Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms

In this protocol, we describe a germ-free Drosophila melanogaster model to investigate anaerobic bacterial biofilms. We detail how to establish Propionibacterium spp. biofilms in the fruit fly’s gut using an easy to carry out method. For complete details on the use and execution of this protocol, pl...

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Detalles Bibliográficos
Autores principales: Bronnec, Vicky, Alexeyev, Oleg A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046622/
https://www.ncbi.nlm.nih.gov/pubmed/35496790
http://dx.doi.org/10.1016/j.xpro.2022.101342
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author Bronnec, Vicky
Alexeyev, Oleg A.
author_facet Bronnec, Vicky
Alexeyev, Oleg A.
author_sort Bronnec, Vicky
collection PubMed
description In this protocol, we describe a germ-free Drosophila melanogaster model to investigate anaerobic bacterial biofilms. We detail how to establish Propionibacterium spp. biofilms in the fruit fly’s gut using an easy to carry out method. For complete details on the use and execution of this protocol, please refer to Bronnec and Alexeyev (2021) and Bronnec et al. (2022).
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spelling pubmed-90466222022-04-29 Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms Bronnec, Vicky Alexeyev, Oleg A. STAR Protoc Protocol In this protocol, we describe a germ-free Drosophila melanogaster model to investigate anaerobic bacterial biofilms. We detail how to establish Propionibacterium spp. biofilms in the fruit fly’s gut using an easy to carry out method. For complete details on the use and execution of this protocol, please refer to Bronnec and Alexeyev (2021) and Bronnec et al. (2022). Elsevier 2022-04-22 /pmc/articles/PMC9046622/ /pubmed/35496790 http://dx.doi.org/10.1016/j.xpro.2022.101342 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Bronnec, Vicky
Alexeyev, Oleg A.
Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms
title Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms
title_full Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms
title_fullStr Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms
title_full_unstemmed Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms
title_short Detailed protocol for germ-free Drosophila melanogaster colonization with Propionibacterium spp. biofilms
title_sort detailed protocol for germ-free drosophila melanogaster colonization with propionibacterium spp. biofilms
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046622/
https://www.ncbi.nlm.nih.gov/pubmed/35496790
http://dx.doi.org/10.1016/j.xpro.2022.101342
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