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BTB(BCL6) dimers as building blocks for reversible drug-induced protein oligomerization
Here, we characterize the BTB domain of the transcription factor BCL6 (BTB(BCL6)) as a small-molecule-controlled, reversible oligomerization switch, which oligomerizes upon BI-3802 treatment and de-oligomerizes upon addition of BI-3812. We show that the magnitude of oligomerization can be controlled...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046236/ https://www.ncbi.nlm.nih.gov/pubmed/35497498 http://dx.doi.org/10.1016/j.crmeth.2022.100193 |
Sumario: | Here, we characterize the BTB domain of the transcription factor BCL6 (BTB(BCL6)) as a small-molecule-controlled, reversible oligomerization switch, which oligomerizes upon BI-3802 treatment and de-oligomerizes upon addition of BI-3812. We show that the magnitude of oligomerization can be controlled in vitro by BI-3802 concentration and exposure time. In cellular models, exposure to BI-3802/BI-3812 can drive multiple cycles of foci formation consisting of BTB(BCL6) fused to EGFP, which are not degraded due to the lack of a degron. We generated an epidermal growth factor receptor (EGFR)-BTB(BCL6) fusion. Treatment with BI-3802, as an ON switch, induced EGFR-BTB(BCL6) phosphorylation and activation of downstream effectors, which could in part be reversed by the addition of BI-3812, as an OFF switch. Finally, BI-3802-induced oligomerization of the EGFR-BTB(BCL6) fusion enhanced proliferation of an EGF-dependent cell line in absence of EGF. These results demonstrate the successful application of small-molecule-induced, reversible oligomerization as a switch for synthetic biology. |
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