However, it is currently unknown whether the microbiome changes upon priming treatment. We here addressed this question in a well-established model for immune priming, the red flour beetle Tribolium castaneum exposed to the entomopathogenic bacterium Bacillus thuringiensis (Bt). After priming treatments, the microbiota composition of beetle larvae was assessed by deep sequencing of the V1-V2 region of the bacterial 16S rRNA gene. We compared the effect of two established routes of priming treatments in this system: injection priming with heat-killed Bt and oral priming via ingestion of filtered sterilized bacterial spore culture supernatants. For oral priming, we used several strains of Bt known to vary in their ability to induce priming. Our study revealed changes in microbiome composition following the oral priming treatment with two different strains of Bt, only one of which (Bt tenebrionis, Btt) is known to lead to improved survival. In contrast, injection priming treatment with the same bacterial strain did not result in microbiome changes. Combined with the previous results indicating that oral priming with Btt depends on the larval microbiome, this suggests that certain members of the microbiome could be involved in forming an oral priming response in the red flour beetle.
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spelling pubmed-90439032022-04-28 Oral Immune Priming Treatment Alters Microbiome Composition in the Red Flour Beetle Tribolium castaneum Korša, Ana Lo, Lai Ka Gandhi, Shrey Bang, Corinna Kurtz, Joachim Front Microbiol Microbiology It is now well-established that the microbiome is relevant for many of an organism’s properties and that its composition reacts dynamically to various conditions. The microbiome interacts with host immunity and can play important roles in the defenses against pathogens. In invertebrates, immune priming, that is, improved survival upon secondary exposure to a previously encountered pathogen, can be dependent upon the presence of the gut microbiome. However, it is currently unknown whether the microbiome changes upon priming treatment. We here addressed this question in a well-established model for immune priming, the red flour beetle Tribolium castaneum exposed to the entomopathogenic bacterium Bacillus thuringiensis (Bt). After priming treatments, the microbiota composition of beetle larvae was assessed by deep sequencing of the V1-V2 region of the bacterial 16S rRNA gene. We compared the effect of two established routes of priming treatments in this system: injection priming with heat-killed Bt and oral priming via ingestion of filtered sterilized bacterial spore culture supernatants. For oral priming, we used several strains of Bt known to vary in their ability to induce priming. Our study revealed changes in microbiome composition following the oral priming treatment with two different strains of Bt, only one of which (Bt tenebrionis, Btt) is known to lead to improved survival. In contrast, injection priming treatment with the same bacterial strain did not result in microbiome changes. Combined with the previous results indicating that oral priming with Btt depends on the larval microbiome, this suggests that certain members of the microbiome could be involved in forming an oral priming response in the red flour beetle. Frontiers Media S.A. 2022-04-13 /pmc/articles/PMC9043903/ /pubmed/35495655 http://dx.doi.org/10.3389/fmicb.2022.793143 Text en Copyright © 2022 Korša, Lo, Gandhi, Bang and Kurtz. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Korša, Ana
Lo, Lai Ka
Gandhi, Shrey
Bang, Corinna
Kurtz, Joachim
Oral Immune Priming Treatment Alters Microbiome Composition in the Red Flour Beetle Tribolium castaneum
title Oral Immune Priming Treatment Alters Microbiome Composition in the Red Flour Beetle Tribolium castaneum
title_full Oral Immune Priming Treatment Alters Microbiome Composition in the Red Flour Beetle Tribolium castaneum
title_fullStr Oral Immune Priming Treatment Alters Microbiome Composition in the Red Flour Beetle Tribolium castaneum
title_full_unstemmed Oral Immune Priming Treatment Alters Microbiome Composition in the Red Flour Beetle Tribolium castaneum
title_short Oral Immune Priming Treatment Alters Microbiome Composition in the Red Flour Beetle Tribolium castaneum
title_sort oral immune priming treatment alters microbiome composition in the red flour beetle tribolium castaneum
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9043903/
https://www.ncbi.nlm.nih.gov/pubmed/35495655
http://dx.doi.org/10.3389/fmicb.2022.793143
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