Cargando…

Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method(SM) 082101

BACKGROUND: The 3M(™) Petrifilm(™) Coliform Count (CC) Plate is a sample-ready-culture medium system which contains modified Violet Red Bile nutrients, a cold-water-soluble gelling agent, and a tetrazolium indicator that facilitates colony enumeration. OBJECTIVE: To validate the 3M Petrifilm CC Plat...

Descripción completa

Detalles Bibliográficos
Autores principales: Schumacher, April J, Lingle, Cari K, Silbernagel, Karen M, Thompson-Strehlow, Leslie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046964/
https://www.ncbi.nlm.nih.gov/pubmed/34677581
http://dx.doi.org/10.1093/jaoacint/qsab137
_version_ 1784695631320711168
author Schumacher, April J
Lingle, Cari K
Silbernagel, Karen M
Thompson-Strehlow, Leslie
author_facet Schumacher, April J
Lingle, Cari K
Silbernagel, Karen M
Thompson-Strehlow, Leslie
author_sort Schumacher, April J
collection PubMed
description BACKGROUND: The 3M(™) Petrifilm(™) Coliform Count (CC) Plate is a sample-ready-culture medium system which contains modified Violet Red Bile nutrients, a cold-water-soluble gelling agent, and a tetrazolium indicator that facilitates colony enumeration. OBJECTIVE: To validate the 3M Petrifilm CC Plate method for bottled water through the AOAC(®)  Performance Tested Method(s)(SM) program. METHODS: The performance of the 3M Petrifilm CC Plate was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 4, Section III.D for the enumeration of coliforms in bottled water. Matrix data were normalized by log(10) transformation and performance indicators included repeatability and difference of means with 90 and 95% confidence intervals. Inclusivity, exclusivity, robustness, and product consistency and stability were evaluated. RESULTS: This study demonstrated that the 3M Petrifilm CC Plate method detects and enumerates coliforms from bottled water. The average log(10) counts of the 3M Petrifilm CC Plate method were equivalent to or better than the average log(10) counts of the reference method. Results from inclusivity and exclusivity studies demonstrated that the 3M Petrifilm CC Plate method differentiated coliforms from non-coliform strains. In product consistency, stability, and robustness testing, different lots of 3M Petrifilm CC Plates and small deviations in incubation time and temperature did not affect test results. CONCLUSION: The 3M Petrifilm CC Plate method is an accurate and robust method for the enumeration of coliforms in bottled water. HIGHLIGHTS: The 3M Petrifilm CC Plate allows for detection of confirmed coliforms within 24 h. Up to 20 sample-ready plates can be stacked during incubation, providing flexibility and saving space.
format Online
Article
Text
id pubmed-9046964
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-90469642022-04-28 Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method(SM) 082101 Schumacher, April J Lingle, Cari K Silbernagel, Karen M Thompson-Strehlow, Leslie J AOAC Int Microbiological Methods BACKGROUND: The 3M(™) Petrifilm(™) Coliform Count (CC) Plate is a sample-ready-culture medium system which contains modified Violet Red Bile nutrients, a cold-water-soluble gelling agent, and a tetrazolium indicator that facilitates colony enumeration. OBJECTIVE: To validate the 3M Petrifilm CC Plate method for bottled water through the AOAC(®)  Performance Tested Method(s)(SM) program. METHODS: The performance of the 3M Petrifilm CC Plate was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 4, Section III.D for the enumeration of coliforms in bottled water. Matrix data were normalized by log(10) transformation and performance indicators included repeatability and difference of means with 90 and 95% confidence intervals. Inclusivity, exclusivity, robustness, and product consistency and stability were evaluated. RESULTS: This study demonstrated that the 3M Petrifilm CC Plate method detects and enumerates coliforms from bottled water. The average log(10) counts of the 3M Petrifilm CC Plate method were equivalent to or better than the average log(10) counts of the reference method. Results from inclusivity and exclusivity studies demonstrated that the 3M Petrifilm CC Plate method differentiated coliforms from non-coliform strains. In product consistency, stability, and robustness testing, different lots of 3M Petrifilm CC Plates and small deviations in incubation time and temperature did not affect test results. CONCLUSION: The 3M Petrifilm CC Plate method is an accurate and robust method for the enumeration of coliforms in bottled water. HIGHLIGHTS: The 3M Petrifilm CC Plate allows for detection of confirmed coliforms within 24 h. Up to 20 sample-ready plates can be stacked during incubation, providing flexibility and saving space. Oxford University Press 2021-10-22 /pmc/articles/PMC9046964/ /pubmed/34677581 http://dx.doi.org/10.1093/jaoacint/qsab137 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Microbiological Methods
Schumacher, April J
Lingle, Cari K
Silbernagel, Karen M
Thompson-Strehlow, Leslie
Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method(SM) 082101
title Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method(SM) 082101
title_full Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method(SM) 082101
title_fullStr Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method(SM) 082101
title_full_unstemmed Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method(SM) 082101
title_short Validation of the 3M™ Petrifilm™ Coliform Count Plate for Enumeration of Coliforms in Bottled Water: AOAC Performance Tested Method(SM) 082101
title_sort validation of the 3m™ petrifilm™ coliform count plate for enumeration of coliforms in bottled water: aoac performance tested method(sm) 082101
topic Microbiological Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9046964/
https://www.ncbi.nlm.nih.gov/pubmed/34677581
http://dx.doi.org/10.1093/jaoacint/qsab137
work_keys_str_mv AT schumacheraprilj validationofthe3mpetrifilmcoliformcountplateforenumerationofcoliformsinbottledwateraoacperformancetestedmethodsm082101
AT linglecarik validationofthe3mpetrifilmcoliformcountplateforenumerationofcoliformsinbottledwateraoacperformancetestedmethodsm082101
AT silbernagelkarenm validationofthe3mpetrifilmcoliformcountplateforenumerationofcoliformsinbottledwateraoacperformancetestedmethodsm082101
AT thompsonstrehlowleslie validationofthe3mpetrifilmcoliformcountplateforenumerationofcoliformsinbottledwateraoacperformancetestedmethodsm082101