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Ovarian granulosa cells from women with PCOS express low levels of SARS-CoV-2 receptors and co-factors
PURPOSE: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is global pandemic with more than 5 million deaths so far. Female reproductive tract organs express coronavirus-associated receptors and factors (SCARFs), suggesting they may be susceptible to SARS-CoV-2 infection; howev...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045021/ https://www.ncbi.nlm.nih.gov/pubmed/35477803 http://dx.doi.org/10.1007/s00404-022-06567-4 |
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author | Naigaonkar, Aalaap Patil, Krutika Joseph, Shaini Hinduja, Indira Mukherjee, Srabani |
author_facet | Naigaonkar, Aalaap Patil, Krutika Joseph, Shaini Hinduja, Indira Mukherjee, Srabani |
author_sort | Naigaonkar, Aalaap |
collection | PubMed |
description | PURPOSE: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is global pandemic with more than 5 million deaths so far. Female reproductive tract organs express coronavirus-associated receptors and factors (SCARFs), suggesting they may be susceptible to SARS-CoV-2 infection; however, the susceptibility of ovary/follicle/oocyte to the same is still elusive. Co-morbidities like obesity, type-2 diabetes mellitus, cardiovascular disease, etc. increase the risk of SARS-CoV-2 infection. These features are common in women with polycystic ovary syndrome (PCOS), warranting further scope to study SCARFs expression in ovary of these women. MATERIALS AND METHODS: SCARFs expression in ovary and ovarian tissues of women with PCOS and healthy women was explored by analyzing publically available microarray datasets. Transcript expressions of SCARFs were investigated in mural and cumulus granulosa cells (MGCs and CGCs) from control and PCOS women undergoing in vitro fertilization (IVF). RESULTS: Microarray data revealed that ovary expresses all genes necessary for SARS-CoV-2 infection. PCOS women mostly showed down-regulated/unchanged levels of SCARFs. MGCs and CGCs from PCOS women showed lower expression of receptors ACE2, BSG and DPP4 and protease CTSB than in controls. MGCs showed lower expression of protease CTSL in PCOS than in controls. Expression of TMPRSS2 was not detected in both cell types. CONCLUSION: Human ovarian follicle may be susceptible to SARS-CoV-2 infection. Lower expression of SCARFs in PCOS indicates that the risk of SARS-CoV-2 infection to the ovary may be lesser in these women than controls. This knowledge may help in safe practices at IVF settings in the current pandemic. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00404-022-06567-4. |
format | Online Article Text |
id | pubmed-9045021 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-90450212022-04-28 Ovarian granulosa cells from women with PCOS express low levels of SARS-CoV-2 receptors and co-factors Naigaonkar, Aalaap Patil, Krutika Joseph, Shaini Hinduja, Indira Mukherjee, Srabani Arch Gynecol Obstet Gynecologic Endocrinology and Reproductive Medicine PURPOSE: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is global pandemic with more than 5 million deaths so far. Female reproductive tract organs express coronavirus-associated receptors and factors (SCARFs), suggesting they may be susceptible to SARS-CoV-2 infection; however, the susceptibility of ovary/follicle/oocyte to the same is still elusive. Co-morbidities like obesity, type-2 diabetes mellitus, cardiovascular disease, etc. increase the risk of SARS-CoV-2 infection. These features are common in women with polycystic ovary syndrome (PCOS), warranting further scope to study SCARFs expression in ovary of these women. MATERIALS AND METHODS: SCARFs expression in ovary and ovarian tissues of women with PCOS and healthy women was explored by analyzing publically available microarray datasets. Transcript expressions of SCARFs were investigated in mural and cumulus granulosa cells (MGCs and CGCs) from control and PCOS women undergoing in vitro fertilization (IVF). RESULTS: Microarray data revealed that ovary expresses all genes necessary for SARS-CoV-2 infection. PCOS women mostly showed down-regulated/unchanged levels of SCARFs. MGCs and CGCs from PCOS women showed lower expression of receptors ACE2, BSG and DPP4 and protease CTSB than in controls. MGCs showed lower expression of protease CTSL in PCOS than in controls. Expression of TMPRSS2 was not detected in both cell types. CONCLUSION: Human ovarian follicle may be susceptible to SARS-CoV-2 infection. Lower expression of SCARFs in PCOS indicates that the risk of SARS-CoV-2 infection to the ovary may be lesser in these women than controls. This knowledge may help in safe practices at IVF settings in the current pandemic. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00404-022-06567-4. Springer Berlin Heidelberg 2022-04-27 2022 /pmc/articles/PMC9045021/ /pubmed/35477803 http://dx.doi.org/10.1007/s00404-022-06567-4 Text en © The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Gynecologic Endocrinology and Reproductive Medicine Naigaonkar, Aalaap Patil, Krutika Joseph, Shaini Hinduja, Indira Mukherjee, Srabani Ovarian granulosa cells from women with PCOS express low levels of SARS-CoV-2 receptors and co-factors |
title | Ovarian granulosa cells from women with PCOS express low levels of SARS-CoV-2 receptors and co-factors |
title_full | Ovarian granulosa cells from women with PCOS express low levels of SARS-CoV-2 receptors and co-factors |
title_fullStr | Ovarian granulosa cells from women with PCOS express low levels of SARS-CoV-2 receptors and co-factors |
title_full_unstemmed | Ovarian granulosa cells from women with PCOS express low levels of SARS-CoV-2 receptors and co-factors |
title_short | Ovarian granulosa cells from women with PCOS express low levels of SARS-CoV-2 receptors and co-factors |
title_sort | ovarian granulosa cells from women with pcos express low levels of sars-cov-2 receptors and co-factors |
topic | Gynecologic Endocrinology and Reproductive Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045021/ https://www.ncbi.nlm.nih.gov/pubmed/35477803 http://dx.doi.org/10.1007/s00404-022-06567-4 |
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