Cargando…
Lysophosphatidylcholine induces azurophil granule translocation via Rho/Rho kinase/F-actin polymerization in human neutrophils
Translocation of azurophil granules is pivotal for bactericidal activity of neutrophils, the first-line defense cells against pathogens. Previously, we reported that lysophosphatidylcholine (LPC), an endogenous lipid, enhances bactericidal activity of human neutrophils via increasing translocation o...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Physiological Society and The Korean Society of Pharmacology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046897/ https://www.ncbi.nlm.nih.gov/pubmed/35477545 http://dx.doi.org/10.4196/kjpp.2022.26.3.175 |
_version_ | 1784695615942295552 |
---|---|
author | Ham, Hwa-Yong Kang, Shin-Hae Song, Dong-Keun |
author_facet | Ham, Hwa-Yong Kang, Shin-Hae Song, Dong-Keun |
author_sort | Ham, Hwa-Yong |
collection | PubMed |
description | Translocation of azurophil granules is pivotal for bactericidal activity of neutrophils, the first-line defense cells against pathogens. Previously, we reported that lysophosphatidylcholine (LPC), an endogenous lipid, enhances bactericidal activity of human neutrophils via increasing translocation of azurophil granules. However, the precise mechanism of LPC-induced azurophil granule translocation was not fully understood. Treatment of neutrophil with LPC significantly increased CD63 (an azurophil granule marker) surface expression. Interestingly, cytochalasin B, an inhibitor of action polymerization, blocked LPC-induced CD63 surface expression. LPC increased F-actin polymerization. LPC-induced CD63 surface expression was inhibited by both a Rho specific inhibitor, Tat-C3 exoenzyme, and a Rho kinase (ROCK) inhibitor, Y27632 which also inhibited LPC-induced F-actin polymerization. LPC induced Rho-GTP activation. NSC23766, a Rac inhibitor, however, did not affect LPC-induced CD63 surface expression. Theses results suggest a novel regulatory mechanism for azurophil granule translocation where LPC induces translocation of azurophil granules via Rho/ROCK/F-actin polymerization pathway. |
format | Online Article Text |
id | pubmed-9046897 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | The Korean Physiological Society and The Korean Society of Pharmacology |
record_format | MEDLINE/PubMed |
spelling | pubmed-90468972022-05-10 Lysophosphatidylcholine induces azurophil granule translocation via Rho/Rho kinase/F-actin polymerization in human neutrophils Ham, Hwa-Yong Kang, Shin-Hae Song, Dong-Keun Korean J Physiol Pharmacol Original Article Translocation of azurophil granules is pivotal for bactericidal activity of neutrophils, the first-line defense cells against pathogens. Previously, we reported that lysophosphatidylcholine (LPC), an endogenous lipid, enhances bactericidal activity of human neutrophils via increasing translocation of azurophil granules. However, the precise mechanism of LPC-induced azurophil granule translocation was not fully understood. Treatment of neutrophil with LPC significantly increased CD63 (an azurophil granule marker) surface expression. Interestingly, cytochalasin B, an inhibitor of action polymerization, blocked LPC-induced CD63 surface expression. LPC increased F-actin polymerization. LPC-induced CD63 surface expression was inhibited by both a Rho specific inhibitor, Tat-C3 exoenzyme, and a Rho kinase (ROCK) inhibitor, Y27632 which also inhibited LPC-induced F-actin polymerization. LPC induced Rho-GTP activation. NSC23766, a Rac inhibitor, however, did not affect LPC-induced CD63 surface expression. Theses results suggest a novel regulatory mechanism for azurophil granule translocation where LPC induces translocation of azurophil granules via Rho/ROCK/F-actin polymerization pathway. The Korean Physiological Society and The Korean Society of Pharmacology 2022-05-01 2022-05-01 /pmc/articles/PMC9046897/ /pubmed/35477545 http://dx.doi.org/10.4196/kjpp.2022.26.3.175 Text en Copyright © Korean J Physiol Pharmacol https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Ham, Hwa-Yong Kang, Shin-Hae Song, Dong-Keun Lysophosphatidylcholine induces azurophil granule translocation via Rho/Rho kinase/F-actin polymerization in human neutrophils |
title | Lysophosphatidylcholine induces azurophil granule translocation via Rho/Rho kinase/F-actin polymerization in human neutrophils |
title_full | Lysophosphatidylcholine induces azurophil granule translocation via Rho/Rho kinase/F-actin polymerization in human neutrophils |
title_fullStr | Lysophosphatidylcholine induces azurophil granule translocation via Rho/Rho kinase/F-actin polymerization in human neutrophils |
title_full_unstemmed | Lysophosphatidylcholine induces azurophil granule translocation via Rho/Rho kinase/F-actin polymerization in human neutrophils |
title_short | Lysophosphatidylcholine induces azurophil granule translocation via Rho/Rho kinase/F-actin polymerization in human neutrophils |
title_sort | lysophosphatidylcholine induces azurophil granule translocation via rho/rho kinase/f-actin polymerization in human neutrophils |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046897/ https://www.ncbi.nlm.nih.gov/pubmed/35477545 http://dx.doi.org/10.4196/kjpp.2022.26.3.175 |
work_keys_str_mv | AT hamhwayong lysophosphatidylcholineinducesazurophilgranuletranslocationviarhorhokinasefactinpolymerizationinhumanneutrophils AT kangshinhae lysophosphatidylcholineinducesazurophilgranuletranslocationviarhorhokinasefactinpolymerizationinhumanneutrophils AT songdongkeun lysophosphatidylcholineinducesazurophilgranuletranslocationviarhorhokinasefactinpolymerizationinhumanneutrophils |