Cargando…
Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans
Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the ce...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044174/ https://www.ncbi.nlm.nih.gov/pubmed/35498069 http://dx.doi.org/10.1039/d1ra06320k |
_version_ | 1784695046751125504 |
---|---|
author | Mastio, Roberto Willén, Daniel Söderlund, Zackarias Westergren-Thorsson, Gunilla Manner, Sophie Tykesson, Emil Ellervik, Ulf |
author_facet | Mastio, Roberto Willén, Daniel Söderlund, Zackarias Westergren-Thorsson, Gunilla Manner, Sophie Tykesson, Emil Ellervik, Ulf |
author_sort | Mastio, Roberto |
collection | PubMed |
description | Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the cells, but did not enter the Golgi apparatus where most of the glycosaminoglycan biosynthesis occurs. Instead, after a possible double galactosylation by β4GalT7 and β3GalT6, the biosynthesis was terminated. We hypothesize this is due to the charge of the fluorescent probe, which is required for fluorescent ability and stability under physiological conditions. |
format | Online Article Text |
id | pubmed-9044174 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90441742022-04-28 Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans Mastio, Roberto Willén, Daniel Söderlund, Zackarias Westergren-Thorsson, Gunilla Manner, Sophie Tykesson, Emil Ellervik, Ulf RSC Adv Chemistry Five novel xylosides tagged with the fluorescent probe Pacific Blue™ were synthesized and found to act as substrates for β4GalT7, a bottleneck enzyme in the biosynthetic pathways leading to glycosaminoglycans. By confocal microscopy of A549 cells, we showed that the xylosides were taken up by the cells, but did not enter the Golgi apparatus where most of the glycosaminoglycan biosynthesis occurs. Instead, after a possible double galactosylation by β4GalT7 and β3GalT6, the biosynthesis was terminated. We hypothesize this is due to the charge of the fluorescent probe, which is required for fluorescent ability and stability under physiological conditions. The Royal Society of Chemistry 2021-11-29 /pmc/articles/PMC9044174/ /pubmed/35498069 http://dx.doi.org/10.1039/d1ra06320k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Mastio, Roberto Willén, Daniel Söderlund, Zackarias Westergren-Thorsson, Gunilla Manner, Sophie Tykesson, Emil Ellervik, Ulf Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans |
title | Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans |
title_full | Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans |
title_fullStr | Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans |
title_full_unstemmed | Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans |
title_short | Fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans |
title_sort | fluorescently labeled xylosides offer insight into the biosynthetic pathways of glycosaminoglycans |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044174/ https://www.ncbi.nlm.nih.gov/pubmed/35498069 http://dx.doi.org/10.1039/d1ra06320k |
work_keys_str_mv | AT mastioroberto fluorescentlylabeledxylosidesofferinsightintothebiosyntheticpathwaysofglycosaminoglycans AT willendaniel fluorescentlylabeledxylosidesofferinsightintothebiosyntheticpathwaysofglycosaminoglycans AT soderlundzackarias fluorescentlylabeledxylosidesofferinsightintothebiosyntheticpathwaysofglycosaminoglycans AT westergrenthorssongunilla fluorescentlylabeledxylosidesofferinsightintothebiosyntheticpathwaysofglycosaminoglycans AT mannersophie fluorescentlylabeledxylosidesofferinsightintothebiosyntheticpathwaysofglycosaminoglycans AT tykessonemil fluorescentlylabeledxylosidesofferinsightintothebiosyntheticpathwaysofglycosaminoglycans AT ellervikulf fluorescentlylabeledxylosidesofferinsightintothebiosyntheticpathwaysofglycosaminoglycans |