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High expression of RIPK2 is associated with Taxol resistance in serous ovarian cancer

BACKGROUND: Taxol resistance in serous ovarian cancer is responsible for its poor prognosis, yet the underlying mechanism is still poorly understood. Thus, we probed the mechanism of Taxol resistance in serous ovarian cancer with multiple bioinformatic methods to provide novel insights into potentia...

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Autores principales: Shen, Yuqing, Lin, Hui, Chen, Kelie, Ge, Wanzhong, Xia, Dajing, Wu, Yihua, Lu, Weiguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044796/
https://www.ncbi.nlm.nih.gov/pubmed/35477477
http://dx.doi.org/10.1186/s13048-022-00986-2
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author Shen, Yuqing
Lin, Hui
Chen, Kelie
Ge, Wanzhong
Xia, Dajing
Wu, Yihua
Lu, Weiguo
author_facet Shen, Yuqing
Lin, Hui
Chen, Kelie
Ge, Wanzhong
Xia, Dajing
Wu, Yihua
Lu, Weiguo
author_sort Shen, Yuqing
collection PubMed
description BACKGROUND: Taxol resistance in serous ovarian cancer is responsible for its poor prognosis, yet the underlying mechanism is still poorly understood. Thus, we probed the mechanism of Taxol resistance in serous ovarian cancer with multiple bioinformatic methods to provide novel insights into potential therapies. METHODS: The differentially expressed genes (DEGs) in Taxol-sensitive and Taxol-resistant cell lines and their relationship with the overall survival (OS) and progression-free interval (PFI) of ovarian cancer patients were analyzed using gene expression datasets from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). The role of receptor interacting serine/threonine kinase 2 (RIPK2) was validated via identification of its coexpressed genes, functional analysis and generation of a protein-protein interaction (PPI) network. The single sample gene set enrichment analysis (ssGSEA) was used to explore immune infiltration, and genomic alterations of RIPK2 were also analyzed via cBio Cancer Genomics Portal (cBioProtal). RESULTS: RIPK2 was highly expressed in Taxol resistant ovarian cancer cell lines, and its high expression was also linked with shorter OS and PFI in serous ovarian cancer patients. The PPI network analysis and pathway analysis demonstrated that RIPK2 might participate in the positive regulation of NF-κB transcription factor activity. RIPK2 expression was related to tumor microenvironment alterations, which might participate in the formation of Taxol resistance. CONCLUSIONS: Our studies suggested that high expression of RIPK2 is related to Taxol resistance in serous ovarian cancer, and that RIPK2 induces Taxol resistance through NOD1/RIPK2/NF-κB inflammatory pathway activation and tumor microenvironment changes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13048-022-00986-2.
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spelling pubmed-90447962022-04-28 High expression of RIPK2 is associated with Taxol resistance in serous ovarian cancer Shen, Yuqing Lin, Hui Chen, Kelie Ge, Wanzhong Xia, Dajing Wu, Yihua Lu, Weiguo J Ovarian Res Research BACKGROUND: Taxol resistance in serous ovarian cancer is responsible for its poor prognosis, yet the underlying mechanism is still poorly understood. Thus, we probed the mechanism of Taxol resistance in serous ovarian cancer with multiple bioinformatic methods to provide novel insights into potential therapies. METHODS: The differentially expressed genes (DEGs) in Taxol-sensitive and Taxol-resistant cell lines and their relationship with the overall survival (OS) and progression-free interval (PFI) of ovarian cancer patients were analyzed using gene expression datasets from the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). The role of receptor interacting serine/threonine kinase 2 (RIPK2) was validated via identification of its coexpressed genes, functional analysis and generation of a protein-protein interaction (PPI) network. The single sample gene set enrichment analysis (ssGSEA) was used to explore immune infiltration, and genomic alterations of RIPK2 were also analyzed via cBio Cancer Genomics Portal (cBioProtal). RESULTS: RIPK2 was highly expressed in Taxol resistant ovarian cancer cell lines, and its high expression was also linked with shorter OS and PFI in serous ovarian cancer patients. The PPI network analysis and pathway analysis demonstrated that RIPK2 might participate in the positive regulation of NF-κB transcription factor activity. RIPK2 expression was related to tumor microenvironment alterations, which might participate in the formation of Taxol resistance. CONCLUSIONS: Our studies suggested that high expression of RIPK2 is related to Taxol resistance in serous ovarian cancer, and that RIPK2 induces Taxol resistance through NOD1/RIPK2/NF-κB inflammatory pathway activation and tumor microenvironment changes. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13048-022-00986-2. BioMed Central 2022-04-27 /pmc/articles/PMC9044796/ /pubmed/35477477 http://dx.doi.org/10.1186/s13048-022-00986-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visithttp://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Shen, Yuqing
Lin, Hui
Chen, Kelie
Ge, Wanzhong
Xia, Dajing
Wu, Yihua
Lu, Weiguo
High expression of RIPK2 is associated with Taxol resistance in serous ovarian cancer
title High expression of RIPK2 is associated with Taxol resistance in serous ovarian cancer
title_full High expression of RIPK2 is associated with Taxol resistance in serous ovarian cancer
title_fullStr High expression of RIPK2 is associated with Taxol resistance in serous ovarian cancer
title_full_unstemmed High expression of RIPK2 is associated with Taxol resistance in serous ovarian cancer
title_short High expression of RIPK2 is associated with Taxol resistance in serous ovarian cancer
title_sort high expression of ripk2 is associated with taxol resistance in serous ovarian cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9044796/
https://www.ncbi.nlm.nih.gov/pubmed/35477477
http://dx.doi.org/10.1186/s13048-022-00986-2
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