Quantification of tissue-specific protein translation in whole C. elegans using O-propargyl-puromycin labeling and fluorescence microscopy

The regulation of gene expression via protein translation is critical for growth, development, and stress response. While puromycin-based techniques have been used to quantify protein translation in C. elegans, they have been limited to using lysate from whole worms. To achieve tissue-specific quant...

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Detalles Bibliográficos
Autores principales: Somers, Hannah M., Fuqua, Jeremy H., Bonnet, Frédéric X.A., Rollins, Jarod A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046455/
https://www.ncbi.nlm.nih.gov/pubmed/35497499
http://dx.doi.org/10.1016/j.crmeth.2022.100203

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046455/
https://www.ncbi.nlm.nih.gov/pubmed/35497499
http://dx.doi.org/10.1016/j.crmeth.2022.100203

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