Cargando…

Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography

Whole-block imaging (WBI) using micro-computed tomography (micro-CT) allows the nondestructive reconstruction of a three-dimensional view of tissues, implying that WBI may be used for accurate pathological evaluation of patients with rectal cancer. HOWEVER, the clinical impact of this approach is un...

Descripción completa

Detalles Bibliográficos
Autores principales: Yoshida, Masao, Cesmecioglu, Emine, Firat, Canan, Sakamoto, Hirotsugu, Teplov, Alexei, Kawata, Noboru, Ntiamoah, Peter, Ohnishi, Takashi, Ibrahim, Kareem, Vakiani, Efsevia, Garcia-Aguilar, Julio, Hameed, Meera, Shia, Jinru, Yagi, Yukako
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044748/
https://www.ncbi.nlm.nih.gov/pubmed/35454033
http://dx.doi.org/10.3390/diagnostics12040984
_version_ 1784695171233873920
author Yoshida, Masao
Cesmecioglu, Emine
Firat, Canan
Sakamoto, Hirotsugu
Teplov, Alexei
Kawata, Noboru
Ntiamoah, Peter
Ohnishi, Takashi
Ibrahim, Kareem
Vakiani, Efsevia
Garcia-Aguilar, Julio
Hameed, Meera
Shia, Jinru
Yagi, Yukako
author_facet Yoshida, Masao
Cesmecioglu, Emine
Firat, Canan
Sakamoto, Hirotsugu
Teplov, Alexei
Kawata, Noboru
Ntiamoah, Peter
Ohnishi, Takashi
Ibrahim, Kareem
Vakiani, Efsevia
Garcia-Aguilar, Julio
Hameed, Meera
Shia, Jinru
Yagi, Yukako
author_sort Yoshida, Masao
collection PubMed
description Whole-block imaging (WBI) using micro-computed tomography (micro-CT) allows the nondestructive reconstruction of a three-dimensional view of tissues, implying that WBI may be used for accurate pathological evaluation of patients with rectal cancer. HOWEVER, the clinical impact of this approach is unclear. We aimed to clarify the efficacy of WBI in the whole-mount specimens of locally advanced rectal cancer. A total of 237 whole-mount formalin-fixed paraffin-embedded blocks from 13 patients with rectal cancer who underwent surgical treatment were enrolled and scanned with micro-CT to generate three-dimensional images. WBI was evaluated following the conventional pathological review of the corresponding whole-slide imaging (WSI). WBI identified all tumor sites detected using WSI. Furthermore, WBI revealed one additional tumor site, which was not detected using WSI. Tumor resection margin was significantly closer to the soft-tissue edge when measured using WBI (7.7 mm vs. 6.6 mm, p < 0.01). Seventy-six percent of tumor deposits on WSI were changed according to the evidence of tumor interaction with the surrounding tissues confirmed using WBI. Furthermore, WBI revealed 25 additional lymph nodes, six of which were metastatic. The combination of conventional hematoxylin and eosin-stained imaging and WBI may contribute to an accurate pathological assessment.
format Online
Article
Text
id pubmed-9044748
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-90447482022-04-28 Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography Yoshida, Masao Cesmecioglu, Emine Firat, Canan Sakamoto, Hirotsugu Teplov, Alexei Kawata, Noboru Ntiamoah, Peter Ohnishi, Takashi Ibrahim, Kareem Vakiani, Efsevia Garcia-Aguilar, Julio Hameed, Meera Shia, Jinru Yagi, Yukako Diagnostics (Basel) Article Whole-block imaging (WBI) using micro-computed tomography (micro-CT) allows the nondestructive reconstruction of a three-dimensional view of tissues, implying that WBI may be used for accurate pathological evaluation of patients with rectal cancer. HOWEVER, the clinical impact of this approach is unclear. We aimed to clarify the efficacy of WBI in the whole-mount specimens of locally advanced rectal cancer. A total of 237 whole-mount formalin-fixed paraffin-embedded blocks from 13 patients with rectal cancer who underwent surgical treatment were enrolled and scanned with micro-CT to generate three-dimensional images. WBI was evaluated following the conventional pathological review of the corresponding whole-slide imaging (WSI). WBI identified all tumor sites detected using WSI. Furthermore, WBI revealed one additional tumor site, which was not detected using WSI. Tumor resection margin was significantly closer to the soft-tissue edge when measured using WBI (7.7 mm vs. 6.6 mm, p < 0.01). Seventy-six percent of tumor deposits on WSI were changed according to the evidence of tumor interaction with the surrounding tissues confirmed using WBI. Furthermore, WBI revealed 25 additional lymph nodes, six of which were metastatic. The combination of conventional hematoxylin and eosin-stained imaging and WBI may contribute to an accurate pathological assessment. MDPI 2022-04-14 /pmc/articles/PMC9044748/ /pubmed/35454033 http://dx.doi.org/10.3390/diagnostics12040984 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yoshida, Masao
Cesmecioglu, Emine
Firat, Canan
Sakamoto, Hirotsugu
Teplov, Alexei
Kawata, Noboru
Ntiamoah, Peter
Ohnishi, Takashi
Ibrahim, Kareem
Vakiani, Efsevia
Garcia-Aguilar, Julio
Hameed, Meera
Shia, Jinru
Yagi, Yukako
Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography
title Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography
title_full Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography
title_fullStr Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography
title_full_unstemmed Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography
title_short Pathological Evaluation of Rectal Cancer Specimens Using Micro-Computed Tomography
title_sort pathological evaluation of rectal cancer specimens using micro-computed tomography
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044748/
https://www.ncbi.nlm.nih.gov/pubmed/35454033
http://dx.doi.org/10.3390/diagnostics12040984
work_keys_str_mv AT yoshidamasao pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT cesmeciogluemine pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT firatcanan pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT sakamotohirotsugu pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT teplovalexei pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT kawatanoboru pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT ntiamoahpeter pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT ohnishitakashi pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT ibrahimkareem pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT vakianiefsevia pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT garciaaguilarjulio pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT hameedmeera pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT shiajinru pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography
AT yagiyukako pathologicalevaluationofrectalcancerspecimensusingmicrocomputedtomography