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Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing
This is an unprecedented report of hydrogel gratings with an analyte responsive dye immobilised in alternating strips where the patterned dye is its own dispersive element to perform spectroscopy. At each wavelength, the diffraction efficiency of hydrogel gratings is a function of dye absorbance, wh...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044543/ https://www.ncbi.nlm.nih.gov/pubmed/35494120 http://dx.doi.org/10.1039/d1ra08610c |
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author | Gupta, Ruchi El Sayed, Sameh Goddard, Nicholas J. |
author_facet | Gupta, Ruchi El Sayed, Sameh Goddard, Nicholas J. |
author_sort | Gupta, Ruchi |
collection | PubMed |
description | This is an unprecedented report of hydrogel gratings with an analyte responsive dye immobilised in alternating strips where the patterned dye is its own dispersive element to perform spectroscopy. At each wavelength, the diffraction efficiency of hydrogel gratings is a function of dye absorbance, which in turn is dependent on the concentration of analytes in samples. Thus, changes in intensity of diffracted light of hydrogel gratings were measured for sensing of analytes. Equally, the ratio of diffracted intensities at two wavelengths was used for quantification of analytes to reduce errors caused by variations in intensity of light sources and photobleaching of dyes. 15.27 μm pitch gratings were fabricated by exposing 175 μm thick films of photofunctionalisable poly(acrylamide) hydrogel in a laser interferometric lithography setup, generating an array of alternating lines with and without free functional groups. The freed functional groups were reacted with pH sensitive fluorescein isothiocyanate to create gratings for measurement of pH. The ratio of intensity of diffracted light of hydrogel gratings at 430 and 475 nm was shown to be linear over 4 pH units, which compares favourably with ∼2 pH units for conventional absorption spectroscopy. This increased dynamic range was a result of cancellation of the opposite non-linearities in the pH response of the analyte responsive dye and the diffraction efficiency as a function of dye absorbance. |
format | Online Article Text |
id | pubmed-9044543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90445432022-04-28 Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing Gupta, Ruchi El Sayed, Sameh Goddard, Nicholas J. RSC Adv Chemistry This is an unprecedented report of hydrogel gratings with an analyte responsive dye immobilised in alternating strips where the patterned dye is its own dispersive element to perform spectroscopy. At each wavelength, the diffraction efficiency of hydrogel gratings is a function of dye absorbance, which in turn is dependent on the concentration of analytes in samples. Thus, changes in intensity of diffracted light of hydrogel gratings were measured for sensing of analytes. Equally, the ratio of diffracted intensities at two wavelengths was used for quantification of analytes to reduce errors caused by variations in intensity of light sources and photobleaching of dyes. 15.27 μm pitch gratings were fabricated by exposing 175 μm thick films of photofunctionalisable poly(acrylamide) hydrogel in a laser interferometric lithography setup, generating an array of alternating lines with and without free functional groups. The freed functional groups were reacted with pH sensitive fluorescein isothiocyanate to create gratings for measurement of pH. The ratio of intensity of diffracted light of hydrogel gratings at 430 and 475 nm was shown to be linear over 4 pH units, which compares favourably with ∼2 pH units for conventional absorption spectroscopy. This increased dynamic range was a result of cancellation of the opposite non-linearities in the pH response of the analyte responsive dye and the diffraction efficiency as a function of dye absorbance. The Royal Society of Chemistry 2021-12-17 /pmc/articles/PMC9044543/ /pubmed/35494120 http://dx.doi.org/10.1039/d1ra08610c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Gupta, Ruchi El Sayed, Sameh Goddard, Nicholas J. Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing |
title | Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing |
title_full | Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing |
title_fullStr | Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing |
title_full_unstemmed | Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing |
title_short | Hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing |
title_sort | hydrogel gratings with patterned analyte responsive dyes for spectroscopic sensing |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044543/ https://www.ncbi.nlm.nih.gov/pubmed/35494120 http://dx.doi.org/10.1039/d1ra08610c |
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