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Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program
Bioactive materials harness the body’s innate regenerative potential by directing endogenous progenitor cells to facilitate tissue repair. Dissolution products of inorganic biomaterials provide unique biomolecular signaling for tissue-specific differentiation. Inorganic ions (minerals) are vital to...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045714/ https://www.ncbi.nlm.nih.gov/pubmed/35476448 http://dx.doi.org/10.1126/sciadv.abl9404 |
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author | Brokesh, Anna M. Cross, Lauren M. Kersey, Anna L. Murali, Aparna Richter, Christopher Gregory, Carl A. Singh, Irtisha Gaharwar, Akhilesh K. |
author_facet | Brokesh, Anna M. Cross, Lauren M. Kersey, Anna L. Murali, Aparna Richter, Christopher Gregory, Carl A. Singh, Irtisha Gaharwar, Akhilesh K. |
author_sort | Brokesh, Anna M. |
collection | PubMed |
description | Bioactive materials harness the body’s innate regenerative potential by directing endogenous progenitor cells to facilitate tissue repair. Dissolution products of inorganic biomaterials provide unique biomolecular signaling for tissue-specific differentiation. Inorganic ions (minerals) are vital to biological processes and play crucial roles in regulating gene expression patterns and directing cellular fate. However, mechanisms by which ionic dissolution products affect cellular differentiation are not well characterized. We demonstrate the role of the inorganic biomaterial synthetic two-dimensional nanosilicates and its ionic dissolution products on human mesenchymal stem cell differentiation. We use whole-transcriptome sequencing (RNA-sequencing) to characterize the contribution of nanosilicates and its ionic dissolution products on endochondral differentiation. Our study highlights the modulatory role of ions in stem cell transcriptome dynamics by regulating lineage-specific gene expression patterns. This work paves the way for leveraging biochemical characteristics of inorganic biomaterials to direct cellular processes and promote in situ tissue regeneration. |
format | Online Article Text |
id | pubmed-9045714 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-90457142022-05-04 Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program Brokesh, Anna M. Cross, Lauren M. Kersey, Anna L. Murali, Aparna Richter, Christopher Gregory, Carl A. Singh, Irtisha Gaharwar, Akhilesh K. Sci Adv Biomedicine and Life Sciences Bioactive materials harness the body’s innate regenerative potential by directing endogenous progenitor cells to facilitate tissue repair. Dissolution products of inorganic biomaterials provide unique biomolecular signaling for tissue-specific differentiation. Inorganic ions (minerals) are vital to biological processes and play crucial roles in regulating gene expression patterns and directing cellular fate. However, mechanisms by which ionic dissolution products affect cellular differentiation are not well characterized. We demonstrate the role of the inorganic biomaterial synthetic two-dimensional nanosilicates and its ionic dissolution products on human mesenchymal stem cell differentiation. We use whole-transcriptome sequencing (RNA-sequencing) to characterize the contribution of nanosilicates and its ionic dissolution products on endochondral differentiation. Our study highlights the modulatory role of ions in stem cell transcriptome dynamics by regulating lineage-specific gene expression patterns. This work paves the way for leveraging biochemical characteristics of inorganic biomaterials to direct cellular processes and promote in situ tissue regeneration. American Association for the Advancement of Science 2022-04-27 /pmc/articles/PMC9045714/ /pubmed/35476448 http://dx.doi.org/10.1126/sciadv.abl9404 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Biomedicine and Life Sciences Brokesh, Anna M. Cross, Lauren M. Kersey, Anna L. Murali, Aparna Richter, Christopher Gregory, Carl A. Singh, Irtisha Gaharwar, Akhilesh K. Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program |
title | Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program |
title_full | Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program |
title_fullStr | Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program |
title_full_unstemmed | Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program |
title_short | Dissociation of nanosilicates induces downstream endochondral differentiation gene expression program |
title_sort | dissociation of nanosilicates induces downstream endochondral differentiation gene expression program |
topic | Biomedicine and Life Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9045714/ https://www.ncbi.nlm.nih.gov/pubmed/35476448 http://dx.doi.org/10.1126/sciadv.abl9404 |
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