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Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection
Nonhuman primates (NHP) are particularly important for modeling infections with viruses that do not naturally replicate in rodent cells. Zika virus (ZIKV) has been responsible for sporadic epidemics, but in 2015 a disseminated outbreak of ZIKV resulted in the World Health Organization declaring it a...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046911/ https://www.ncbi.nlm.nih.gov/pubmed/35493734 http://dx.doi.org/10.3389/fcimb.2022.880860 |
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author | Mask, Emma Hodara, Vida L. Callery, Jessica E. Parodi, Laura M. Obregon-Perko, Veronica Yagi, Shigeo Glenn, Jeremy Frost, Patrice Clemmons, Elizabeth Patterson, Jean L. Cox, Laura A. Giavedoni, Luis D. |
author_facet | Mask, Emma Hodara, Vida L. Callery, Jessica E. Parodi, Laura M. Obregon-Perko, Veronica Yagi, Shigeo Glenn, Jeremy Frost, Patrice Clemmons, Elizabeth Patterson, Jean L. Cox, Laura A. Giavedoni, Luis D. |
author_sort | Mask, Emma |
collection | PubMed |
description | Nonhuman primates (NHP) are particularly important for modeling infections with viruses that do not naturally replicate in rodent cells. Zika virus (ZIKV) has been responsible for sporadic epidemics, but in 2015 a disseminated outbreak of ZIKV resulted in the World Health Organization declaring it a global health emergency. Since the advent of this last epidemic, several NHP species, including the baboon, have been utilized for modeling and understanding the complications of ZIKV infection in humans; several health issues related to the outcome of infection have not been resolved yet and require further investigation. This study was designed to validate, in baboons, the molecular signatures that have previously been identified in ZIKV-infected humans and macaque models. We performed a comprehensive molecular analysis of baboons during acute ZIKV infection, including flow cytometry, cytokine, immunological, and transcriptomic analyses. We show here that, similar to most human cases, ZIKV infection of male baboons tends to be subclinical, but is associated with a rapid and transient antiviral interferon-based response signature that induces a detectable humoral and cell-mediated immune response. This immunity against the virus protects animals from challenge with a divergent ZIKV strain, as evidenced by undetectable viremia but clear anamnestic responses. These results provide additional support for the use of baboons as an alternative animal model to macaques and validate omic techniques that could help identify the molecular basis of complications associated with ZIKV infections in humans. |
format | Online Article Text |
id | pubmed-9046911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-90469112022-04-29 Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection Mask, Emma Hodara, Vida L. Callery, Jessica E. Parodi, Laura M. Obregon-Perko, Veronica Yagi, Shigeo Glenn, Jeremy Frost, Patrice Clemmons, Elizabeth Patterson, Jean L. Cox, Laura A. Giavedoni, Luis D. Front Cell Infect Microbiol Cellular and Infection Microbiology Nonhuman primates (NHP) are particularly important for modeling infections with viruses that do not naturally replicate in rodent cells. Zika virus (ZIKV) has been responsible for sporadic epidemics, but in 2015 a disseminated outbreak of ZIKV resulted in the World Health Organization declaring it a global health emergency. Since the advent of this last epidemic, several NHP species, including the baboon, have been utilized for modeling and understanding the complications of ZIKV infection in humans; several health issues related to the outcome of infection have not been resolved yet and require further investigation. This study was designed to validate, in baboons, the molecular signatures that have previously been identified in ZIKV-infected humans and macaque models. We performed a comprehensive molecular analysis of baboons during acute ZIKV infection, including flow cytometry, cytokine, immunological, and transcriptomic analyses. We show here that, similar to most human cases, ZIKV infection of male baboons tends to be subclinical, but is associated with a rapid and transient antiviral interferon-based response signature that induces a detectable humoral and cell-mediated immune response. This immunity against the virus protects animals from challenge with a divergent ZIKV strain, as evidenced by undetectable viremia but clear anamnestic responses. These results provide additional support for the use of baboons as an alternative animal model to macaques and validate omic techniques that could help identify the molecular basis of complications associated with ZIKV infections in humans. Frontiers Media S.A. 2022-04-14 /pmc/articles/PMC9046911/ /pubmed/35493734 http://dx.doi.org/10.3389/fcimb.2022.880860 Text en Copyright © 2022 Mask, Hodara, Callery, Parodi, Obregon-Perko, Yagi, Glenn, Frost, Clemmons, Patterson, Cox and Giavedoni https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Mask, Emma Hodara, Vida L. Callery, Jessica E. Parodi, Laura M. Obregon-Perko, Veronica Yagi, Shigeo Glenn, Jeremy Frost, Patrice Clemmons, Elizabeth Patterson, Jean L. Cox, Laura A. Giavedoni, Luis D. Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection |
title | Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection |
title_full | Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection |
title_fullStr | Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection |
title_full_unstemmed | Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection |
title_short | Molecular Approaches for the Validation of the Baboon as a Nonhuman Primate Model for the Study of Zika Virus Infection |
title_sort | molecular approaches for the validation of the baboon as a nonhuman primate model for the study of zika virus infection |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046911/ https://www.ncbi.nlm.nih.gov/pubmed/35493734 http://dx.doi.org/10.3389/fcimb.2022.880860 |
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