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Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program

Bioactive materials harness the body’s innate regenerative potential by directing endogenous progenitor cells to facilitate tissue repair. Dissolution products of inorganic biomaterials provide unique biomolecular signaling for tissue-specific differentiation. Inorganic ions (minerals) are vital to...

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Autores principales: Brokesh, Anna M., Cross, Lauren M., Kersey, Anna L., Murali, Aparna, Richter, Christopher, Gregory, Carl A., Singh, Irtisha, Gaharwar, Akhilesh K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045714/
https://www.ncbi.nlm.nih.gov/pubmed/35476448
http://dx.doi.org/10.1126/sciadv.abl9404
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author Brokesh, Anna M.
Cross, Lauren M.
Kersey, Anna L.
Murali, Aparna
Richter, Christopher
Gregory, Carl A.
Singh, Irtisha
Gaharwar, Akhilesh K.
author_facet Brokesh, Anna M.
Cross, Lauren M.
Kersey, Anna L.
Murali, Aparna
Richter, Christopher
Gregory, Carl A.
Singh, Irtisha
Gaharwar, Akhilesh K.
author_sort Brokesh, Anna M.
collection PubMed
description Bioactive materials harness the body’s innate regenerative potential by directing endogenous progenitor cells to facilitate tissue repair. Dissolution products of inorganic biomaterials provide unique biomolecular signaling for tissue-specific differentiation. Inorganic ions (minerals) are vital to biological processes and play crucial roles in regulating gene expression patterns and directing cellular fate. However, mechanisms by which ionic dissolution products affect cellular differentiation are not well characterized. We demonstrate the role of the inorganic biomaterial synthetic two-dimensional nanosilicates and its ionic dissolution products on human mesenchymal stem cell differentiation. We use whole-transcriptome sequencing (RNA-sequencing) to characterize the contribution of nanosilicates and its ionic dissolution products on endochondral differentiation. Our study highlights the modulatory role of ions in stem cell transcriptome dynamics by regulating lineage-specific gene expression patterns. This work paves the way for leveraging biochemical characteristics of inorganic biomaterials to direct cellular processes and promote in situ tissue regeneration.
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spelling pubmed-90457142022-05-04 Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program Brokesh, Anna M. Cross, Lauren M. Kersey, Anna L. Murali, Aparna Richter, Christopher Gregory, Carl A. Singh, Irtisha Gaharwar, Akhilesh K. Sci Adv Biomedicine and Life Sciences Bioactive materials harness the body’s innate regenerative potential by directing endogenous progenitor cells to facilitate tissue repair. Dissolution products of inorganic biomaterials provide unique biomolecular signaling for tissue-specific differentiation. Inorganic ions (minerals) are vital to biological processes and play crucial roles in regulating gene expression patterns and directing cellular fate. However, mechanisms by which ionic dissolution products affect cellular differentiation are not well characterized. We demonstrate the role of the inorganic biomaterial synthetic two-dimensional nanosilicates and its ionic dissolution products on human mesenchymal stem cell differentiation. We use whole-transcriptome sequencing (RNA-sequencing) to characterize the contribution of nanosilicates and its ionic dissolution products on endochondral differentiation. Our study highlights the modulatory role of ions in stem cell transcriptome dynamics by regulating lineage-specific gene expression patterns. This work paves the way for leveraging biochemical characteristics of inorganic biomaterials to direct cellular processes and promote in situ tissue regeneration. American Association for the Advancement of Science 2022-04-27 /pmc/articles/PMC9045714/ /pubmed/35476448 http://dx.doi.org/10.1126/sciadv.abl9404 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Brokesh, Anna M.
Cross, Lauren M.
Kersey, Anna L.
Murali, Aparna
Richter, Christopher
Gregory, Carl A.
Singh, Irtisha
Gaharwar, Akhilesh K.
Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program
title Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program
title_full Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program
title_fullStr Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program
title_full_unstemmed Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program
title_short Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program
title_sort dissociation of nanosilicates induces downstream endochondral differentiation gene expression program
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045714/
https://www.ncbi.nlm.nih.gov/pubmed/35476448
http://dx.doi.org/10.1126/sciadv.abl9404
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