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Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography
Whole-block imaging (WBI) using micro-computed tomography (micro-CT) allows the nondestructive reconstruction of a three-dimensional view of tissues, implying that WBI may be used for accurate pathological evaluation of patients with rectal cancer. HOWEVER, the clinical impact of this approach is un...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044748/ https://www.ncbi.nlm.nih.gov/pubmed/35454033 http://dx.doi.org/10.3390/diagnostics12040984 |
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author | Yoshida, Masao Cesmecioglu, Emine Firat, Canan Sakamoto, Hirotsugu Teplov, Alexei Kawata, Noboru Ntiamoah, Peter Ohnishi, Takashi Ibrahim, Kareem Vakiani, Efsevia Garcia-Aguilar, Julio Hameed, Meera Shia, Jinru Yagi, Yukako |
author_facet | Yoshida, Masao Cesmecioglu, Emine Firat, Canan Sakamoto, Hirotsugu Teplov, Alexei Kawata, Noboru Ntiamoah, Peter Ohnishi, Takashi Ibrahim, Kareem Vakiani, Efsevia Garcia-Aguilar, Julio Hameed, Meera Shia, Jinru Yagi, Yukako |
author_sort | Yoshida, Masao |
collection | PubMed |
description | Whole-block imaging (WBI) using micro-computed tomography (micro-CT) allows the nondestructive reconstruction of a three-dimensional view of tissues, implying that WBI may be used for accurate pathological evaluation of patients with rectal cancer. HOWEVER, the clinical impact of this approach is unclear. We aimed to clarify the efficacy of WBI in the whole-mount specimens of locally advanced rectal cancer. A total of 237 whole-mount formalin-fixed paraffin-embedded blocks from 13 patients with rectal cancer who underwent surgical treatment were enrolled and scanned with micro-CT to generate three-dimensional images. WBI was evaluated following the conventional pathological review of the corresponding whole-slide imaging (WSI). WBI identified all tumor sites detected using WSI. Furthermore, WBI revealed one additional tumor site, which was not detected using WSI. Tumor resection margin was significantly closer to the soft-tissue edge when measured using WBI (7.7 mm vs. 6.6 mm, p < 0.01). Seventy-six percent of tumor deposits on WSI were changed according to the evidence of tumor interaction with the surrounding tissues confirmed using WBI. Furthermore, WBI revealed 25 additional lymph nodes, six of which were metastatic. The combination of conventional hematoxylin and eosin-stained imaging and WBI may contribute to an accurate pathological assessment. |
format | Online Article Text |
id | pubmed-9044748 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-90447482022-04-28 Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography Yoshida, Masao Cesmecioglu, Emine Firat, Canan Sakamoto, Hirotsugu Teplov, Alexei Kawata, Noboru Ntiamoah, Peter Ohnishi, Takashi Ibrahim, Kareem Vakiani, Efsevia Garcia-Aguilar, Julio Hameed, Meera Shia, Jinru Yagi, Yukako Diagnostics (Basel) Article Whole-block imaging (WBI) using micro-computed tomography (micro-CT) allows the nondestructive reconstruction of a three-dimensional view of tissues, implying that WBI may be used for accurate pathological evaluation of patients with rectal cancer. HOWEVER, the clinical impact of this approach is unclear. We aimed to clarify the efficacy of WBI in the whole-mount specimens of locally advanced rectal cancer. A total of 237 whole-mount formalin-fixed paraffin-embedded blocks from 13 patients with rectal cancer who underwent surgical treatment were enrolled and scanned with micro-CT to generate three-dimensional images. WBI was evaluated following the conventional pathological review of the corresponding whole-slide imaging (WSI). WBI identified all tumor sites detected using WSI. Furthermore, WBI revealed one additional tumor site, which was not detected using WSI. Tumor resection margin was significantly closer to the soft-tissue edge when measured using WBI (7.7 mm vs. 6.6 mm, p < 0.01). Seventy-six percent of tumor deposits on WSI were changed according to the evidence of tumor interaction with the surrounding tissues confirmed using WBI. Furthermore, WBI revealed 25 additional lymph nodes, six of which were metastatic. The combination of conventional hematoxylin and eosin-stained imaging and WBI may contribute to an accurate pathological assessment. MDPI 2022-04-14 /pmc/articles/PMC9044748/ /pubmed/35454033 http://dx.doi.org/10.3390/diagnostics12040984 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yoshida, Masao Cesmecioglu, Emine Firat, Canan Sakamoto, Hirotsugu Teplov, Alexei Kawata, Noboru Ntiamoah, Peter Ohnishi, Takashi Ibrahim, Kareem Vakiani, Efsevia Garcia-Aguilar, Julio Hameed, Meera Shia, Jinru Yagi, Yukako Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography |
title | Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography |
title_full | Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography |
title_fullStr | Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography |
title_full_unstemmed | Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography |
title_short | Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography |
title_sort | pathological evaluation of rectal cancer specimens using micro-computed tomography |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044748/ https://www.ncbi.nlm.nih.gov/pubmed/35454033 http://dx.doi.org/10.3390/diagnostics12040984 |
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